{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T04:08:43Z","timestamp":1768277323305,"version":"3.49.0"},"reference-count":36,"publisher":"The Endocrine Society","issue":"4","license":[{"start":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T00:00:00Z","timestamp":1609372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1025258"],"award-info":[{"award-number":["APP1025258"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Context<\/jats:title><jats:p>Reduced Na+-K+ pump activity is widely reported in preeclampsia and may be caused by a reversible oxidative modification that is a novel pathological feature of preeclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This work aims to determine whether \u03b2\u200a1 subunit (GSS-\u03b2\u200a1) protein glutathionylation of the Na+-K + pump occurs in preeclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The GSS-\u03b21 of the Na+-K+ pump and its subunit expression in human placentas were compared between women with healthy pregnancies and women with preeclampsia.<\/jats:p><jats:p>Human placental samples of pregnant women with preeclampsia (n = 11, mean gestational age 36.5 weeks) were used to examine the GSS-\u03b2\u200a1 of the Na+-K+ pump, compared to healthy pregnancies (n = 11, mean gestational age 39 weeks).<\/jats:p><jats:p>The potential pathogenetic role of GSS-\u03b2\u200a1-mediated Na+-K+ pump dysfunction in preeclampsia was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Protein expression of the \u03b2\u200a1 subunit was unchanged in placentas from women with preeclampsia vs those with normotensive pregnancies. Preeclamptic placentas had a significantly increased GSS-\u03b2\u200a1 of the Na+-K+ pump compared to those from healthy pregnancies, and this was linked to a decrease in \u03b1\u200a1\/\u03b2\u200a1 subunit coimmunoprecipitation. The cytosolic p47phox nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunit and its coimmunoprecipitation with the \u03b1\u200a1 Na+-K+ pump subunit was increased in preeclamptic placentas, thus implicating NADPH oxidase\u2013dependent pump inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The high level of \u03b2\u200a1 pump subunit glutathionylation provides new insights into the mechanism of Na+-K+ pump dysfunction in preeclampsia.<\/jats:p><\/jats:sec>","DOI":"10.1210\/clinem\/dgaa918","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T17:59:51Z","timestamp":1609437591000},"page":"e1091-e1100","source":"Crossref","is-referenced-by-count":4,"title":["S-glutathionylation of the Na+-K+ Pump: A Novel Redox Mechanism in Preeclampsia"],"prefix":"10.1210","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0801-5234","authenticated-orcid":false,"given":"Chia-Chi","family":"Liu","sequence":"first","affiliation":[{"name":"Vascular Immunology Research Laboratory, The Heart Research Institute, University of Sydney, Newtown, NSW, Australia"},{"name":"North Shore Heart Research Group, Kolling Medical Research Institute, University of Sydney, St Leonards, NSW, Australia"},{"name":"School of Medicine, Western Sydney University, Campbelltown, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YunJia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Research Laboratory, The Heart Research Institute, Newtown, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Makris","sequence":"additional","affiliation":[{"name":"Vascular Immunology Research Laboratory, The Heart Research Institute, University of Sydney, Newtown, NSW, Australia"},{"name":"School of Medicine, Western Sydney University, Campbelltown, NSW, Australia"},{"name":"Renal Unit, Liverpool Hospital, Liverpool, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helge H","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"North Shore Heart Research Group, Kolling Medical Research Institute, University of Sydney, St Leonards, NSW, Australia"},{"name":"Department of Cardiology, Royal North Shore Hospital, St Leonards, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemarie","family":"Hennessy","sequence":"additional","affiliation":[{"name":"Vascular Immunology Research Laboratory, The Heart Research Institute, University of Sydney, Newtown, NSW, Australia"},{"name":"School of Medicine, Western Sydney University, Campbelltown, NSW, Australia"},{"name":"Campbelltown Hospital, South Western Sydney Local Health District, Campbelltown, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","published-online":{"date-parts":[[2020,12,31]]},"reference":[{"issue":"1","key":"2021122205213511900_CIT0001","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.preghy.2012.11.001","article-title":"The definition of severe and early-onset preeclampsia. 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