{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:05:35Z","timestamp":1789142735688,"version":"build-2803163510"},"reference-count":40,"publisher":"The Endocrine Society","issue":"10","license":[{"start":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T00:00:00Z","timestamp":1592438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800630"],"award-info":[{"award-number":["81800630"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["19ZR1440800"],"award-info":[{"award-number":["19ZR1440800"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nature Science Key Program of College and University of Anhui Province","award":["KJ2017A236"],"award-info":[{"award-number":["KJ2017A236"]}]},{"name":"Key scientificv and technological project of Songjiang District","award":["18sjkjgg40"],"award-info":[{"award-number":["18sjkjgg40"]}]},{"name":"Hongkou District Health Committee Hongkou hygiene","award":["1702-02"],"award-info":[{"award-number":["1702-02"]}]},{"name":"Hongkou District Health Committee","award":["HGY-KY-2018-37"],"award-info":[{"award-number":["HGY-KY-2018-37"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Context<\/jats:title>\n                  <jats:p>Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. Chaperone-mediated autophagy (CMA), 1 type of autophagy, is thought to promote or suppress cancer development in different cancer types. However, the effect of CMA on PTC development and the underlying mechanisms remain unknown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To determine whether CMA plays implied critical roles in the development of PTC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>We investigated the association between CMA and PTC development in PTC tissues and normal thyroid tissues by detecting the key protein of CMA, lysosome-associated membrane protein type 2A (LAMP2A), using quantitative polymerase chain reaction (PCR) and immunohistochemistry, which were further validated in the TGCA dataset. The effect of CMA on PTC development was studied by cell proliferation, migration, and apoptosis assays. The underlying mechanisms of peroxisome proliferator-activated receptor \u03b3 (PPAR\u03b3)-stromal cell-derived factor 1 (SDF1)\/ C-X-C motif chemokine receptor 4 (CXCR4) signaling were clarified by western blotting, quantitative PCR, and rescue experiments. Knockdown and tamoxifen were used to analyze the effect of estrogen receptor (ER) \u03b1 on CMA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our study confirmed that CMA, indicated by LAMP2A expression, was significantly increased in PTC tumor tissues and cell lines, and was associated with tumor size and lymph node metastasis of patients. Higher CMA in PTC promoted tumor cell proliferation and migration, thereby promoting tumor growth and metastasis. These effects of CMA on PTC were exerted by decreasing PPAR\u03b3 protein expression to enhance SDF1 and CXCR4 expression. Furthermore, CMA was found positively regulated by ER\u03b1 signaling in PTC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our investigation identified CMA regulated by ER\u03b1 promoting PTC tumor progression that enhanced tumor cell proliferation and migration by targeting PPAR\u03b3-SDF1\/CXCR4 signaling, representing a potential target for treatment of PTC.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/clinem\/dgaa366","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T22:33:29Z","timestamp":1592433209000},"page":"3308-3323","source":"Crossref","is-referenced-by-count":14,"title":["Chaperone-mediated Autophagy Governs Progression of Papillary Thyroid Carcinoma via PPAR\u03b3-SDF1\/CXCR4 Signaling"],"prefix":"10.1210","volume":"105","author":[{"given":"Hong","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xie","sequence":"additional","affiliation":[{"name":"Shanghai TCM-Integrated hospital (endocrinology department), Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaogen","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijie","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongde","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4294-0274","authenticated-orcid":false,"given":"Huanbai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"issue":"2","key":"2021122105421424600_CIT0001","doi-asserted-by":"crossref","first-page":"E276","DOI":"10.1210\/jc.2013-2503","article-title":"The increase in thyroid cancer incidence during the last four decades is accompanied by a high frequency of BRAF mutations and a sharp increase in RAS mutations","volume":"99","author":"Jung","year":"2014","journal-title":"J Clin Endocrinol Metab."},{"issue":"2","key":"2021122105421424600_CIT0002","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4143\/crt.2015.118","article-title":"BRAF-activated long noncoding RNA modulates papillary thyroid carcinoma cell proliferation through regulating thyroid stimulating hormone receptor","volume":"48","author":"Zheng","year":"2016","journal-title":"Cancer Res Treat."},{"issue":"3","key":"2021122105421424600_CIT0003","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.cell.2014.09.050","article-title":"Integrated genomic characterization of papillary thyroid carcinoma","volume":"159","author":"Cancer Genome Atlas Research Network","year":"2014","journal-title":"Cell."},{"issue":"6","key":"2021122105421424600_CIT0004","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1053\/j.seminoncol.2010.10.008","article-title":"Differentiated thyroid cancer","volume":"37","author":"Burns","year":"2010","journal-title":"Semin Oncol."},{"issue":"5-6","key":"2021122105421424600_CIT0005","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1615\/CritRevOncog.2018027913","article-title":"Autophagy and hallmarks of cancer","volume":"23","author":"Huang","year":"2018","journal-title":"Crit Rev Oncog."},{"issue":"9","key":"2021122105421424600_CIT0006","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1038\/s41388-017-0046-6","article-title":"Dual role of autophagy in hallmarks of cancer","volume":"37","author":"Singh","year":"2018","journal-title":"Oncogene."},{"key":"2021122105421424600_CIT0007","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.nbd.2018.05.010","article-title":"Chaperone-mediated autophagy: advances from bench to bedside","volume":"122","author":"Li","year":"2019","journal-title":"Neurobiol Dis."},{"issue":"6","key":"2021122105421424600_CIT0008","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/s41580-018-0001-6","article-title":"The coming of age of chaperone-mediated autophagy","volume":"19","author":"Kaushik","year":"2018","journal-title":"Nat Rev Mol Cell Biol."},{"issue":"1","key":"2021122105421424600_CIT0009","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/cr.2013.153","article-title":"Chaperone-mediated autophagy: roles in disease and aging","volume":"24","author":"Cuervo","year":"2014","journal-title":"Cell Res."},{"issue":"109","key":"2021122105421424600_CIT0010","doi-asserted-by":"crossref","first-page":"109ra117","DOI":"10.1126\/scitranslmed.3003182","article-title":"Chaperone-mediated autophagy is required for tumor growth","volume":"3","author":"Kon","year":"2011","journal-title":"Sci Transl Med."},{"issue":"1","key":"2021122105421424600_CIT0011","doi-asserted-by":"crossref","first-page":"4759","DOI":"10.1038\/s41598-017-04994-x","article-title":"Downregulation of ATG5-dependent macroautophagy by chaperone-mediated autophagy promotes breast cancer cell metastasis","volume":"7","author":"Han","year":"2017","journal-title":"Sci Rep."},{"issue":"3","key":"2021122105421424600_CIT0012","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1080\/15548627.2015.1136770","article-title":"Chaperone-mediated autophagy regulates proliferation by targeting RND3 in gastric cancer","volume":"12","author":"Zhou","year":"2016","journal-title":"Autophagy."},{"issue":"15","key":"2021122105421424600_CIT0013","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1101\/gad.220897.113","article-title":"Chaperone-mediated autophagy degrades mutant p53","volume":"27","author":"Vakifahmetoglu-Norberg","year":"2013","journal-title":"Genes Dev."},{"issue":"4","key":"2021122105421424600_CIT0014","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/j.bbrc.2016.12.037","article-title":"Chaperone-mediated autophagy promotes lung cancer cell survival through selective stabilization of the pro-survival protein, MCL1","volume":"482","author":"Suzuki","year":"2017","journal-title":"Biochem Biophys Res Commun."},{"issue":"11","key":"2021122105421424600_CIT0015","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.4161\/auto.21654","article-title":"LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy","volume":"8","author":"Saha","year":"2012","journal-title":"Autophagy."},{"issue":"5","key":"2021122105421424600_CIT0016","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1083\/jcb.201503044","article-title":"Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death","volume":"210","author":"Xia","year":"2015","journal-title":"J Cell Biol."},{"key":"2021122105421424600_CIT0017","doi-asserted-by":"crossref","first-page":"712561","DOI":"10.1155\/2013\/712561","article-title":"Stimulation of TLR4 by LMW-HA induces metastasis in human papillary thyroid carcinoma through CXCR7","volume":"2013","author":"Dang","year":"2013","journal-title":"Clin Dev Immunol."},{"issue":"2","key":"2021122105421424600_CIT0018","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/(SICI)1096-9098(199610)63:2<112::AID-JSO8>3.0.CO;2-H","article-title":"Establishment of xenografts and cell lines from well-differentiated human thyroid carcinoma","volume":"63","author":"Lin","year":"1996","journal-title":"J Surg Oncol."},{"issue":"12","key":"2021122105421424600_CIT0019","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1111\/j.1349-7006.1989.tb01645.x","article-title":"Presence of aberrant transcripts of ret proto-oncogene in a human papillary thyroid carcinoma cell line","volume":"80","author":"Ishizaka","year":"1989","journal-title":"Jpn J Cancer Res."},{"issue":"8","key":"2021122105421424600_CIT0020","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1089\/thy.2007.0097","article-title":"Molecular and genotypic characterization of human thyroid follicular cell carcinoma-derived cell lines","volume":"17","author":"Meireles","year":"2007","journal-title":"Thyroid."},{"issue":"2","key":"2021122105421424600_CIT0021","doi-asserted-by":"crossref","first-page":"E204","DOI":"10.1210\/jc.2014-2883","article-title":"miR-219-5p modulates cell growth of papillary thyroid carcinoma by targeting estrogen receptor \u03b1","volume":"100","author":"Huang","year":"2015","journal-title":"J Clin Endocrinol Metab."},{"issue":"1","key":"2021122105421424600_CIT0022","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1186\/s12885-019-6306-9","article-title":"EZH2 upregulation by ER\u03b1 induces proliferation and migration of papillary thyroid carcinoma","volume":"19","author":"Xue","year":"2019","journal-title":"BMC Cancer."},{"issue":"7","key":"2021122105421424600_CIT0023","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.4161\/auto.28771","article-title":"Autophagy regulates the therapeutic potential of mesenchymal stem cells in experimental autoimmune encephalomyelitis","volume":"10","author":"Dang","year":"2014","journal-title":"Autophagy."},{"issue":"9","key":"2021122105421424600_CIT0024","doi-asserted-by":"crossref","first-page":"E1388","DOI":"10.1210\/jc.2011-0345","article-title":"MiR-1 is a tumor suppressor in thyroid carcinogenesis targeting CCND2, CXCR4, and SDF-1alpha","volume":"96","author":"Leone","year":"2011","journal-title":"J Clin Endocrinol Metab."},{"issue":"2","key":"2021122105421424600_CIT0025","doi-asserted-by":"crossref","first-page":"695","DOI":"10.3892\/mmr.2014.2232","article-title":"Peroxisome proliferator\u2011activated receptor \u03b3 agonist rosiglitazone inhibits migration and invasion of prostate cancer cells through inhibition of the CXCR4\/CXCL12 axis","volume":"10","author":"Qin","year":"2014","journal-title":"Mol Med Rep."},{"key":"2021122105421424600_CIT0026","doi-asserted-by":"crossref","first-page":"769413","DOI":"10.1155\/2008\/769413","article-title":"CXCR4 in Cancer and Its Regulation by PPARgamma","volume":"2008","author":"Richard","year":"2008","journal-title":"PPAR Res."},{"issue":"2","key":"2021122105421424600_CIT0027","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1038\/modpathol.2010.129","article-title":"Papillary thyroid carcinoma: an update","volume":"24","author":"LiVolsi","year":"2011","journal-title":"Mod Pathol."},{"issue":"1","key":"2021122105421424600_CIT0028","first-page":"308","article-title":"Tamoxifen inhibits growth, migration, and invasion of human follicular and papillary thyroid cancer cells in vitro and in vivo","volume":"80","author":"Hoelting","year":"1995","journal-title":"J Clin Endocrinol Metab."},{"issue":"3","key":"2021122105421424600_CIT0029","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1002\/jcp.20551","article-title":"Steering estrogen signals from the plasma membrane to the nucleus: two sides of the coin","volume":"207","author":"Manavathi","year":"2006","journal-title":"J Cell Physiol."},{"key":"2021122105421424600_CIT0030","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.med.53.082901.104018","article-title":"The mechanisms of action of PPARs","volume":"53","author":"Berger","year":"2002","journal-title":"Annu Rev Med."},{"issue":"12","key":"2021122105421424600_CIT0031","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.2174\/1389450120666190527115538","article-title":"PPAR\u03b3: potential therapeutic target for ailments beyond diabetes and its natural agonism","volume":"20","author":"Shafi","year":"2019","journal-title":"Curr Drug Targets."},{"issue":"10","key":"2021122105421424600_CIT0032","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/nrendo.2014.115","article-title":"Pax-8-PPAR-\u03b3 fusion protein in thyroid carcinoma","volume":"10","author":"Raman","year":"2014","journal-title":"Nat Rev Endocrinol."},{"key":"2021122105421424600_CIT0033","doi-asserted-by":"crossref","first-page":"6200","DOI":"10.12659\/MSM.910036","article-title":"Rosiglitazone reduces apoptosis and inflammation in lipopolysaccharide-induced human umbilical vein endothelial cells","volume":"24","author":"Ji","year":"2018","journal-title":"Med Sci Monit."},{"issue":"5","key":"2021122105421424600_CIT0034","first-page":"1215","article-title":"Thiazolidinedione drugs down-regulate CXCR4 expression on human colorectal cancer cells in a peroxisome proliferator activated receptor gamma-dependent manner","volume":"30","author":"Richard","year":"2007","journal-title":"Int J Oncol."},{"issue":"40","key":"2021122105421424600_CIT0035","doi-asserted-by":"crossref","first-page":"65109","DOI":"10.18632\/oncotarget.11371","article-title":"Ligand-activated PPAR\u03b3 downregulates CXCR4 gene expression through a novel identified PPAR response element and inhibits breast cancer progression","volume":"7","author":"Rovito","year":"2016","journal-title":"Oncotarget."},{"issue":"1","key":"2021122105421424600_CIT0036","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/cncr.28383","article-title":"The cross-talk between estrogen receptor and peroxisome proliferator-activated receptor gamma in thyroid cancer","volume":"120","author":"Chu","year":"2014","journal-title":"Cancer."},{"issue":"2","key":"2021122105421424600_CIT0037","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1677\/JOE-06-0193","article-title":"Expression and role of estrogen receptor alpha and beta in medullary thyroid carcinoma: different roles in cancer growth and apoptosis","volume":"195","author":"Cho","year":"2007","journal-title":"J Endocrinol."},{"key":"2021122105421424600_CIT0038","doi-asserted-by":"crossref","first-page":"105380","DOI":"10.1016\/j.jsbmb.2019.105380","article-title":"Role of estrogen and its receptors mediated-autophagy in cell fate and human diseases","volume":"191","author":"Wei","year":"2019","journal-title":"J Steroid Biochem Mol Biol."},{"issue":"11","key":"2021122105421424600_CIT0039","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1038\/ni.3003","article-title":"Chaperone-mediated autophagy regulates T cell responses through targeted degradation of negative regulators of T cell activation","volume":"15","author":"Valdor","year":"2014","journal-title":"Nat Immunol."},{"issue":"1","key":"2021122105421424600_CIT0040","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1038\/s41467-017-01609-x","article-title":"Phosphorylation of LAMP2A by p38 MAPK couples ER stress to chaperone-mediated autophagy","volume":"8","author":"Li","year":"2017","journal-title":"Nat Commun."}],"container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/advance-article-pdf\/doi\/10.1210\/clinem\/dgaa366\/33565261\/dgaa366.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/105\/10\/3308\/41828564\/dgaa366.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/105\/10\/3308\/41828564\/dgaa366.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T05:43:20Z","timestamp":1640065400000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/105\/10\/3308\/5859150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,18]]},"references-count":40,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,6,18]]},"published-print":{"date-parts":[[2020,10,1]]}},"URL":"https:\/\/doi.org\/10.1210\/clinem\/dgaa366","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,10]]},"published":{"date-parts":[[2020,6,18]]}}}