{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:49:52Z","timestamp":1792633792978,"version":"4.5.0"},"reference-count":56,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2004,9,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> To determine the clinicopathologic significance and prognostic value of chromosomal imbalances in diffuse large B-cell lymphomas (DLBCL). <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> We have examined 64 tumors at diagnosis using comparative genomic hybridization and real-time quantitative polymerase chain reaction (PCR), single-stranded conformational polymorphism, and DNA sequencing for the analysis of several potential target genes. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> The most recurrent alterations were gains of 18q (20%), Xq (15%), 2p, 7q, and 12p (14%), and losses of 6q and 17p (14%). Frequent high-level DNA amplifications were detected at 2p13-p16 and 18q21 loci. Real-time quantitative PCR detected REL and BCL11A gene amplifications in the nine patients with gains at 2p13-p16 and only in one additional patient with normal chromosome 2. Similarly, the BCL-2 gene was amplified in the 12 tumors with gains of 18q21 but in none of 39 patients with normal 18q profile. p53 gene inactivation was detected in nine of 58 (16%) tumors and was commonly associated with 17p losses. Tumors with 18q gains were significantly associated with a high number of chromosomal imbalances, primary nodal presentation, high serum lactate dehydrogenase levels, high International Prognostic Index, shorter cause-specific survival, and a high risk of relapse. Losses of 17p and p53 gene alterations were associated with an absence of complete response achievement. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> These results suggest that DLBCLs have a characteristic pattern of genomic alterations; 18q gains or amplifications and 17p losses are associated with particular clinicopathological features and aggressive clinical behavior. Additional studies are needed to confirm these observations in larger series of patients. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2004.11.025","type":"journal-article","created":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T22:33:03Z","timestamp":1093905183000},"page":"3498-3506","source":"Crossref","is-referenced-by-count":75,"title":["Clinicopathologic Significance and Prognostic Value of Chromosomal Imbalances in Diffuse Large B-Cell Lymphomas"],"prefix":"10.1200","volume":"22","author":[{"given":"S\u00edlvia","family":"Be\u00e0","sequence":"first","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Llu\u00eds","family":"Colomo","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"L\u00f3pez-Guillermo","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itziar","family":"Salaverria","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Puig","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Pinyol","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Rives","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emili","family":"Montserrat","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias","family":"Campo","sequence":"additional","affiliation":[{"name":"From the Hematopathology Section, Laboratory of Pathology and Hematology Department, Hospital Cl\u00ednic, Institut d'Investigacions Biomediques August Pi I Sunyer, University of Barcelona, Barcelona; and Information and Studies Service, Department of Health and Social Security, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"233","reference":[{"key":"R1","unstructured":"Jaffe ES, Harris NL, Stein H, et al: (eds). World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues . Lyon, France, IARC Press, 2001"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199304083281409"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199309303291402"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199906)25:2<123::AID-GCC8>3.0.CO;2-4"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.7.2285"},{"key":"R6","unstructured":"Schlegelberger B, Zwingers T, Harder L, et al: Clinicopathogenetic significance of chromosomal abnormalities in patients with blastic peripheral B-cell lymphoma: Kiel-Wien-Lymphoma Study Group. Blood 94:3114,1999-3120,"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(18)30389-7"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870030304"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/0268-960X(88)90027-6"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198904203201605"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.1.203.bloodjournal851203"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000024375.04135.2B"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1638"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.12.5269.bloodjournal87125269"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.1.234.413k22_234_240"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(01)00548-9"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(02)00843-9"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.7.2554"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2001.2164"},{"key":"R20","unstructured":"Bea S, Ribas M, Hernandez JM, et al: Increased number of chromosomal imbalances and high-level DNA amplifications in mantle cell lymphoma are associated with blastoid variants. Blood 93:4365,1999-4374,"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64256-3"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.12.006"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64140-5"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.12.3835"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1286"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65071-7"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Kaplan GL, Meier P: Non-parametric estimation from incomplete observations. J Am Stat Assoc 53:547,1958-581,","DOI":"10.1080\/01621459.1958.10501452"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.2307\/2529177"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Cox DR: Regression models and life tables. J R Stat Soc B 34:187,1972,","DOI":"10.1111\/j.2517-6161.1972.tb00899.x"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10016"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04502.x"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.1103"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10104"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206297"},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Weber T, Weber RG, Kaulich K, et al: Characteristic chromosomal imbalances in primary central nervous system lymphomas of the diffuse large B-cell type. Brain Pathol 10:73,2000-84,","DOI":"10.1111\/j.1750-3639.2000.tb00244.x"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.3.1168"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.3109\/10428190109097729"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401969"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401978"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1994.12.1.149"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.3.1046.1046"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.1.244"},{"key":"R43","doi-asserted-by":"crossref","unstructured":"Martinez-Climent JA, Alizadeh AA, Segraves R, et al: Transformation of follicular lymphoma to diffuse large cell lymphoma is associated with a heterogeneous set of DNA copy number and gene expression alterations. Blood 101:3109,2002-3117,","DOI":"10.1182\/blood-2002-07-2119"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199708213370804"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199911)26:3<203::AID-GCC3>3.0.CO;2-E"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.4.1571.bloodjournal8741571"},{"key":"R47","unstructured":"Chan WY, Wong N, Chan AB, et al: Consistent copy number gain in chromosome 12 in primary diffuse large cell lymphomas of the stomach. Am J Pathol 152:11,1998-16,"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.11.4321"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012914"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.1.25.25"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1381"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2577"},{"key":"R53","unstructured":"Mathew S, Murty VV, Dalla-Favera R, et al: Chromosomal localization of genes encoding the transcription factors, c-rel, NF-kappa Bp50, NF-kappa Bp65, and lyt-10 by fluorescence in situ hybridization. Oncogene 8:191,1993-193,"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.12.3413"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1038\/ni925"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1474"}],"container-title":["Journal of Clinical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ascopubs.org\/doi\/pdfdirect\/10.1200\/JCO.2004.11.025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T20:53:50Z","timestamp":1653512030000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascopubs.org\/doi\/10.1200\/JCO.2004.11.025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,1]]},"references-count":56,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2004,9,1]]}},"alternative-id":["10.1200\/JCO.2004.11.025"],"URL":"https:\/\/doi.org\/10.1200\/jco.2004.11.025","relation":{},"ISSN":["0732-183X","1527-7755"],"issn-type":[{"value":"0732-183X","type":"print"},{"value":"1527-7755","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,1]]}}}