{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T19:42:00Z","timestamp":1774554120519,"version":"3.50.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T00:00:00Z","timestamp":1431993600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["AAPS J"],"published-print":{"date-parts":[[2015,9]]},"DOI":"10.1208\/s12248-015-9778-9","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T18:36:40Z","timestamp":1431974200000},"page":"1193-1199","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The Role of Megalin in the Transport of Gentamicin Across BeWo Cells, an In Vitro Model of\u00a0the Human Placenta"],"prefix":"10.1208","volume":"17","author":[{"given":"Amal A.","family":"Akour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Jayne","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9861-1018","authenticated-orcid":false,"given":"Phillip M.","family":"Gerk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,5,19]]},"reference":[{"issue":"4","key":"9778_CR1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0146-0005(98)80013-X","volume":"22","author":"JW Riggs","year":"1998","unstructured":"Riggs JW, Blanco JD. Pathophysiology, diagnosis, and management of intraamniotic infection. Semin Perinatol. 1998;22(4):251\u20139.","journal-title":"Semin Perinatol"},{"issue":"3","key":"9778_CR2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jmwh.2008.01.001","volume":"53","author":"JO Fahey","year":"2008","unstructured":"Fahey JO. Clinical management of intra-amniotic infection and chorioamnionitis: a review of the literature. J Midwifery Womens Health. 2008;53(3):227\u201335.","journal-title":"J Midwifery Womens Health"},{"issue":"2","key":"9778_CR3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.clp.2010.02.003","volume":"37","author":"AT Tita","year":"2010","unstructured":"Tita AT, Andrews WW. Diagnosis and management of clinical chorioamnionitis. Clin Perinatol. 2010;37(2):339\u201354.","journal-title":"Clin Perinatol"},{"key":"9778_CR4","volume":"3","author":"L Hopkins","year":"2002","unstructured":"Hopkins L, Smaill F. Antibiotic regimens for management of intraamniotic infection. Cochrane Database Syst Rev. 2002;3, CD003254.","journal-title":"Cochrane Database Syst Rev"},{"issue":"4","key":"9778_CR5","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1067\/mob.2002.122970","volume":"186","author":"GD Wendel Jr","year":"2002","unstructured":"Wendel Jr GD, Leveno KJ, Sanchez PJ, Jackson GL, McIntire DD, Siegel JD. Prevention of neonatal group B streptococcal disease: a combined intrapartum and neonatal protocol. Am J Obstet Gynecol. 2002;186(4):618\u201326.","journal-title":"Am J Obstet Gynecol"},{"issue":"5","key":"9778_CR6","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1097\/01.AOG.0000216197.26783.b5","volume":"107","author":"GG Nahum","year":"2006","unstructured":"Nahum GG, Uhl K, Kennedy DL. Antibiotic use in pregnancy and lactation: what is and is not known about teratogenic and toxic risks. Obstet Gynecol. 2006;107(5):1120\u201338.","journal-title":"Obstet Gynecol"},{"issue":"4","key":"9778_CR7","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1128\/AAC.11.4.688","volume":"11","author":"B Bernard","year":"1977","unstructured":"Bernard B, Garcia-Cazares SJ, Ballard CA, Thrupp LD, Mathies AW, Wehrle PF. Tobramycin: maternal-fetal pharmacology. Antimicrob Agents Chemother. 1977;11(4):688\u201394.","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"9778_CR8","first-page":"124","volume":"72","author":"LC Gilstrap 3rd","year":"1988","unstructured":"Gilstrap 3rd LC, Bawdon RE, Burris J. Antibiotic concentration in maternal blood, cord blood, and placental membranes in chorioamnionitis. Obstet Gynecol. 1988;72(1):124\u20135.","journal-title":"Obstet Gynecol"},{"issue":"5","key":"9778_CR9","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1128\/AAC.21.5.721","volume":"21","author":"JJ Schentag","year":"1982","unstructured":"Schentag JJ, Cerra FB, Plaut ME. Clinical and pharmacokinetic characteristics of aminoglycoside nephrotoxicity in 201 critically ill patients. Antimicrob Agents Chemother. 1982;21(5):721\u20136.","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"9778_CR10","doi-asserted-by":"crossref","first-page":"F337","DOI":"10.1152\/ajprenal.2001.281.2.F337","volume":"281","author":"J Nagai","year":"2001","unstructured":"Nagai J, Tanaka H, Nakanishi N, Murakami T, Takano M. Role of megalin in renal handling of aminoglycosides. Am J Physiol Renal Physiol. 2001;281(2):F337\u201344.","journal-title":"Am J Physiol Renal Physiol"},{"key":"9778_CR11","doi-asserted-by":"crossref","unstructured":"Watanabe A, Nagai J, Adachi Y, Katsube T, Kitahara Y, Murakami T, et al. Targeted prevention of renal accumulation and toxicity of gentamicin by aminoglycoside binding receptor antagonists. J Control Release. 2004;95(3):423\u201333.","DOI":"10.1016\/j.jconrel.2003.12.005"},{"issue":"1","key":"9778_CR12","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0022-3476(72)80470-0","volume":"80","author":"H Yoshioka","year":"1972","unstructured":"Yoshioka H, Monma T, Matsuda S. Placental transfer of gentamicin. J Pediatr. 1972;80(1):121\u20133.","journal-title":"J Pediatr"},{"issue":"7","key":"9778_CR13","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/S0002-9378(16)33336-1","volume":"124","author":"AJ Weinstein","year":"1976","unstructured":"Weinstein AJ, Gibbs RS, Gallagher M. Placental transfer of clindamycin and gentamicin in term pregnancy. Am J Obstet Gynecol. 1976;124(7):688\u201391.","journal-title":"Am J Obstet Gynecol"},{"issue":"2","key":"9778_CR14","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1203\/00006450-197502000-00009","volume":"9","author":"RE Kauffman","year":"1975","unstructured":"Kauffman RE, Morris JA, Azarnoff DL. Placental transfer and fetal urinary excretion of gentamicin during constant rate maternal infusion. Pediatr Res. 1975;9(2):104\u20137.","journal-title":"Pediatr Res"},{"issue":"Pt 2","key":"9778_CR15","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1042\/bj20021958","volume":"373","author":"M Larsson","year":"2003","unstructured":"Larsson M, Hjalm G, Sakwe AM, Engstrom A, Hoglund AS, Larsson E, et al. Selective interaction of megalin with postsynaptic density-95 (PSD-95)-like membrane-associated guanylate kinase (MAGUK) proteins. Biochem J. 2003;373(Pt 2):381\u201391.","journal-title":"Biochem J"},{"issue":"10","key":"9778_CR16","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1681\/ASN.V9101767","volume":"9","author":"R Nielsen","year":"1998","unstructured":"Nielsen R, Birn H, Moestrup SK, Nielsen M, Verroust P, Christensen EI. Characterization of a kidney proximal tubule cell line, LLC-PK1, expressing endocytotic active megalin. J Am Soc Nephrol. 1998;9(10):1767\u201376.","journal-title":"J Am Soc Nephrol"},{"issue":"4","key":"9778_CR17","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1128\/AAC.50.4.1213-1221.2006","volume":"50","author":"H Servais","year":"2006","unstructured":"Servais H, Jossin Y, Van Bambeke F, Tulkens PM, Mingeot-Leclercq MP. Gentamicin causes apoptosis at low concentrations in renal LLC-PK1 cells subjected to electroporation. Antimicrob Agents Chemother. 2006;50(4):1213\u201321.","journal-title":"Antimicrob Agents Chemother"},{"issue":"(2","key":"9778_CR18","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1002\/jcp.20874","volume":"21","author":"Q Hao","year":"2007","unstructured":"Hao Q, Hong SH, Maret W. Lipid raft-dependent endocytosis of metallothionein in HepG2 cells. J Cell Physiol. 2007;21((2):428\u201335.","journal-title":"J Cell Physiol"},{"issue":"5","key":"9778_CR19","doi-asserted-by":"crossref","first-page":"C1105","DOI":"10.1152\/ajpcell.00514.2002","volume":"284","author":"T Takeda","year":"2003","unstructured":"Takeda T, Yamazaki H, Farquhar MG. Identification of an apical sorting determinant in the cytoplasmic tail of megalin. Am J Physiol Cell Physiol. 2003;284(5):C1105\u201313.","journal-title":"Am J Physiol Cell Physiol"},{"issue":"4","key":"9778_CR20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25(4):402\u20138.","journal-title":"Methods"},{"issue":"16","key":"9778_CR21","doi-asserted-by":"crossref","first-page":"8612","DOI":"10.1073\/pnas.93.16.8612","volume":"93","author":"SK Moestrup","year":"1996","unstructured":"Moestrup SK, Birn H, Fischer PB, Petersen CM, Verroust PJ, Sim RB, et al. Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis. Proc Natl Acad Sci U S A. 1996;93(16):8612\u20137.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"9778_CR22","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/S0024-3205(99)00345-8","volume":"65","author":"G Decorti","year":"1999","unstructured":"Decorti G, Malusa N, Furlan G, Candussio L, Klugmann FB. Endocytosis of gentamicin in a proximal tubular renal cell line. Life Sci. 1999;65(11):1115\u201324.","journal-title":"Life Sci"},{"key":"9778_CR23","doi-asserted-by":"crossref","unstructured":"Zhai XY, Nielsen R, Birn H, Drumm K, Mildenberger S, Freudinger R, et al. Cubilin- and megalin-mediated uptake of albumin in cultured proximal tubule cells of opossum kidney. Kidney Int. 2000;58(4):1523\u201333.","DOI":"10.1046\/j.1523-1755.2000.00314.x"},{"key":"9778_CR24","doi-asserted-by":"crossref","unstructured":"Moestrup SK, Cui S, Vorum H, Bregengard C, Bjorn SE, Norris K, et al. Evidence that epithelial glycoprotein 330\/megalin mediates uptake of polybasic drugs. J Clin Invest. 1995;96(3):1404\u201313.","DOI":"10.1172\/JCI118176"},{"issue":"9","key":"9778_CR25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0025065","volume":"6","author":"T Prabakaran","year":"2011","unstructured":"Prabakaran T, Nielsen R, Larsen JV, Sorensen SS, Feldt-Rasmussen U, Saleem MA, et al. Receptor-mediated endocytosis of alpha-galactosidase A in human podocytes in Fabry disease. PLoS One. 2011;6(9), e25065.","journal-title":"PLoS One"},{"issue":"(5","key":"9778_CR26","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1172\/JCI112463","volume":"77","author":"P Tran Ba Huy","year":"1986","unstructured":"Tran Ba Huy P, Bernard P, Schacht J. Kinetics of gentamicin uptake and release in the rat. Comparison of inner ear tissues and fluids with other organs. J Clin Invest. 1986;77((5):1492\u2013500.","journal-title":"J Clin Invest"},{"issue":"6","key":"9778_CR27","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1128\/AAC.11.6.974","volume":"11","author":"ML Kornguth","year":"1977","unstructured":"Kornguth ML, Kunin CM. Distribution of gentamicin and amikacin in rabbit tissues. Antimicrob Agents Chemother. 1977;11(6):974\u20137.","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"9778_CR28","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1159\/000199446","volume":"14","author":"J Tauris","year":"2009","unstructured":"Tauris J, Christensen EI, Nykjaer A, Jacobsen C, Petersen CM, Ovesen T. Cubilin and megalin co-localize in the neonatal inner ear. Audiol Neurootol. 2009;14(4):267\u201378.","journal-title":"Audiol Neurootol"},{"key":"9778_CR29","doi-asserted-by":"crossref","first-page":"C1596","DOI":"10.1152\/ajpcell.1997.273.5.C1596","volume":"42","author":"F Liu","year":"1997","unstructured":"Liu F, Soares MJ, Audus KL, Liu F, Soares MJ, Audus KL. Permeability properties of monolayers of the human trophoblast cell line BeWo. Am J Physiol. 1997;42:C1596\u2013604.","journal-title":"Am J Physiol"},{"key":"9778_CR30","first-page":"225","volume":"122","author":"CJ Bode","year":"2006","unstructured":"Bode CJ, Jin H, Rytting E, Silverstein PS, Young AM, Audus KL. In vitro models for studying trophoblast transcellular transport. Methods Mol Med. 2006;122:225\u201339.","journal-title":"Methods Mol Med"},{"key":"9778_CR31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1053\/plac.2000.0587","volume":"22","author":"DG Hemmings","year":"2001","unstructured":"Hemmings DG, Lowen B, Sherburne R, Sawicki G, Guilbert LJ. Villous trophoblasts cultured on semi-permeable membranes form an effective barrier to the passage of high and low molecular weight particles. Placenta. 2001;22:70\u20139.","journal-title":"Placenta"}],"container-title":["The AAPS Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/s12248-015-9778-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1208\/s12248-015-9778-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/s12248-015-9778-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T12:23:06Z","timestamp":1652012586000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1208\/s12248-015-9778-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,19]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["9778"],"URL":"https:\/\/doi.org\/10.1208\/s12248-015-9778-9","relation":{},"ISSN":["1550-7416"],"issn-type":[{"value":"1550-7416","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,19]]}}}