{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:19:38Z","timestamp":1772997578760,"version":"3.50.1"},"reference-count":44,"publisher":"The Endocrine Society","issue":"8","license":[{"start":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T00:00:00Z","timestamp":1591747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"ECTS Clinical Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Context<\/jats:title>\n                  <jats:p>Serum expression of microRNAs (miRs) related to bone metabolism is affected by antiosteoporotic treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To investigate the effect of sequential treatments on miR expression in postmenopausal women with osteoporosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>Observational, open label, nonrandomized clinical trial.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Setting<\/jats:title>\n                  <jats:p>A single-center outpatient clinic.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Patients and Interventions<\/jats:title>\n                  <jats:p>Denosumab (Dmab) was administered for 12 months in 37 women who were treatment-na\u00efve (na\u00efve group) (n\u2005=\u200511) or previously treated with teriparatide (TPTD group) (n\u2005=\u200520) or zoledronate (ZOL group) (n\u2005=\u20056).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Main Outcome Measures<\/jats:title>\n                  <jats:p>Relative serum expression of miRs linked to bone metabolism at 3 and 6 months of Dmab treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Baseline relative expression of miR-21a-5p, miR-23a-3p, miR-29a-3p, and miR-338-3p was higher in the TPTD group, while the relative expression of miR-21a-5p was lower in the ZOL group compared to the na\u00efve group. Dmab decreased the relative expression of miR-21a-5p at 3 months (fold change [FC] 0.43, P\u2005&amp;lt;\u20050.001) and 6 months (FC 0.34, P\u2005&amp;lt;\u20050.001), and miR-338-3p and miR-2861 at 6 months (FC 0.31, P\u2005=\u20050.041; FC 0.52, P\u2005=\u20050.016, respectively) in the whole cohort. In subgroup analyses, Dmab decreased the relative expression of miR-21a-5p, miR-29a-3p, miR-338-3p, and miR-2861 at 3 months (FC 0.13, P\u2005&amp;lt;\u20050.001; FC 0.68, P\u2005=\u20050.044; FC 0.46, P\u2005=\u20050.012; and FC 0.16, P\u2005&amp;lt;\u20050.001, respectively) and 6 months (FC 0.1, P\u2005&amp;lt;\u20050.001; FC 0.52, P\u2005&amp;lt;\u20050.001; FC 0.04, P\u2005=\u20050.006; and FC 0.2, P\u2005&amp;lt;\u20050.001, respectively) only within the TPTD group.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>TPTD treatment potentially affects the expression of the pro-osteoclastogenic miR-21a-5p and miRs related to the expression of osteoblastic genes RUNX2 (miR23a-3p), COL1 (miR-29a-3p), and HDAC5 (miR-2861), while sequential treatment with Dmab acts in the opposite direction.<\/jats:p>\n               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