{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:16:47Z","timestamp":1791998207657,"version":"4.4.0"},"reference-count":27,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> Gastric marginal-zone B-cell lymphoma (MZBCL) of the mucosa-associated lymphoid tissue (MALT) is associated with chronic Helicobacter pylori gastritis. Stable complete remission (CR) can be induced by H pylori eradication. Whether this is paralleled by cure of the lymphoma remains unclear. Persisting monoclonal bands for immunoglobulin heavy chain variable region (V<jats:sub>H<\/jats:sub>) representing the lymphoma clone have been described in up to 50% of patients in CR. This retrospective study investigated whether this phenomenon also occurs after radiochemotherapy. <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> Biopsy samples of 20 patients receiving chemotherapy with cyclophosphamide, doxorubicin, vincristine, and prednisone and irradiation were analyzed before and after therapy. Study patients had Ann Arbor stage I\/II primary gastric cancer, including four cases of MZBCL of MALT type, 12 cases of diffuse large-cell lymphomas (DLCL), and four cases of mixed MALT type\/DLCL. Polymerase chain reaction (PCR) for V<jats:sub>H<\/jats:sub> rearrangement was performed. Monoclonal PCR products were cloned and sequenced. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> Fourteen of 20 patients had a monoclonal or oligoclonal band distribution at diagnosis converted into polyclonal pattern after radiochemotherapy. Of the remaining six patients, two were lost to follow-up. One patient did not respond and died of progressive disease. PCR in this patient showed persistent B-cell clonality. In three patients, the initial PCR showed a polyclonal pattern and thus could not be evaluated during follow-up. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> In contrast with H pylori eradication alone, radiochemotherapy results in clearing of monoclonal cells during follow-up. This may result in better elimination of residual lymphoma cells. Further study is needed to determine whether this translates into lower risk of relapse. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2004.08.177","type":"journal-article","created":{"date-parts":[[2004,7,30]],"date-time":"2004-07-30T06:39:56Z","timestamp":1091169596000},"page":"3039-3045","source":"Crossref","is-referenced-by-count":8,"title":["Ongoing Monoclonal B-Cell Proliferation Is Not Common in Gastric B-Cell Lymphoma After Combined Radiochemotherapy"],"prefix":"10.1200","volume":"22","author":[{"given":"Birgit","family":"Alpen","sequence":"first","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Kuse","sequence":"additional","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radzak","family":"Parwaresch","sequence":"additional","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Konrad","family":"M\u00fcller-Hermelink","sequence":"additional","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Stolte","sequence":"additional","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Neubauer","sequence":"additional","affiliation":[{"name":"From the Departments of Internal Medicine, Hematology, Oncology, and Immunology, Hospital of the Philipps-University, Marburg; Department of Hematology, St Georg Hospital, Hamburg; Institute for Hematopathology, University of Kiel, Kiel; Institute for Pathology, University of W\u00fcrzburg, W\u00fcrzburg; and Institute for Pathology, Hospital Bayreuth, Bayreuth, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"233","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19831015)52:8<1410::AID-CNCR2820520813>3.0.CO;2-3"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92035-Z"},{"key":"R3","unstructured":"Hussell T, Isaacson PG, Crabtree JE, et al: Immunoglobulin specificity of low-grade B cell gastrointestinal lymphoma of mucosa-associated lymphoid tissue (MALT) type. 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