{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:43:13Z","timestamp":1774615393345,"version":"3.50.1"},"reference-count":23,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"6","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Global Oncology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>PURPOSE<\/jats:title><jats:p> Cholangiocarcinoma (CCA) remains a disease with poor prognosis and limited therapeutic options. Identification of driver genetic alterations may lead to the discovery of more effective targeted therapies. CCAs harboring FGFR2 fusions have recently demonstrated promising responses to FGFR inhibitors, highlighting their potential relevance as predictive biomarkers. CCA incidence is high in the northeast of Thailand and its neighboring countries because of chronic infection with the liver fluke Opisthorchis viverrini (Ov). However, there are currently no available data on the prevalence of FGFR alterations in fluke-associated CCA in endemic countries. <\/jats:p><\/jats:sec><jats:sec><jats:title>MATERIALS AND METHODS<\/jats:title><jats:p> In this study, we performed anchored multiplex polymerase chain reaction target enrichment RNA sequencing of FGFR1-3, validated by fluorescence in situ hybridization and Sanger sequencing, in 121 Ov-associated and 95 non\u2013Ov-associated CCA tumors. <\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p> Compared with non\u2013fluke-associated CCA (11\/95; 11.6%), FGFR2 fusions were significantly less common in fluke-associated CCA (1\/121; 0.8%; P = .0006). All FGFR fusions were detected exclusively in intrahepatic CCAs and were mutually exclusive with KRAS\/ERBB2\/BRAF\/FGFR mutations, pointing to their potential roles as oncogenic drivers. <\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p> FGFR2 fusions are rare in fluke-associated CCA, underscoring how distinct etiologies may affect molecular landscapes in tumors and highlighting the need to discover other actionable genomic alterations in endemic fluke-associated CCA. <\/jats:p><\/jats:sec>","DOI":"10.1200\/go.20.00030","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T20:06:16Z","timestamp":1587499576000},"page":"628-638","update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":45,"title":["Lack of Targetable FGFR2 Fusions in Endemic Fluke-Associated Cholangiocarcinoma"],"prefix":"10.1200","author":[{"given":"Sarinya","family":"Kongpetch","sequence":"first","affiliation":[{"name":"Cholangiocarcinoma Screening and Care Program and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Department of Pharmacology, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apinya","family":"Jusakul","sequence":"additional","affiliation":[{"name":"Cholangiocarcinoma Screening and Care Program and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand"},{"name":"The Centre for Research and Development of Medical Diagnostic Laboratories and Department of Clinical Immunology and Transfusion Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing Quan","family":"Lim","sequence":"additional","affiliation":[{"name":"Oncology Academic Clinical Program, Duke-NUS Medical School, Singapore"},{"name":"Lymphoma Genomic Translational Research Laboratory, Division of Cellular and Molecular Research, National Cancer Centre Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cedric Chuan Young","family":"Ng","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Epigenome, Division of Medical Science, National Cancer Centre Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason Yongsheng","family":"Chan","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology, National Cancer Centre Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikneswari","family":"Rajasegaran","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Epigenome, Division of Medical Science, National Cancer Centre Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tse Hui","family":"Lim","sequence":"additional","affiliation":[{"name":"Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiat Hon","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology, Singapore General Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su Pin","family":"Choo","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology, National Cancer Centre Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Dima","sequence":"additional","affiliation":[{"name":"Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irinel","family":"Popescu","sequence":"additional","affiliation":[{"name":"Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan G.","family":"Duda","sequence":"additional","affiliation":[{"name":"Edwin L. 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