{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:20:33Z","timestamp":1770823233544,"version":"3.50.1"},"reference-count":50,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2000,2,1]]},"abstract":"<jats:p> PURPOSE: t(8;14)(q24;q32) and\/or c-MYC\/immunoglobulin heavy-chain (IGH) fusion gene have been observed not only in Burkitt\u2019s lymphoma (BL) but also in a proportion of non-BL, diffuse large-cell lymphoma of B-cell type (DLCL). We explored molecular features of DLCL with c-MYC\/IGH fusion and the impact of this genetic abnormality on clinical outcome of DLCL. <\/jats:p><jats:p> PATIENTS AND METHODS: A total of 203 cases of non-BL DLCL were studied. Genomic DNA extracted from tumor tissues was subjected to long-distance polymerase chain reaction (LD-PCR) using oligonucleotide primers for exon 2 of c-MYC and for the four constant region genes of IGH. <\/jats:p><jats:p> RESULTS: Twelve cases (5.9%) showed positive amplification; one had a c-MYC\/C\u03bc, nine had a c-MYC\/C\u03b3, and two had a c-MYC\/C\u03b1 fusion sequence. Restriction and sequence analysis of the LD-PCR products, ranging from 2.3 to 9.4 kb in size, showed that breakage in the 12 cases occurred within a 1.5-kb region that included exon 1 of c-MYC in combination with breakpoints at the switch regions of IGH (10 of 12). In 10 cases, Myc protein encoded by the fusion genes demonstrated mutations and\/or deletions. Six cases had additional molecular lesions in BCL-2 or BCL-6 and\/or p53 genes. The age range of the 12 patients was 44 to 86 years, with a median age of 65.5 years. Five patients had stage I\/II disease, and seven had stage III\/IV disease. Lactate dehydrogenase was elevated in nine of 11 subjects. Seven showed involvement of the gastrointestinal tract. All patients were treated by surgery and\/or chemoradiotherapy; six died of the disease within 1 year, resulting in the poorest 1- and 2-year survival rates among DLCL subgroups. <\/jats:p><jats:p> CONCLUSION: The c-MYC\/IGH fusion gene of DLCL is identical to that of the sporadic type of BL (sBL). DLCL with c-MYC\/IGH shares clinical features with sBL but is characterized further by an older age distribution. <\/jats:p>","DOI":"10.1200\/jco.2000.18.3.510","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T07:04:24Z","timestamp":1487919864000},"page":"510-510","source":"Crossref","is-referenced-by-count":109,"title":["Molecular and Clinical Features of Non-Burkitt\u2019s, Diffuse Large-Cell Lymphoma of B-Cell Type Associated With the c-MYC\/Immunoglobulin Heavy-Chain Fusion Gene"],"prefix":"10.1200","volume":"18","author":[{"given":"Takashi","family":"Akasaka","sequence":"first","affiliation":[{"name":"From the First DivisionDepartment of Internal Medicine, and Laboratory of Anatomical Pathology, Faculty of Medicine, The Center for Molecular Biology and Genetics, Kyoto University, Kyoto; and First Department of Internal Medicine, Kansai Medical University, Moriguchi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Akasaka","sequence":"additional","affiliation":[{"name":"From the First DivisionDepartment of Internal Medicine, and Laboratory of Anatomical Pathology, Faculty of Medicine, The Center for Molecular Biology and Genetics, Kyoto University, Kyoto; and First Department of Internal Medicine, Kansai Medical University, Moriguchi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiyoko","family":"Ueda","sequence":"additional","affiliation":[{"name":"From the First DivisionDepartment of Internal Medicine, and Laboratory of Anatomical Pathology, Faculty of Medicine, The Center for Molecular Biology and Genetics, Kyoto University, Kyoto; and First Department of Internal Medicine, Kansai Medical University, Moriguchi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Yonetani","sequence":"additional","affiliation":[{"name":"From the First DivisionDepartment of Internal Medicine, and Laboratory of Anatomical Pathology, Faculty of Medicine, The Center for Molecular Biology and Genetics, Kyoto University, Kyoto; and First Department of Internal Medicine, Kansai Medical University, Moriguchi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitomo","family":"Maesako","sequence":"additional","affiliation":[{"name":"From the First DivisionDepartment of Internal Medicine, and Laboratory of Anatomical Pathology, Faculty of Medicine, The Center for Molecular Biology and Genetics, Kyoto University, Kyoto; 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