{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T23:19:33Z","timestamp":1791674373218,"version":"4.3.4"},"reference-count":29,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCO"],"published-print":{"date-parts":[[2014,6,10]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Carcinomas of the anal canal are strongly associated with the human papillomavirus (HPV). Expression of p16 is used as a surrogate marker of HPV infection. In a retrospective study, we evaluated HPV genotyping and p16 expression as prognostic markers of overall survival (OS) and disease-specific survival (DSS) in patients diagnosed with American Joint Committee on Cancer (AJCC) stages I to III carcinoma of the anal canal.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>HPV genotyping polymerase chain reaction (high-risk subtypes 16, 18, 31, 33, 45, 52, and 58) and immunohistochemical expression of p16 were analyzed by using paraffin-embedded tumor biopsies from 143 anal carcinomas. The patients were treated with combined chemoradiotherapy or radiotherapy alone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HPV16 was detected in 81.0% of the tumors, followed by HPV33 (5.1%), HPV18 (2.2%), and HPV58 (0.7%). p16 positivity was found in 92.9% of the tumors. In univariable survival analysis, HPV positivity was significantly correlated with improved OS (74% v 52%; P = .036) and DSS (84% v 52%; P = .002), and p16 positivity was significantly correlated with improved OS (76% v 30%; P &lt; .001) and DSS (85% v 30%; P &lt; .001). In multivariable COX analysis that included HPV status, p16 status, sex, T stage, N stage, and treatment, p16 positivity remained an independent prognostic factor for OS (hazard ratio [HR], 0.07; 95% CI, 0.01 to 0.61; P = .016) and DSS (HR, 0.07; 95% CI, 0.01 to 0.53; P = .011).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>p16 positivity is an independent prognostic factor for OS and DSS in patients with AJCC stages I to III carcinoma of the anal canal.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1200\/jco.2013.52.3464","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T04:46:29Z","timestamp":1399956389000},"page":"1812-1817","source":"Crossref","is-referenced-by-count":168,"title":["Human Papillomavirus Genotyping and p16 Expression As Prognostic Factors for Patients With American Joint Committee on Cancer Stages I to III Carcinoma of the Anal Canal"],"prefix":"10.1200","volume":"32","author":[{"given":"Eva","family":"Serup-Hansen","sequence":"first","affiliation":[{"name":"All authors: Copenhagen University Hospital Herlev, Herlev, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte","family":"Linnemann","sequence":"additional","affiliation":[{"name":"All authors: Copenhagen University Hospital Herlev, Herlev, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Skovrider-Ruminski","sequence":"additional","affiliation":[{"name":"All authors: Copenhagen University Hospital Herlev, Herlev, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estrid","family":"H\u00f8gdall","sequence":"additional","affiliation":[{"name":"All authors: Copenhagen University Hospital Herlev, Herlev, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poul 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