{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T19:48:44Z","timestamp":1774554524848,"version":"3.50.1"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AAPS PharmSci"],"published-print":{"date-parts":[[2000,3]]},"DOI":"10.1208\/ps020102","type":"journal-article","created":{"date-parts":[[2003,8,8]],"date-time":"2003-08-08T10:41:42Z","timestamp":1060339302000},"page":"9-21","source":"Crossref","is-referenced-by-count":12,"title":["Molecular modeling of G-protein coupled receptor kinase 2: Docking and biochemical evaluation of inhibitors"],"prefix":"10.1208","volume":"2","author":[{"given":"Matthias U.","family":"Kassack","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"H\u00f6gger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Gschwend","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimihiko","family":"Kameyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Haga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Graul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Sad\u00e9e","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2000,3,1]]},"reference":[{"key":"210009_CR1","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0092-8674(93)80042-D","volume":"74","author":"RJ Lefkowitz","year":"1993","unstructured":"Lefkowitz RJ. G protein-coupled receptor kinases. Cell. 1993;74:409\u2013412.","journal-title":"Cell"},{"key":"210009_CR2","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1096\/fasebj.4.11.2165947","volume":"4","author":"WP Hausdorff","year":"1990","unstructured":"Hausdorff WP, Caron MG, Lefkowitz RJ. Turning off the signal: Desensitization of beta-adrenergic receptor function [published erratum appears in FASEB J 1990 Sep; 4(12):3049]. FASEB J. 1990;4:2881\u20132889.","journal-title":"FASEB J"},{"key":"210009_CR3","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/0968-0004(91)90157-Q","volume":"16","author":"K Palczewski","year":"1991","unstructured":"Palczewski K, Benovic JL. G-protein-coupled receptor kinases. Trends Biochem Sci. 1991;16:387\u2013391.","journal-title":"Trends Biochem Sci"},{"key":"210009_CR4","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1046\/j.1471-4159.1994.63020400.x","volume":"63","author":"T Haga","year":"1994","unstructured":"Haga T, Haga K, Kameyama K. G protein-coupled receptor kinases. J Neurochem. 1994;63:400\u2013412.","journal-title":"J Neurochem"},{"key":"210009_CR5","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1146\/annurev.biochem.67.1.653","volume":"67","author":"JA Pitcher","year":"1998","unstructured":"Pitcher JA, Freedman NJ, Lefkowitz RJ. G protein-coupled receptor kinases. Annu Rev Biochem. 1998;67:653\u2013692.","journal-title":"Annu Rev Biochem"},{"key":"210009_CR6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1126\/science.2552582","volume":"246","author":"JL Benovic","year":"1989","unstructured":"Benovic JL, DeBlasi A, Stone WC, Caron MG, Lefkowitz RJ. Beta-adrenergic receptor kinase: Primary structure delineates a multigene family. Science. 1989;246:235\u2013240.","journal-title":"Science"},{"key":"210009_CR7","doi-asserted-by":"crossref","first-page":"17251","DOI":"10.1016\/S0021-9258(18)45367-7","volume":"262","author":"JL Benovic","year":"1987","unstructured":"Benovic JL, Regan JW, Matsui H, et al. Agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase. J Biol Chem. 1987;262:17251\u201317253.","journal-title":"J Biol Chem"},{"key":"210009_CR8","doi-asserted-by":"crossref","first-page":"4543","DOI":"10.1021\/bi00437a005","volume":"28","author":"MM Kwatra","year":"1989","unstructured":"Kwatra MM, Benovic JL, Caron MG, Lefkowitz RJ, Hosey MM. Phosphorylation of chick heart muscarinic cholinergic receptors by the beta-adrenergic receptor kinase. Biochemistry. 1989;28:4543\u20134547.","journal-title":"Biochemistry"},{"key":"210009_CR9","doi-asserted-by":"crossref","first-page":"2776","DOI":"10.1074\/jbc.271.5.2776","volume":"271","author":"K Haga","year":"1996","unstructured":"Haga K, Kameyama K, Haga T, Kikkawa U, Shiozaki K, Uchiyama H. Phosphorylation of human m1 muscarinic acetylcholine receptors by G protein-coupled receptor kinase 2 and protein kinase C. J Biol Chem. 1996;271:2776\u20132782.","journal-title":"J Biol Chem"},{"key":"210009_CR10","first-page":"173","volume":"48","author":"G Pei","year":"1995","unstructured":"Pei G, Kieffer BL, Lefkowitz RJ, Freedman NJ. Agonist-dependent phosphorylation of the mouse delta-opioid receptor: Involvement of G protein-coupled receptor kinases but not protein kinase C. Mol Pharmacol. 1995;48:173\u2013177.","journal-title":"Mol Pharmacol"},{"key":"210009_CR11","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/321869a0","volume":"321","author":"JL Benovic","year":"1986","unstructured":"Benovic JL, Mayor FJ, Somers RL, Caron MG, Lefkowitz RJ. Light-dependent phosphorylation of rhodopsin by beta-adrenergic receptor kinase. Nature. 1986;321:869\u2013872.","journal-title":"Nature"},{"key":"210009_CR12","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0076-6879(91)00152-M","volume":"200","author":"JL Benovic","year":"1991","unstructured":"Benovic JL. Purification and characterization of beta-adrenergic receptor kinase. Methods Enzymol. 1991;200:351\u2013362.","journal-title":"Methods Enzymol"},{"key":"210009_CR13","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1126\/science.1862342","volume":"253","author":"DR Knighton","year":"1991","unstructured":"Knighton DR, Zheng JH, Ten Eyck LF, et al. Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase [see comments]. Science. 1991;253:407\u2013414.","journal-title":"Science"},{"key":"210009_CR14","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1021\/bi00060a005","volume":"32","author":"J Zheng","year":"1993","unstructured":"Zheng J, Knighton DR, Ten Eyck LF, Karlsson R, Xuong N, Taylor SS, Sowadski JM. Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor. Biochemistry. 1993;32:2154\u20132161.","journal-title":"Biochemistry"},{"key":"210009_CR15","first-page":"145","volume":"14","author":"M Iino","year":"1996","unstructured":"Iino M, Shibano T. Substrate recognition mechanism of human beta-adrenergic receptor kinase 1 based on a 2-dimensional model structure. Drug Des Discov. 1996;14:145\u2013155.","journal-title":"Drug Des Discov"},{"key":"210009_CR16","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0022-2836(82)90153-X","volume":"161","author":"ID Kuntz","year":"1982","unstructured":"Kuntz ID, Blaney JM, Oatley SJ, Langridge R, Ferrin TE. A geometric approach to macromolecule-ligand interactions. J Mol Biol. 1982;161:269\u2013288.","journal-title":"J Mol Biol"},{"key":"210009_CR17","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1002\/jcc.540130311","volume":"13","author":"BK Shoichet","year":"1992","unstructured":"Shoichet BK, Bodian DL, Kuntz ID. Molecular docking using shape descriptors. J Comp Chem. 1992;13:380\u2013397.","journal-title":"J Comp Chem"},{"key":"210009_CR18","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/jcc.540130412","volume":"13","author":"EC Meng","year":"1992","unstructured":"Meng EC, Shoichet BK, Kuntz ID. Automated docking with grid-based energy evaluation. J Comp Chem. 1992;13:505\u2013524.","journal-title":"J Comp Chem"},{"key":"210009_CR19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00402820","volume":"10","author":"DA Gschwend","year":"1996","unstructured":"Gschwend DA, Kuntz ID. Orientational sampling and rigid-body minimization in molecular docking revisited: On-the-fly optimization and degeneracy removal. J Comput Aided Mol Des. 1996;10:123\u2013132.","journal-title":"J Comput Aided Mol Des"},{"key":"210009_CR20","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1126\/science.257.5073.1078","volume":"257","author":"ID Kuntz","year":"1992","unstructured":"Kuntz ID. Structure-based strategies for drug design and discovery [see comments]. Science. 1992;257:1078\u20131082.","journal-title":"Science"},{"key":"210009_CR21","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1002\/prot.340090107","volume":"9","author":"C Sander","year":"1991","unstructured":"Sander C, Schneider R. Database of homology-derived protein structures and the structural meaning of sequence alignment. Proteins. 1991;9:56\u201368.","journal-title":"Proteins"},{"key":"210009_CR22","doi-asserted-by":"crossref","first-page":"7558","DOI":"10.1073\/pnas.90.16.7558","volume":"90","author":"B Rost","year":"1993","unstructured":"Rost B, Sander C. Improved prediction of protein secondary structure by use of sequence profiles and neural ne2rks. Proc Natl Acad Sci USA. 1993;90:7558\u20137562.","journal-title":"Proc Natl Acad Sci USA"},{"key":"210009_CR23","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1006\/jmbi.1993.1413","volume":"232","author":"B Rost","year":"1993","unstructured":"Rost B, Sander C. Prediction of protein secondary structure at better than 70% accuracy. J Mol Biol. 1993;232:584\u2013599.","journal-title":"J Mol Biol"},{"key":"210009_CR24","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0022-2836(77)80200-3","volume":"112","author":"FC Bernstein","year":"1977","unstructured":"Bernstein FC, Koetzle TF, Williams GJ, et al. The Protein Data Bank: A computer-based archival file for macromolecular structures. J Mol Biol. 1977;112:535\u2013542.","journal-title":"J Mol Biol"},{"key":"210009_CR25","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0263-7855(88)80054-7","volume":"6","author":"TE Ferrin","year":"1988","unstructured":"Ferrin TE, Huang CC, Jarvis LE, Langridge R. The MIDAS Display System. J. Mol. Graph. 1988;6:13\u201327.","journal-title":"J. Mol. Graph."},{"key":"210009_CR26","unstructured":"Sybyl v6.04a. Tripos Associates Inc., St. Louis, MO."},{"key":"210009_CR27","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1126\/science.6879170","volume":"221","author":"ML Connolly","year":"1983","unstructured":"Connolly ML. Solvent-accessible surfaces of proteins and nucleic acids. Science. 1983;221:709\u2013713.","journal-title":"Science"},{"key":"210009_CR28","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1126\/science.1862343","volume":"253","author":"DR Knighton","year":"1991","unstructured":"Knighton DR, Zheng JH, Ten Eyck LF, Xuong NH, Taylor SS, Sowadski JM. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase [see comments]. Science. 1991;253:414\u2013420.","journal-title":"Science"},{"key":"210009_CR29","unstructured":"MDL Information Systems Inc., San Leandro, CA."},{"key":"210009_CR30","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/BF00126218","volume":"6","author":"GW Bemis","year":"1992","unstructured":"Bemis GW, Kuntz ID. A fast and efficient method for 2D and 3D molecular shape description. J Comput Aided Mol Des. 1992;6:607\u2013628.","journal-title":"J Comput Aided Mol Des"},{"key":"210009_CR31","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.1016\/S0021-9258(18)53021-0","volume":"268","author":"K Kameyama","year":"1993","unstructured":"Kameyama K, Haga K, Haga T, Kontani K, Katada T, Fukada Y. Activation by G protein beta gamma subunits of beta-adrenergic and muscarinic receptor kinase. J Biol Chem. 1993;268:7753\u20137758.","journal-title":"J Biol Chem"},{"key":"210009_CR32","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1021\/bi00541a032","volume":"21","author":"U Wilden","year":"1982","unstructured":"Wilden U, Kuhn H. Light-dependent phosphorylation of rhodopsin: Number of phosphorylation sites. Biochemistry. 1982;21:3014\u20133022.","journal-title":"Biochemistry"},{"key":"210009_CR33","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1073\/pnas.90.2.442","volume":"90","author":"AC Carrera","year":"1993","unstructured":"Carrera AC, Alexandrov K, Roberts TM. The conserved lysine of the catalytic domain of protein kinases is actively involved in the phosphotransfer reaction and not required for anchoring ATP. Proc Natl Acad Sci U S A. 1993;90:442\u2013446.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"210009_CR34","doi-asserted-by":"crossref","first-page":"5036","DOI":"10.1021\/bi00316a032","volume":"23","author":"H Hidaka","year":"1984","unstructured":"Hidaka H, Inagaki M, Kawamoto S, Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984;23:5036\u20135041.","journal-title":"Biochemistry"},{"key":"210009_CR35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0014-5793(96)00467-X","volume":"387","author":"Z Wang","year":"1996","unstructured":"Wang Z, Arden J, Sadee W. Basal phosphorylation of mu opioid receptor is agonist modulated and Ca2+-dependent. FEBS Lett. 1996;387:53\u201357.","journal-title":"FEBS Lett"},{"key":"210009_CR36","first-page":"177","volume":"45","author":"TE Voogd","year":"1993","unstructured":"Voogd TE, Vansterkenburg EL, Wilting J, Janssen LH. Recent research on the biological activity of suramin. Pharmacol Rev. 1993;45:177\u2013203.","journal-title":"Pharmacol Rev"},{"key":"210009_CR37","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0959-440X(91)90035-R","volume":"1","author":"SK Hanks","year":"1991","unstructured":"Hanks SK. Eukaryotic protein kinases. Curr Opin Struct Biol. 1991;1:369\u2013383.","journal-title":"Curr Opin Struct Biol"},{"key":"210009_CR38","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1073\/pnas.90.8.3583","volume":"90","author":"CS Ring","year":"1993","unstructured":"Ring CS, Sun E, McKerrow JH, Lee GK, Rosenthal PJ, Kuntz ID, Cohen FE. Structure-based inhibitor design by using protein models for the development of antiparasitic agents. Proc Natl Acad Sci U S A. 1993;90:3583\u20133587.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"210009_CR39","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0014-5793(89)81639-4","volume":"258","author":"CE Hensey","year":"1989","unstructured":"Hensey CE, Boscoboinik D, Azzi A. Suramin, an anti-cancer drug, inhibits protein kinase C and induces differentiation in neuroblastoma cell clone NB2A. FEBS Lett. 1989;258:156\u2013158.","journal-title":"FEBS Lett"},{"key":"210009_CR40","doi-asserted-by":"crossref","first-page":"5424","DOI":"10.1016\/S0021-9258(19)39377-9","volume":"265","author":"CW Mahoney","year":"1990","unstructured":"Mahoney CW, Azzi A, Huang KP. Effects of suramin, an antihuman immunodeficiency virus reverse transcriptase agent on protein kinase C: Differential activation and inhibition of protein kinase C isozymes. J Biol Chem. 1990;265:5424\u20135428.","journal-title":"J Biol Chem"},{"key":"210009_CR41","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0304-3835(93)90057-G","volume":"75","author":"S Baghdiguian","year":"1993","unstructured":"Baghdiguian S, Boudier JA, Boudier JL, Fantini J. Autoradiographic localization of tritiated suramin in polarized human colon adenocarcinoma cells. Cancer Lett. 1993;75:151\u2013156.","journal-title":"Cancer Lett"}],"container-title":["AAPS PharmSci"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/ps020102.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1208\/ps020102\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/ps020102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T03:18:44Z","timestamp":1623295124000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1208\/ps020102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["210009"],"URL":"https:\/\/doi.org\/10.1208\/ps020102","relation":{},"ISSN":["1522-1059"],"issn-type":[{"value":"1522-1059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}