{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:17:59Z","timestamp":1774473479011,"version":"3.50.1"},"reference-count":36,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"3","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Precis Oncol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>PURPOSE<\/jats:title>\n                    <jats:p>Gastroesophageal carcinoma (GEC) is increasingly managed using biomarker-guided therapies. Although both tissue DNA (tDNA) and circulating tumor DNA (ctDNA) testing are used clinically, the concordance of genomic alterations between primary tumors, metastatic sites, and liquid biopsy remains incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      We retrospectively analyzed 345 patients with GEC who underwent ctDNA testing at Mayo Clinic. tDNA next-generation sequencing and immunohistochemistry (IHC) for human epidermal growth factor receptor 2 (HER2)\/erb-b2 receptor tyrosine kinase 2 (\n                      <jats:italic toggle=\"yes\">ERBB2<\/jats:italic>\n                      ) and mismatch repair (MMR) were collected. Concordance was evaluated between ctDNA and tDNA, ctDNA and IHC, and across all three modalities, using 60-day and 30-day intervals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Among 345 patients with ctDNA testing, 186 had tDNA testing, and 310 underwent IHC. ctDNA testing showed a shorter turnaround time than tDNA (median 6\n                      <jats:italic toggle=\"yes\">v<\/jats:italic>\n                      13 days). The most frequent alteration in ctDNA, primary tDNA, and metastatic tDNA was\n                      <jats:italic toggle=\"yes\">TP53.<\/jats:italic>\n                      Concordance between ctDNA and primary tDNA was \u226578.8% for key genes; between ctDNA and metastatic tDNA, it was \u226584.8%. IHC-ctDNA concordance was higher in metastatic tumors, with 91.1% for HER2\/\n                      <jats:italic toggle=\"yes\">ERBB2<\/jats:italic>\n                      and 97.7% for mismatch repair (MMR)\/microsatellite instability (MSI) compared with 81.1% and 95.8%, respectively, in primary tumors. Triplet concordance for MSI\/MMR was 97.6% in primary tumors and 100% in metastatic tumors, while\n                      <jats:italic toggle=\"yes\">ERBB2<\/jats:italic>\n                      \/HER2 showed 84.4% and 93.9% concordance, respectively. ctDNA temporal heterogeneity analysis revealed 14 patients with fluctuating\n                      <jats:italic toggle=\"yes\">ERBB2<\/jats:italic>\n                      status and 3 with fluctuating MSI-H status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>ctDNA testing demonstrates high concordance with tDNA and IHC and offers faster turnaround. Given its minimally invasive nature and strong concordance with tissue-based testing, ctDNA is a reliable tool for genomic profiling and longitudinal monitoring in GEC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1200\/po-25-01194","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:59:09Z","timestamp":1774468749000},"update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Concordance of Circulating Tumor DNA, Primary and Metastatic Tissue DNA, and Immunohistochemistry in Gastroesophageal Carcinoma"],"prefix":"10.1200","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6286-3855","authenticated-orcid":true,"given":"Fares","family":"Jamal","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology, Comprehensive Cancer Center, Mayo Clinic, Phoenix, 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