{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T08:04:20Z","timestamp":1791014660494,"version":"4.1.0"},"reference-count":23,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2006,2,20]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> Serum B-lymphocyte stimulator (BLyS) levels have been found to be elevated in a number of immune disease models. Therefore, we sought to establish whether BLyS levels were elevated in patients with B-cell lymphoproliferative disorders and to determine whether elevated BLyS levels correlated with clinical characteristics of the disease. <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> Specimens were collected from the peripheral blood of individuals diagnosed with B-cell chronic lymphocytic leukemia (B-CLL; n = 70) or from age- and sex-matched patients seen at the same institution (n = 41). Serum BLyS levels were determined by enzyme-linked immunosorbent assay, and sequencing of the BLyS promoter was performed by conventional methods and confirmed by restriction fragment length polymorphism analysis. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> We found that elevated BLyS levels were more common in patients with familial B-CLL than individuals with sporadic B-CLL or normal controls. Because of this association, we sequenced the BLyS promoter in patients with B-CLL and normal controls and identified a polymorphic site, \u2212871 C\/T. We found that the wild-type sequence was significantly underrepresented in patients with familial B-CLL (4%) compared with patients with sporadic B-CLL (30%; P = .01) or controls (24%; P = .04). Furthermore, using a luciferase reporter under control of the BLyS promoter containing either a C or a T at position \u2212871, we found that the reporter construct containing a T at \u2212871 had a 2.6-fold increase in activity (P = .004). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> Our data suggest serum BLyS levels are elevated in patients with familial B-CLL and that elevated BLyS levels correlate with the presence of a T at \u2212871 in the BLyS promoter. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2005.02.7938","type":"journal-article","created":{"date-parts":[[2006,1,24]],"date-time":"2006-01-24T01:53:26Z","timestamp":1138067606000},"page":"983-987","source":"Crossref","is-referenced-by-count":75,"title":["Elevated Serum B-Lymphocyte Stimulator Levels in Patients With Familial Lymphoproliferative Disorders"],"prefix":"10.1200","volume":"24","author":[{"given":"Anne J.","family":"Novak","sequence":"first","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna M.","family":"Grote","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven C.","family":"Ziesmer","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Kline","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle K.","family":"Manske","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Slager","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Witzig","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tait","family":"Shanafelt","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy G.","family":"Call","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil E.","family":"Kay","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane F.","family":"Jelinek","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Cerhan","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane A.","family":"Gross","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Harder","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey R.","family":"Dillon","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; and Zymogenetics, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M.","family":"Ansell","sequence":"additional","affiliation":[{"name":"From the Division of Hematology and Internal Medicine, Department of Immunology, and Divisions of Epidemiology and Biostatistics, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN; 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