{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:16:31Z","timestamp":1792113391808,"version":"4.4.0"},"reference-count":35,"publisher":"The Endocrine Society","issue":"11","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1EY029795"],"award-info":[{"award-number":["RO1EY029795"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760734"],"award-info":[{"award-number":["81760734"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770384"],"award-info":[{"award-number":["81770384"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660313"],"award-info":[{"award-number":["31660313"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["2017FA048"],"award-info":[{"award-number":["2017FA048"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Endocrine Clinical Medical Center of Yunnan Province","award":["ZX2019-02-02"],"award-info":[{"award-number":["ZX2019-02-02"]}]},{"name":"Deparment of Veterans Affairs Merit system","award":["BX003391"],"award-info":[{"award-number":["BX003391"]}]},{"name":"Deparment of Veterans Affairs Merit system","award":["NIH RO1EY029795"],"award-info":[{"award-number":["NIH RO1EY029795"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/clinem\/dgaa538","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T15:56:54Z","timestamp":1597420614000},"page":"3404-3415","source":"Crossref","is-referenced-by-count":94,"title":["MicroRNA-15b Targets VEGF and Inhibits Angiogenesis in Proliferative Diabetic Retinopathy"],"prefix":"10.1210","volume":"105","author":[{"given":"Ying","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Ninth People\u2019s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongli","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taicheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanling","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Second People\u2019s Hospital of Yunnan Province, Kunming, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Yl\u00e4-Herttuala","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio; and Heart Center and Gene Therapy Unit, Kuopio University Hospital, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam S","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Ocular Immunology and Angiogenesis Lab, University of Missouri, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Whaley-Connell","family":"Adam","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, University of Missouri, Columbia, Missouri"},{"name":"Research Service, Harry S. 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