{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:51:05Z","timestamp":1781815865751,"version":"3.54.5"},"reference-count":35,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"6","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Precis Oncol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>PURPOSE<\/jats:title>\n                    <jats:p>\n                      To address the growing incidence of endometrial cancer (EC), noninvasive detection methods are needed. Although self-sampling is proven for cervical cancer screening, its utility for EC detection via methylation analysis lacks robust evidence. This study aimed to validate this approach by evaluating the concordance and diagnostic accuracy of\n                      <jats:italic toggle=\"yes\">CDO1<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">CELF4<\/jats:italic>\n                      methylation testing in self-collected samples compared with clinician-collected samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      In this prospective cross-sectional study, paired self-collected (Evalyn Brush) and physician-collected cervical samples were obtained from women referred for hysteroscopy.\n                      <jats:italic toggle=\"yes\">CDO1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">CELF4<\/jats:italic>\n                      methylation levels were measured by qPCR. Method agreement was analyzed with Spearman correlation and Bland-Altman plots, and diagnostic performance was evaluated by the area under the ROC curve (AUC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Analysis of 142 participants revealed high agreement between self- and physician-collected samples, with significant correlations (\n                      <jats:italic toggle=\"yes\">CDO1<\/jats:italic>\n                      R = 0.79,\n                      <jats:italic toggle=\"yes\">CELF4<\/jats:italic>\n                      R = 0.76;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; .05) and Bland-Altman analysis confirming minimal bias (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &gt; .05 for both genes). Self and physician sampling showed comparable efficacy for EC detection via\n                      <jats:italic toggle=\"yes\">CDO1<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">CELF4<\/jats:italic>\n                      methylation, with AUCs of 0.930 and 0.896, respectively. Methylation levels (\u0394Ct) differed significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; .001) between EC (n = 33) and non-EC (n = 109) groups, regardless of the collection method. The diagnostic performance remained consistently high across premenopausal and postmenopausal subgroups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Self-collected vaginal samples for\n                      <jats:italic toggle=\"yes\">CDO1<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">CELF4<\/jats:italic>\n                      methylation analysis demonstrate high agreement and diagnostic accuracy equivalent to physician-collected samples for EC detection. This self-sampling strategy represents a reliable, noninvasive, and patient-centric approach, showing potential for integration into future EC diagnosis strategies to improve accessibility and early detection. Future multicenter studies are warranted to validate its effectiveness in broader populations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1200\/po-25-01081","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:59:13Z","timestamp":1781812753000},"update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Accuracy and Concordance of Self-Collected Cervical CDO1 and CELF4 Methylation Testing Compared With Physician Sampling for Endometrial Cancer Detection"],"prefix":"10.1200","volume":"10","author":[{"given":"Peixuan","family":"He","sequence":"first","affiliation":[{"name":"Department of Gynecology, The Second Xiangya Hospital of Central South University, Changsha, China"},{"name":"Clinical Research Center for Gynecological Disease in Hunan Province, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9460-3608","authenticated-orcid":true,"given":"Yishan","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Origin-Poly Bio-Tec Co, Ltd, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Gynecology, The Second Xiangya Hospital of Central South University, Changsha, China"},{"name":"Clinical Research Center for Gynecological Disease in Hunan Province, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengniang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gynecology, The Second Xiangya Hospital of Central South University, Changsha, China"},{"name":"Clinical Research Center for Gynecological Disease in Hunan Province, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Origin-Poly Bio-Tec Co, Ltd, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhai","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Origin-Poly Bio-Tec Co, Ltd, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyue","family":"Yin","sequence":"additional","affiliation":[{"name":"Beijing Origin-Poly Bio-Tec Co, Ltd, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunxian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gynecology, The Second Xiangya Hospital of Central South University, Changsha, China"},{"name":"Clinical Research Center for Gynecological Disease in Hunan Province, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyi","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Harbin Medical University, Harbin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-1159","authenticated-orcid":true,"given":"Chun","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Gynecology, The Second Xiangya Hospital of Central South University, Changsha, China"},{"name":"Clinical Research Center for Gynecological Disease in Hunan Province, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"233","reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2021.01.036"},{"key":"e_1_3_8_3_2","volume-title":"Survival for Womb Cancer","author":"Cancer Search UK","unstructured":"Cancer Search UK: Survival for Womb Cancer: https:\/\/www.cancerresearchuk.org\/about-cancer\/womb-cancer\/survival"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2024.06.026"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16050907"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2008.01773.x"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004952"},{"key":"e_1_3_8_8_2","first-page":"195","article-title":"Quantitative assessment of DNA methylation for the detection of cervical and endometrial adenocarcinomas in liquid-based cytology specimens","volume":"34","author":"Kim G-E","year":"2012","unstructured":"Kim G-E, Kweon S-S, Lee JS, et al: Quantitative assessment of DNA methylation for the detection of cervical and endometrial adenocarcinomas in liquid-based cytology specimens. 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