{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:27:56Z","timestamp":1770503276332,"version":"3.49.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2008,10,16]],"date-time":"2008-10-16T00:00:00Z","timestamp":1224115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AAPS PharmSciTech"],"published-print":{"date-parts":[[2008,12]]},"DOI":"10.1208\/s12249-008-9149-2","type":"journal-article","created":{"date-parts":[[2008,10,15]],"date-time":"2008-10-15T14:23:04Z","timestamp":1224080584000},"source":"Crossref","is-referenced-by-count":6,"title":["Stability of Poly(\u03b5-caprolactone) Microparticles Containing Brucella ovis Antigens as a Vaccine Delivery System Against Brucellosis"],"prefix":"10.1208","volume":"9","author":[{"given":"Maite","family":"Estevan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Gamazo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Mart\u00ednez-Galan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"Irache","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,10,16]]},"reference":[{"key":"9149_CR1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1099\/00222615-43-5-344","volume":"43","author":"R. A. Bowden","year":"1995","unstructured":"R. A. Bowden, A. Cloeckaert, M. S. Zygmunt, and G. Dubray. Outer-membrane protein- and rough lipopolysaccharide-specific monoclonal antibodies protect mice against Brucella ovis. J. Med. Microbiol. 43:344\u2013347 (1995).","journal-title":"J. Med. Microbiol."},{"key":"9149_CR2","first-page":"153","volume-title":"Animal Brucellosis","author":"A. P. MacMillan","year":"1990","unstructured":"A. P. MacMillan. Conventional serological tests. In K. H. Nielsen, and J. R. Duncan (eds.), Animal Brucellosis, CRC, Boca Raton, Florida, 1990, pp. 153\u2013197."},{"key":"9149_CR3","first-page":"793","volume":"371","author":"G. G. Alton","year":"1967","unstructured":"G. G. Alton, and S. S. Elberg. Rev1 Brucella melitensis vaccine: a review of ten years of study. Vet. Bull. 371:793\u2013800 (1967).","journal-title":"Vet. Bull."},{"key":"9149_CR4","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.jconrel.2004.09.030","volume":"102","author":"G. Rosa De","year":"2005","unstructured":"G. De Rosa, D. Larobina, M. I. La Rotonda, P. Musto, F. Quaglia, and F. Ungaro. How cyclodextrin incorporation affects the properties of protein-loaded PLGA-based microspheres: the case of insulin\/hydroxypropyl-\u03b2-cyclodextrin system. J. Contr. Rel. 102:71\u201383 (2005).","journal-title":"J. Contr. Rel."},{"key":"9149_CR5","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1016\/0140-6736(93)91571-3","volume":"342","author":"J. M. Blasco","year":"1993","unstructured":"J. M. Blasco, and R. D\u00edaz. Brucella melitensis Rev1 vaccine as a cause of human brucellosis. Lancet. 342:805 (1993).","journal-title":"Lancet"},{"key":"9149_CR6","first-page":"41","volume-title":"Brucellosis: Clinical and Laboratory Aspects","author":"P. L. Nicoletti","year":"1989","unstructured":"P. L. Nicoletti. Relationship between animal and human disease. In E. J. Young, and M. J. Corbel (eds.), Brucellosis: Clinical and Laboratory Aspects, CRC, Boca Raton, Florida, 1989, pp. 41\u201351."},{"key":"9149_CR7","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/0165-2427(93)90198-D","volume":"37","author":"J. M. Blasco","year":"1993","unstructured":"J. M. Blasco, C. Gamazo, A. J. Winter, M. P. Jim\u00e9nez de Bag\u00fc\u00e9s, C. Mar\u00edn, M. Barber\u00e1n, I. Moriy\u00f3n, B. Alonso-Urmeneta, and R. D\u00edaz. Evaluation of whole cell and subcellular vaccines against Brucella ovis in rams. Vet. Immunol. Immunopathol. 37:257\u2013270 (1993).","journal-title":"Vet. Immunol. Immunopathol."},{"key":"9149_CR8","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1128\/IAI.57.5.1419-1426.1989","volume":"57","author":"C. Gamazo","year":"1989","unstructured":"C. Gamazo, A. J. Winter, I. Moriy\u00f3n, J. L. Riezu-Boj, J. M. Blasco, and R. D\u00edaz. Comparative analysis of proteins extracted by hot saline or release spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis. Infect. Immun. 57:1419\u20131426 (1989).","journal-title":"Infect. Immun."},{"key":"9149_CR9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1128\/IAI.58.2.489-494.1990","volume":"58","author":"J. I. Riezu-Boj","year":"1990","unstructured":"J. I. Riezu-Boj, I. Moriy\u00f3n, J. M. Blasco, C. Gamazo, and R. D\u00edaz. Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. Infect. Immun. 58:489\u2013494 (1990).","journal-title":"Infect. Immun."},{"key":"9149_CR10","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/S1286-4579(00)00317-8","volume":"2","author":"R. A. Bowden","year":"2000","unstructured":"R. A. Bowden, S. M. Estein, M. S. Zygmunt, G. Dubray, and A. Cloeckaert. Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies. Microbes. Infect. 2:481\u2013488 (2000).","journal-title":"Microbes. Infect."},{"key":"9149_CR11","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1128\/IAI.62.2.632-638.1994","volume":"62","author":"M. P. Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1994","unstructured":"M. P. Jim\u00e9nez de Bag\u00fc\u00e9s, P. H. Elzer, M. Barber\u00e1n, J. M. Blasco, C. M. Mar\u00edn, C. Gamazo, and A. J. Winter. Protective immunity to Brucella ovis in BALB\/c mice following recovery from primary infection or immunization with subcellular vaccines. Infect. Immun. 62:632\u2013638 (1994).","journal-title":"Infect. Immun."},{"key":"9149_CR12","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.1128\/IAI.58.12.3980-3987.1990","volume":"58","author":"A. Cloeckaert","year":"1990","unstructured":"A. Cloeckaert, P. de Wergifosse, G. Dubray, and J. N. Limet. Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labelling for electron microscopy and enzyme-linked immunosorbent assay. Infect. Immun. 58:3980\u20133987 (1990).","journal-title":"Infect. Immun."},{"key":"9149_CR13","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S0378-1135(02)00211-0","volume":"90","author":"A. Cloeckaert","year":"2002","unstructured":"A. Cloeckaert, N. Vizca\u00edno, J. -Y. Paquet, R. A. Bowden, and P. H. Elzer. Major outer membrane proteins of Brucella spp.: past, present and future. Vet. Microbiol. 90:229\u2013247 (2002).","journal-title":"Vet. Microbiol."},{"key":"9149_CR14","doi-asserted-by":"publisher","first-page":"4326","DOI":"10.1128\/IAI.71.8.4326-4332.2003","volume":"71","author":"I. Salhi","year":"2003","unstructured":"I. Salhi, R. A. Boigegrain, J. Machold, C. Weise, A. Cloeckaert, and B. Rouot. Characterization of new members of the Group 3 outer membrane protein family of Brucella spp. Infect. Immun. 71:4326\u20134332 (2003).","journal-title":"Infect. Immun."},{"key":"9149_CR15","doi-asserted-by":"crossref","first-page":"4960","DOI":"10.1128\/IAI.67.9.4960-4962.1999","volume":"67","author":"A. Tibor","year":"1999","unstructured":"A. Tibor, B. Decelle, and J. J. Letesson. Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins. Infect. Immun. 67:4960\u20134962 (1999).","journal-title":"Infect. Immun."},{"key":"9149_CR16","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1177\/104063879500700208","volume":"7","author":"R. Kittelberger","year":"1995","unstructured":"R. Kittelberger, F. Hilbink, M. F. Hansen, G. P. Ross, G. W. de Lisle, A. Cloeckaert, and J. de Bruyn. Identification and characterization of immunodominant antigens during the course of infection with Brucella ovis. J. Vet. Diagn. Invest. 7:210\u2013218 (1995).","journal-title":"J. Vet. Diagn. Invest."},{"key":"9149_CR17","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1080\/10611860290022642","volume":"10","author":"M. Murillo","year":"2002","unstructured":"M. Murillo, C. Gamazo, J. M. Irache, and M. M. Go\u00f1i. Polyester microparticles as a vaccine delivery system for brucellosis: influence of the polymer on release, phagocytosis and toxicity. J. Drug Target. 10:211\u2013219 (2002).","journal-title":"J. Drug Target."},{"key":"9149_CR18","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1016\/S0264-410X(01)00177-3","volume":"19","author":"M. Murillo","year":"2001","unstructured":"M. Murillo, M. J. Grill\u00f3, J. Re\u00f1\u00e9, C. M. Mar\u00edn, M. Barber\u00e1n, M. M. Go\u00f1i, J. M. Blasco, J. M. Irache, and C. Gamazo. A Brucella ovis antigenic complex bearing poly-epsilon-caprolactone microparticles confer protection against experimental brucellosis in mice. Vaccine. 19:4099\u20134106 (2001).","journal-title":"Vaccine"},{"key":"9149_CR19","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/S0168-3659(02)00371-1","volume":"86","author":"G. Garc\u00eda del Barrio","year":"2003","unstructured":"G. Garc\u00eda del Barrio, F. J. Novo, and J. M. Irache. Loading of plasmid DNA into PLGA microparticles using TROMS (Total Recirculation One-Machine System): evaluation of its integrity and controlled release properties. J. Control. Rel. 86:123\u2013130 (2003).","journal-title":"J. Control. Rel."},{"key":"9149_CR20","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1016\/j.vaccine.2005.10.042","volume":"24","author":"M. P. Mu\u00f1oz","year":"2006","unstructured":"M. P. Mu\u00f1oz, M. Estevan, C. M. Mar\u00edn, M. J. De Miguel, M. J. Grill\u00f3, M. Barber\u00e1n, J. M. Irache, J. M. Blasco, and C. Gamazo. Brucella outer membrane complex-loaded microparticles as a vaccine against Brucella ovis in rams. Vaccine. 24:1897\u20131905 (2006).","journal-title":"Vaccine"},{"key":"9149_CR21","doi-asserted-by":"publisher","first-page":"4179","DOI":"10.1016\/j.vaccine.2006.01.038","volume":"24","author":"M. Estevan","year":"2006","unstructured":"M. Estevan, C. Gamazo, M. J. Grill\u00f3, G. Garc\u00eda del Barrio, J. M. Blasco, and J. M. Irache. Experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against Brucella melitensis in mice. Vaccine. 24:4179\u20134187 (2006).","journal-title":"Vaccine"},{"key":"9149_CR22","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1080\/02652040500435253","volume":"23","author":"M. Estevan","year":"2006","unstructured":"M. Estevan, C. Gamazo, G. Gonz\u00e1lez-Gaitano, and J. M. Irache. Optimization of the entrapment of bacterial cell envelope extracts into microparticles for vaccine delivery. J. Microencapsul. 23:169\u2013181 (2006).","journal-title":"J. Microencapsul."},{"key":"9149_CR23","unstructured":"Instructions BCA Protein Assay Reagent Kit. Pierce Biotechnology, Rockford, USA, 2002."},{"key":"9149_CR24","doi-asserted-by":"publisher","first-page":"197","DOI":"10.3109\/02652049709015333","volume":"14","author":"J. P. McGee","year":"1997","unstructured":"J. P. McGee, M. Singh, X. M. Li, H. Qiu, and D. T. O\u2019Hagan. The encapsulation of a model protein in poly(d,l lactide-co-glycolide) microparticles of various sizes: an evaluation of process reproducibility. J. Microencapsul. 14:197\u2013210 (1997).","journal-title":"J. Microencapsul."},{"key":"9149_CR25","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/0168-3659(95)00052-A","volume":"36","author":"D. T. O\u2019Hagan","year":"1995","unstructured":"D. T. O\u2019Hagan, J. P. McGee, R. Boyle, D. Gumaer, X. M. Li, B. Potts, C. Y. Wang, and W. C. Koff. The preparation, characterization and pre-clinical evaluation of an orally administered HIV-1 vaccine, consisting of a branched peptide immunogen entrapped in controlled release microparticles. J. Control. Rel. 36:75\u201384 (1995).","journal-title":"J. Control. Rel."},{"key":"9149_CR26","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1002\/mabi.200600070","volume":"6","author":"H. Abe","year":"2006","unstructured":"H. Abe. Thermal degradation of environmentally degradable poly(hydroxyalkanoic acid)s. Macromol. Biosci. 6:469\u2013486 (2006).","journal-title":"Macromol. Biosci."},{"key":"9149_CR27","first-page":"71","volume-title":"Biodegradable Polymers as Drug Delivery Systems","author":"C. G. Pitt","year":"1990","unstructured":"C. G. Pitt. Poly(\u03b5-caprolactone) and its copolymers. In M. Chasin, and R. Langer (eds.), Biodegradable Polymers as Drug Delivery Systems, Marcel Dekker, New York, 1990, pp. 71\u2013120."},{"key":"9149_CR28","first-page":"219","volume":"98","author":"M. Yang","year":"2004","unstructured":"M. Yang, F. Cui, B. You, L. Wang, L. Zhang, and Y. Kawashima. A novel pH-dependent gradient-release delivery system for nitrendipine I. Manufacturing, evaluation in vitro and bioavailability in healthy dogs. J. Control. Rel. 98:219\u2013229 (2004).","journal-title":"J. Control. Rel."},{"key":"9149_CR29","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/0168-3659(94)90233-X","volume":"32","author":"C. Yan","year":"1994","unstructured":"C. Yan, J. H. Resau, J. Heweston, M. West, W. L. Rill, and M. Kende. Characterisation and morphological analysis of protein loaded poly(lactide-co-glycolide) microparticles prepared by water-in-oil-in-water emulsion technique. J. Control. Rel. 32:231\u2013241 (1994).","journal-title":"J. Control. Rel."},{"key":"9149_CR30","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/S0378-5173(99)00109-X","volume":"184","author":"M. A. Benoit","year":"1999","unstructured":"M. A. Benoit, B. Baras, and J. Gillard. Preparation and characterization of protein-loaded poly(\u03b5-caprolactone) microparticles for oral vaccine delivery. Int. J. Pharm. 184:73\u201384 (1999).","journal-title":"Int. J. Pharm."},{"key":"9149_CR31","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1002\/(SICI)1097-4636(199707)36:1<125::AID-JBM15>3.0.CO;2-H","volume":"36","author":"S. K. Mallapragada","year":"1997","unstructured":"S. K. Mallapragada, N. A. Peppas, and P. Colombo. Crystal dissolution-controlled release systems. II. Metronidazole release from semicrystalline poly(vinyl alcohol) systems. J. Biomed. Mat. Res. 36:125\u2013130 (1997).","journal-title":"J. Biomed. Mat. Res."},{"key":"9149_CR32","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/S0168-3659(03)00367-5","volume":"92","author":"J. C. Jeong","year":"2003","unstructured":"J. C. Jeong, J. Lee, and K. Cho. Effects of crystalline microstructure on drug release behaviour of poly(\u03b5-caprolactone) microspheres. J. Control. Rel. 92:249\u2013258 (2003).","journal-title":"J. Control. Rel."},{"key":"9149_CR33","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1080\/02652040210162540","volume":"19","author":"W. J. Lin","year":"2002","unstructured":"W. J. Lin, and C. C. Yu. The effect of solvent removal conditions on performance and release property of protein-loaded microparticles. J. Microencapsul. 19:767\u2013773 (2002).","journal-title":"J. Microencapsul."},{"key":"9149_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijpharm.2004.01.044","volume":"278","author":"V. R. Sinha","year":"2004","unstructured":"V. R. Sinha, K. Bansal, R. Kaushik, R. Kumria, and A. Trehan. Poly-\u025b-caprolactone microspheres and nanospheres: an overview. Int. J. Pharm. 278:1\u201323 (2004).","journal-title":"Int. J. Pharm."}],"container-title":["AAPS PharmSciTech"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/s12249-008-9149-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1208\/s12249-008-9149-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/s12249-008-9149-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1208\/s12249-008-9149-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T08:37:18Z","timestamp":1589013438000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1208\/s12249-008-9149-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,16]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["9149"],"URL":"https:\/\/doi.org\/10.1208\/s12249-008-9149-2","relation":{},"ISSN":["1530-9932"],"issn-type":[{"value":"1530-9932","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,16]]},"article-number":"1063"}}