{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:48:33Z","timestamp":1761101313098},"reference-count":42,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:p> PURPOSE: The role of donor marrow T-cell depletion (TCD) in preventing graft-versus-host disease (GVHD) after transplantation of unrelated allogeneic marrow remains undefined. Because different TCD methodologies differ in the degree and specificity with which T cells are removed, it is likely that transplant outcomes would depend on which technique is used. Herein, we report results in the first 48 recipients of unrelated marrow using CD6+ TCD as the sole form of GVHD prophylaxis. <\/jats:p><jats:p> PATIENTS AND METHODS: Median age of patients was 46 years (20 to 58 years). Donors were matched at A\/B HLA loci. Ablation consisted of cyclophosphamide and fractionated total-body irradiation (TBI; 14 Gy). To facilitate engraftment, patients also received 7.5 Gy (22 points) or 4.5 Gy (26 points) of total lymphoid irradiation (TLI) before admission. No additional immune suppressive prophylaxis was administered. Granulocyte colony-stimulating factor was administered daily from day +1 to engraftment. <\/jats:p><jats:p> RESULTS: All 48 patients demonstrated neutrophil engraftment. An absolute neutrophil count of 500 \u00d7 10<jats:sup>6<\/jats:sup>\/L was achieved at a median of 12 days (range, 9 to 23 days). There were no cases of late graft failure. The number of CD34+ cells infused\/kg was associated with speed of platelet and neutrophil recovery. The dose of TLI did not influence engraftment. Grades 2-4 acute GVHD occurred in 42% of patients (95% confidence interval [CI], 0.28 to 0.57). Mortality at day 100 was 19%. There have been only five relapses. Estimated 2-year survival was 44% (95% CI, 0.28 to 0.59) for the entire group, 58% for patients less than 50 years of age. In multivariable analysis, age less than 50 years (P = .002), cytomegalovirus seronegative status (P = .04), and early disease status at bone marrow transplant (P = .05) were associated with superior survival. <\/jats:p><jats:p> CONCLUSION: CD6+ TCD does not impede engraftment of unrelated bone marrow after low-dose TLI, cyclophosphamide, and TBI. CD6+ TCD as the sole form of GVHD prophylaxis results in an incidence of GVHD that compares favorably with many adult studies of unrelated transplantation using unmanipulated marrow and immune-suppressive medications, especially in light of the median age of our patients (46 years). Although event-free survival in patients less than 50 years of age is very encouraging, older patients experience frequent transplantation-related complications despite TCD. <\/jats:p>","DOI":"10.1200\/jco.2001.19.4.1152","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T06:36:16Z","timestamp":1487918176000},"page":"1152-1159","source":"Crossref","is-referenced-by-count":43,"title":["CD6+ Donor Marrow T-Cell Depletion as the Sole Form of Graft-Versus-Host Disease Prophylaxis in Patients Undergoing Allogeneic Bone Marrow Transplant From Unrelated Donors"],"prefix":"10.1200","volume":"19","author":[{"given":"Robert J.","family":"Soiffer","sequence":"first","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edie","family":"Weller","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin P.","family":"Alyea","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Mauch","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iain L.","family":"Webb","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.","family":"Fisher","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnold S.","family":"Freedman","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Schlossman","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Gribben","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Lee","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s Hospital, Boston, MA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"From the Departments of Adult Oncology, Radiation Oncology, and Biostatistics, Dana-Farber Cancer Institute\/Brigham and Women\u2019s 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