{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:54:04Z","timestamp":1777593244899,"version":"3.51.4"},"reference-count":19,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"30","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2008,10,20]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>A phase I\/II trial was performed to evaluate the safety and immunogenicity of a novel melanoma vaccine comprising six melanoma-associated peptides defined as antigenic targets for melanoma-reactive helper T cells. Source proteins for these peptides include MAGE proteins, MART-1\/MelanA, gp100, and tyrosinase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p>Thirty-nine patients with stage IIIB to IV melanoma were vaccinated with this six-peptide mixture weekly at three dose levels, with a preceding phase I dose escalation and subsequent random assignment among the dose levels. Helper T-lymphocyte responses were assessed by in vitro proliferation assay and delayed-type hypersensitivity skin testing. Patients with measurable disease were evaluated for objective clinical response by Response Evaluation Criteria in Solid Tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vaccination with the helper peptide vaccine was well tolerated. Proliferation assays revealed induction of T-cell responses to the melanoma helper peptides in 81% of patients. Among 17 patients with measurable disease, objective clinical responses were observed in two patients (12%), with response durations of 1 and 3.9+ years. Durable stable disease was observed in two additional patients for periods of 1.8 and 4.6+ years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results of this study support the safety and immunogenicity of a vaccine comprised of six melanoma helper peptides. There is also early evidence of clinical activity.<\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2008.17.3161","type":"journal-article","created":{"date-parts":[[2008,9,23]],"date-time":"2008-09-23T00:14:30Z","timestamp":1222128870000},"page":"4973-4980","source":"Crossref","is-referenced-by-count":100,"title":["Helper T-Cell Responses and Clinical Activity of a Melanoma Vaccine With Multiple Peptides From MAGE and Melanocytic Differentiation Antigens"],"prefix":"10.1200","volume":"26","author":[{"suffix":"Jr","given":"Craig L.","family":"Slingluff","sequence":"first","affiliation":[{"name":"From the Department of Surgery\/Division of Surgical Oncology, Department of Public Health Sciences, Cancer Center, Department of Medicine\/Division of Hematology-Oncology, Department of Pathology, and Department of Radiology, University of Virginia Health System, Charlottesville, VA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gina 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