{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:44:28Z","timestamp":1782938668820,"version":"3.54.5"},"reference-count":33,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"7","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Precis Oncol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>PURPOSE<\/jats:title>\n                    <jats:p>Nasopharyngeal carcinoma (NPC) is a multifactorial malignancy often diagnosed at an advanced stage due to nonspecific early symptoms. Accurate prognostic stratification is essential for individualized therapy but remains challenging because of biological and clinical heterogeneity. This study aimed to develop and validate a gene expression\u2013based prognostic signature for locally advanced NPC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>This retrospective biomarker investigation combined transcriptomic profiling and survival analyses. A prognostic model was constructed using LASSO-Cox regression in the discovery cohort (N = 99) from the multicenter, randomized phase III trial NPC-0501, and validated in independent cohorts (N = 133) from Queen Elizabeth Hospital (QEH), Hong Kong, and Sun Yat-sen University Cancer Center (SYS), Guangzhou, using different profiling methods. Mechanisms underlying the signature were explored using single-cell RNA-seq (scRNA-seq) data. The primary outcome was 3-year progression-free survival (PFS), with 5-year overall survival (OS) as a secondary end point. Performance was evaluated using hazard ratios (HRs) and survival probabilities (SP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A 5-gene signature (\n                      <jats:italic toggle=\"yes\">SPP1<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">IL18BP<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">PALMD<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">WDR35<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">SOCS6<\/jats:italic>\n                      ) predicted 3-year PFS in the NPC-0501 cohort (HR, 0.046 [95% CI, 0.011 to 0.190];\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; .001), separating high-risk from low-risk patients (SP, 42.9%\n                      <jats:italic toggle=\"yes\">v<\/jats:italic>\n                      96.0%;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; .0001). It predicted survival in both validation cohorts, with associations with 5-year OS in QEH (SP, 72.7%\n                      <jats:italic toggle=\"yes\">v<\/jats:italic>\n                      100%;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; .001) and 3-year PFS in SYS (SP, 70.5%\n                      <jats:italic toggle=\"yes\">v<\/jats:italic>\n                      93.2%;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .0059). It outperformed a published metastasis-related model. Single-cell analyses showed\n                      <jats:italic toggle=\"yes\">SPP1<\/jats:italic>\n                      enrichment in M2 macrophages, linking high risk with an immunosuppressive tumor microenvironment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>A robust five-gene signature was established and validated for prognostic stratification of locally advanced NPC. Reproducibility across transcriptomic platforms and biological relevance support clinical application to guide personalized treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1200\/po-26-00029","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:59:11Z","timestamp":1782935951000},"update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Cross-Platform Gene Signature to Predict Survival Outcomes for Nasopharyngeal Carcinoma"],"prefix":"10.1200","volume":"10","author":[{"given":"Huaping","family":"Li","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyu","family":"Jing","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"},{"name":"Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James Chung Hang","family":"Chow","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wah","family":"Cheuk","sequence":"additional","affiliation":[{"name":"Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0821-6397","authenticated-orcid":true,"given":"Kenneth Sik Kwan","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2736-4379","authenticated-orcid":true,"given":"Suyu","family":"Hao","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5457-0648","authenticated-orcid":true,"given":"Kazi Anisha","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5558-0685","authenticated-orcid":true,"given":"Wei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7523-5088","authenticated-orcid":false,"given":"Ka On","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6283-978X","authenticated-orcid":true,"given":"Victor Ho Fun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Tong","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong 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