{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:05:38Z","timestamp":1793106338993,"version":"4.5.0"},"reference-count":42,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2010,3,20]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> To unravel the regulatory network underlying nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) \u2013mediated lymphomagenesis of anaplastic large-cell lymphoma (ALCL) and to discover diagnostic genomic classifiers for the recognition of patients with ALK-positive and ALK-negative ALCL among T-cell non-Hodgkin's lymphoma (T-NHL). <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> The transcriptome of NPM-ALK\u2013positive ALCL cell lines was characterized by silencing the expression of ALK or STAT3, a major effector of ALK oncogenic activity. Gene expression profiling (GEP) was performed in a series of systemic primary T-NHL (n = 70), including a set of ALK-positive and ALK-negative ALCL (n = 36). Genomic classifiers for ALK-positive and ALK-negative ALCL were generated by prediction analyses and validated by quantitative reverse-transcriptase polymerase chain reaction and\/or immunohistochemistry. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> In ALCL cell lines, two thirds of ALK-regulated genes were concordantly dependent on STAT3 expression. GEP of systemic primary T-NHL significantly clustered ALK-positive ALCL samples in a separate subgroup, underscoring the relevance of in vitro ALK\/STAT3 signatures. A set of genomic classifiers for ALK-positive ALCL and for ALCL were identified by prediction analyses. These gene clusters were instrumental for the distinction of ALK-negative ALCL from peripheral T-cell lymphomas not otherwise specified (PTCLs-NOS) and angioimmunoblastic lymphomas. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> We proved that experimentally controlled GEP in ALCL cell lines represents a powerful tool to identify meaningful signaling networks for the recognition of systemic primary T-NHL. The identification of a molecular signature specific for ALCL suggests that these T-NHLs may represent a unique entity discernible from other PTCLs, and that a restricted number of genes can be instrumental for clinical stratification and, possibly, therapy of T-NHL. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2008.20.9759","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T05:10:00Z","timestamp":1266383400000},"page":"1583-1590","source":"Crossref","is-referenced-by-count":149,"title":["Gene Expression Profiling Uncovers Molecular Classifiers for the Recognition of Anaplastic Large-Cell Lymphoma Within Peripheral T-Cell Neoplasms"],"prefix":"10.1200","volume":"28","author":[{"given":"Roberto","family":"Piva","sequence":"first","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, Verona;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Agnelli","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, Verona;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Pellegrino","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, Verona;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia","family":"Todoerti","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, Verona;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Grosso","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, 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Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, Verona;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Martinoglio","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Center for Experimental Research and Medical Studies, Laboratory of Functional Genomics, and Institute for Cancer Research and Treatment, University of Torino, Torino; Department of Medical Sciences, Leukemia Study Center, University of Milan, Hematology 1, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico and Pathology & Lymphoid Malignancies Units, San Raffaele Scientific Institute, Milan; Department of Pathology, University of Verona, 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