{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:35:47Z","timestamp":1783625747081,"version":"3.55.0"},"reference-count":47,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p> PURPOSE: To prospectively compare cytogenetics and reverse transcriptase\u2013polymerase chain reaction (RT-PCR) for detection of t(8;21)(q22;q22) and inv(16)(p13q22)\/t(16;16)(p13;q22), aberrations characteristic of core-binding factor (CBF) acute myeloid leukemia (AML), in 284 adults newly diagnosed with primary AML. <\/jats:p><jats:p> PATIENTS AND METHODS: Cytogenetic analyses were performed at local laboratories, with results reviewed centrally. RT-PCR for AML1\/ETO and CBF\u03b2\/MYH11 was performed centrally. <\/jats:p><jats:p> RESULTS: CBF AML was ultimately identified in 48 patients: 21 had t(8;21) or its variant and AML1\/ETO, and 27 had inv(16)\/t(16;16), CBF\u03b2\/MYH11, or both. Initial cytogenetic and RT-PCR analyses correctly classified 95.7% and 96.1% of patients, respectively (P = .83). Initial cytogenetic results were considered to be false-negative in three AML1\/ETO-positive patients with unique variants of t(8;21), and in three CBF\u03b2\/MYH11-positive patients with, respectively, an isolated +22; del(16)(q22),+22; and a normal karyotype. The latter three patients were later confirmed to have inv(16)\/t(16;16) cytogenetically. Only one of 124 patients reported initially as cytogenetically normal was ultimately RT-PCR\u2013positive. There was no false-positive cytogenetic result. Initial RT-PCR was falsely negative in two patients with inv(16) and falsely positive for AML1\/ETO in two and for CBF\u03b2\/MYH11 in another two patients. Two patients with del(16)(q22) were found to be CBF\u03b2\/MYH11-negative. M4Eo marrow morphology was a good predictor of the presence of inv(16)\/t(16;16). <\/jats:p><jats:p> CONCLUSION: Patients with t(8;21) or inv(16) can be successfully identified in prospective multi-institutional clinical trials. Both cytogenetics and RT-PCR detect most such patients, although each method has limitations. RT-PCR is required when the cytogenetic study fails; it is also required to determine whether patients with suspected variants of t(8;21), del(16)(q22), or +22 represent CBF AML. RT-PCR should not replace cytogenetics and should not be used as the only diagnostic test for detection of CBF AML because of the possibility of obtaining false-positive or false-negative results. <\/jats:p>","DOI":"10.1200\/jco.2001.19.9.2482","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T06:55:01Z","timestamp":1487919301000},"page":"2482-2492","source":"Crossref","is-referenced-by-count":81,"title":["Comparison of Cytogenetic and Molecular Genetic Detection of t(8;21) and inv(16) in a Prospective Series of Adults With De Novo Acute Myeloid Leukemia: A Cancer and Leukemia Group B Study"],"prefix":"10.1200","volume":"19","author":[{"given":"Krzysztof","family":"Mr\u00f3zek","sequence":"first","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas W.","family":"Prior","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Edwards","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Marcucci","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Carroll","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"Snyder","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad R.K.","family":"Koduru","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl S.","family":"Theil","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Pettenati","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellie J.","family":"Archer","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Caligiuri","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Vardiman","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Kolitz","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Larson","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; Wake Forest University Medical Center, Winston-Salem, NC; and Department of Pathology, University of Chicago, Chicago, IL."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara D.","family":"Bloomfield","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Oncology, Comprehensive Cancer Center, and Department of Pathology, Ohio State University, Columbus, OH; University of Alabama at Birmingham, Birmingham, AL; North Shore University Hospital, Manhasset, NY; 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