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Multidrug resistance (MDR)-associated genes are known to be inducible by heat and drugs; expression levels of the major vault protein (MVP), MDR1, and MDR-associated protein 1 (MRP1) were determined sequentially before, during, and after ILP of patients.<\/jats:p><jats:p>PATIENTS AND METHODS: Twenty-one STS or malignant melanoma patients were treated by ILP. Tumor tissue temperatures were recorded continuously and ranged from 33.4\u00b0C initially to peak values of 40.4\u00b0C during ILP. Serial true-cut biopsy specimens from tumor tissues were routinely microdissected. Expression analyses for MDR genes were performed by real-time reverse transcriptase polymerase chain reaction and immunohistochemistry.<\/jats:p><jats:p>RESULTS: In 83% of the patients, MVP expression was induced during hyperthermic ILP. MVP-mRNA inductions often paralleled the increase in temperature during ILP. Increased MVP protein expressions either were observed simultaneously with the MVP-mRNA induction or were delayed until after the induction at the transcriptional level. Inductions of MDR1 and MRP1 were observed in only 13% and 27% of the specimens analyzed. Temperatures and drugs applied preferentially led to an induction of MVP and were not sufficient to induce MDR1 and MRP1 in the majority of tumors.<\/jats:p><jats:p>CONCLUSION: This study is the first to analyze the expression of MDR-associated genes sequentially during ILP of patients and demonstrates that treatment might lead to increased levels of MVP, whereas enhanced levels of MDR1 and MRP1 remain rare events.<\/jats:p>","DOI":"10.1200\/jco.2002.01.003","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T21:58:26Z","timestamp":1033509506000},"page":"3282-3292","source":"Crossref","is-referenced-by-count":38,"title":["Expression of Multidrug Resistance Genes<i>MVP<\/i>,<i>MDR1<\/i>, and<i>MRP1<\/i>Determined Sequentially Before, During, and After Hyperthermic Isolated Limb Perfusion of Soft Tissue Sarcoma and Melanoma Patients"],"prefix":"10.1200","volume":"20","author":[{"given":"Ulrike","family":"Stein","sequence":"first","affiliation":[{"name":"From the Division of Surgery and Surgical Oncology, Charit\u00e9, Humboldt University, Campus Berlin-Buch, Robert R\u00f6ssle Hospital and Tumor Institute, and Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"J\u00fcrchott","sequence":"additional","affiliation":[{"name":"From the Division of Surgery and Surgical Oncology, Charit\u00e9, Humboldt University, Campus Berlin-Buch, Robert R\u00f6ssle Hospital and Tumor Institute, and Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Schl\u00e4fke","sequence":"additional","affiliation":[{"name":"From the Division of Surgery and Surgical Oncology, Charit\u00e9, Humboldt University, Campus Berlin-Buch, Robert R\u00f6ssle Hospital and Tumor Institute, and Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Hohenberger","sequence":"additional","affiliation":[{"name":"From the Division of Surgery and Surgical Oncology, Charit\u00e9, Humboldt University, Campus Berlin-Buch, Robert R\u00f6ssle Hospital and Tumor Institute, and Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"233","reference":[{"key":"R1-111468","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1992.10.1.52"},{"key":"R2-111468","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.10.2653"},{"key":"R3-111468","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199612000-00011"},{"key":"R4-111468","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1047593"},{"key":"R5-111468","unstructured":"Lienard D, Eggermont AMM, Schraffordt Koops H, et al: Isolated perfusion of the limb with high-dose tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and melphalan for melanoma stage III: Results of a multi-centre pilot study. 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