{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:28:11Z","timestamp":1790270891825,"version":"4.1.0"},"reference-count":33,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2009,2,10]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> We recently demonstrated the biologic importance of the nuclear factor kappa B (NF-\u03baB) subunit Rel A in chronic lymphocytic leukemia (CLL) and hypothesized that Rel A DNA binding would have prognostic significance in this disease. <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> Rel A DNA binding was quantified in nuclear extracts derived from 131 unselected CLL patient samples using a quantitative DNA-binding enzyme-linked immunosorbent assay\u2013based method. We then investigated the ability of Rel A to predict for the requirement for treatment and survival and compared our findings with other established prognostic markers. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> Rel A DNA binding was strongly associated with advanced Binet stage (P &lt; .0001) but did not correlate with immunoglobulin V<jats:sub>H<\/jats:sub> (IgV<jats:sub>H<\/jats:sub>) mutation status (P = .25), CD38 expression (P = .87), or zeta-chain\u2013associated protein kinase 70 (ZAP-70) expression (P = .55). It was predictive of time to first treatment (P = .02) and time to subsequent treatment (P = .0001). In addition, Rel A was the most predictive marker of survival both from date of diagnosis (hazard ratio [HR], 9.1; P = .01) and date of entry into the study (HR, 3.9; P = .05) and retained prognostic significance in multivariate analysis for both time to first treatment and overall survival in the presence of Binet stage, IgV<jats:sub>H<\/jats:sub> mutation status, CD38, and ZAP-70. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> Rel A is an independent prognostic marker of survival in CLL and seems to have the unique capacity to predict the duration of response to therapy. Prospective assessment of Rel A as a marker of clinical outcome and as a therapeutic target are now warranted. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2008.19.1114","type":"journal-article","created":{"date-parts":[[2009,1,6]],"date-time":"2009-01-06T01:55:15Z","timestamp":1231206915000},"page":"763-769","source":"Crossref","is-referenced-by-count":48,"title":["Rel A Is an Independent Biomarker of Clinical Outcome in Chronic Lymphocytic Leukemia"],"prefix":"10.1200","volume":"27","author":[{"given":"Saman","family":"Hewamana","sequence":"first","affiliation":[{"name":"From the Department of Haematology; Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff; and the Department of Haematology, Birmingham Heartlands Hospital, Birmingham, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thet Thet","family":"Lin","sequence":"additional","affiliation":[{"name":"From the Department of Haematology; Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff; and the Department of Haematology, Birmingham Heartlands Hospital, Birmingham, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Rowntree","sequence":"additional","affiliation":[{"name":"From the Department of Haematology; Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff; and the Department of Haematology, Birmingham Heartlands Hospital, Birmingham, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamaraj","family":"Karunanithi","sequence":"additional","affiliation":[{"name":"From the Department of Haematology; Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff; and the Department of Haematology, Birmingham Heartlands Hospital, Birmingham, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Pratt","sequence":"additional","affiliation":[{"name":"From the Department of Haematology; 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