{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:31:24Z","timestamp":1791311484669,"version":"4.3.1"},"reference-count":39,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> Reactivation of Epstein-Barr virus (EBV) after allogeneic stem-cell transplantation (SCT) can lead to severe life-threatening infections and trigger post-transplantation lymphoproliferative disease (PTLD). Since EBV-specific T cells could prevent PTLD, cellular immunotherapy has been a promising treatment option. However, generation of antigen-specific T-cell populations has been difficult within a short time frame. <\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p> To improve availability in urgent clinical conditions, we developed a rapid protocol for isolation of polyclonal EBV nuclear antigen 1 (EBNA-1) \u2013specific T cells by using an interferon gamma (IFN-\u03b3) capture technique. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> We report on the use of adoptive transfer of EBNA-1\u2013specific T cells in 10 pediatric and adult patients with EBV viremia and\/or PTLD after SCT. No acute toxicity or graft-versus-host disease (GVHD) of more than grade 2 occurred as a result of adoptive T-cell transfer. In vivo expansion of transferred EBNA-1\u2013specific T cells was observed in eight of 10 patients after a median of 16 days following adoptive transfer that was associated with clinical and virologic response in seven of them (70%). None of the responders had EBV-associated mortality. Within clinical responders, three patients were disease free by the day of last follow-up (2 to 36 months), three patients died of other infectious complications, and one patient died as a result of relapse of malignancy. EBV-related mortality was observed in two of 10 patients, and another patient had ongoing viremia without clinical symptoms at last follow-up. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> Adoptive ex vivo transfer of EBNA-1\u2013specific T cells is a feasible and well-tolerated therapeutic option, representing a fast and efficient procedure to achieve reconstitution of antiviral T-cell immunity after SCT. <\/jats:p><\/jats:sec>","DOI":"10.1200\/jco.2011.39.8495","type":"journal-article","created":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T06:36:18Z","timestamp":1353393378000},"page":"39-48","source":"Crossref","is-referenced-by-count":256,"title":["Adoptive Transfer of Epstein-Barr Virus (EBV) Nuclear Antigen 1\u2013Specific T Cells As Treatment for EBV Reactivation and Lymphoproliferative Disorders After Allogeneic Stem-Cell Transplantation"],"prefix":"10.1200","volume":"31","author":[{"given":"Vanya","family":"Icheva","sequence":"first","affiliation":[{"name":"Vanya Icheva, Simone Kayser, Michael Schumm, Rupert Handgretinger, Peter Lang, and Tobias Feuchtinger, University Children's Hospital T\u00fcbingen; Christina Kyzirakos and Stefan Stevanovic, University of T\u00fcbingen; Wolfgang Bethge, University Hospital T\u00fcbingen, T\u00fcbingen; Daniel Wolff, University Hospital Regensburg, Regensburg; Sebastian Tuve, University Hospital Dresden, Dresden; Johann Greil, University Children's Hospital Heidelberg, Heidelberg; Michael H. Albert, Haunersches Children's Hospital M\u00fcnchen;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Kayser","sequence":"additional","affiliation":[{"name":"Vanya Icheva, Simone Kayser, Michael Schumm, Rupert Handgretinger, Peter Lang, and Tobias Feuchtinger, University Children's Hospital T\u00fcbingen; Christina Kyzirakos and Stefan Stevanovic, University of T\u00fcbingen; Wolfgang Bethge, University Hospital T\u00fcbingen, T\u00fcbingen; Daniel Wolff, University Hospital Regensburg, Regensburg; Sebastian Tuve, University Hospital Dresden, Dresden; Johann Greil, University Children's Hospital Heidelberg, Heidelberg; Michael H. 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