{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:56:09Z","timestamp":1789012569067,"version":"build-2803163510"},"reference-count":21,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2011,5,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> To study minimal disseminated disease (MDD) in children with Burkitt's lymphoma (BL) and to determine its impact on prognosis. <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> We established a simplified long-distance polymerase chain reaction (LD-PCR) assay that can amplify up to 15 to 20 Kb of DNA sequence, making it possible to detect the t(8;14) at the genomic level with sensitivity of 10<jats:sup>\u22124<\/jats:sup>. We prospectively studied diagnostic biopsies and bone marrow aspirates from 134 patients affected by BL. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> A specific LD-PCR product was detected in 96 (72%) of 134 BL biopsies. Among 84 patients with t(8;14) positivity on tumor biopsy and bone marrow (BM), 26 (31%) had LD-PCR\u2013positive BM, and 15 (18%) were positive at standard morphologic analysis. Twenty (85%) of 26 MDD-positive patients belonged to the R4 risk group, according to Berlin-Frankfurt-Munster definition. The 3-year progression-free survival was 68% (SE, 10%) in MDD-positive patients in R4 compared with 93% (SE, 5%) in MDD-negative patients in R4 (P = .03). By multivariate analysis (including MDD, sex, lactate dehydrogenase, CNS involvement), only MDD was predictive of higher risk of failure (hazard ratio, 4.7; P = .04). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> MDD identifies a poor-prognosis subgroup among children with high-risk BL. To improve disease control in these patients, a more effective risk-adapted therapy, possibly including anti-CD20 monoclonal antibody, should be considered. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2010.32.8161","type":"journal-article","created":{"date-parts":[[2011,3,22]],"date-time":"2011-03-22T05:45:42Z","timestamp":1300772742000},"page":"1779-1784","source":"Crossref","is-referenced-by-count":44,"title":["Minimal Disseminated Disease in High-Risk Burkitt's Lymphoma Identifies Patients With Different Prognosis"],"prefix":"10.1200","volume":"29","author":[{"given":"Lara","family":"Mussolin","sequence":"first","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Pillon","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele S.G.","family":"d'Amore","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentino","family":"Conter","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Piglione","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca Lo","family":"Nigro","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Garaventa","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Buffardi","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. Gaslini, Genova; Ospedale Pausilipon, Napoli; A. Ospedaliero, Universitaria Meyer, Firenze; Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Aric\u00f2","sequence":"additional","affiliation":[{"name":"From the Azienda Ospedaliera, Universit\u00e0 di Padova, Padova; Ospedali Riuniti, Bergamo; Ospedale Regina Margherita, Torino; Centro di Riferimento Regionale di Ematologia ed Oncologia Pediatrica, Azienda Policlinico, Ospedaliero-Universitaria Catania; Istituto G. 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