{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:51:15Z","timestamp":1753883475006,"version":"3.41.2"},"reference-count":31,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"8","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Precis Oncol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>PURPOSE<\/jats:title><jats:p> Cancer patients with advanced-stage disease have poor prognosis, typically having limited options for efficacious treatment, and genomics-based therapy guidance continues to benefit only a fraction of patients. Next-generation ex vivo approaches, such as cell mass-based response testing (MRT), offer an alternative precision medicine approach for a broader population of patients with cancer, but validation of clinical feasibility and potential impact remain necessary. <\/jats:p><\/jats:sec><jats:sec><jats:title>MATERIALS AND METHODS<\/jats:title><jats:p> We evaluated the clinical feasibility and accuracy of using live-cell MRT to predict patient drug sensitivity. Using a unified measurement workflow with a 48-hour result turnaround time, samples were subjected to MRT after treatment with a panel of drugs in vitro. After completion of therapeutic course, clinical response data were correlated with MRT-based predictions of outcome. Specimens were collected from 104 patients with solid (n = 69) and hematologic (n = 35) malignancies, using tissue formats including needle biopsies, malignant fluids, bone marrow aspirates, and blood samples. Of the 81 (78%) specimens qualified for MRT, 41 (51%) patients receiving physician-selected therapies had treatments matched to MRT. <\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p> MRT demonstrated high concordance with clinical responses with an odds ratio (OR) of 14.80 ( P = .0003 [95% CI, 2.83 to 102.9]). This performance held for both solid and hematologic malignances with ORs of 20.67 ( P = .0128 [95% CI, 1.45 to 1,375.57]) and 8.20 ( P = .045 [95% CI, 0.77 to 133.56]), respectively. Overall, these results had a predictive accuracy of 80% ( P = .0026 [95% CI, 65 to 91]). <\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p> MRT showed highly significant correlation with clinical response to therapy. Routine clinical use is technically feasible and broadly applicable to a wide range of samples and malignancy types, supporting the need for future validation studies. <\/jats:p><\/jats:sec>","DOI":"10.1200\/po.23.00349","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T20:59:22Z","timestamp":1705611562000},"update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":2,"title":["Cellular Mass Response to Therapy Correlates With Clinical Response for a Range of Malignancies"],"prefix":"10.1200","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9207-4979","authenticated-orcid":true,"given":"Mark M.","family":"Stevens","sequence":"first","affiliation":[{"name":"Travera, Medford, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Kimmerling","sequence":"additional","affiliation":[{"name":"Travera, Medford, 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