{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:47:44Z","timestamp":1775058464658,"version":"3.50.1"},"reference-count":32,"publisher":"The Endocrine Society","issue":"9","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Endocrine Society of India","award":["ESI\/2019"],"award-info":[{"award-number":["ESI\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Clinicians have long been struggling to find an effective tool to predict onset of puberty.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To explore stimulability of inhibin B after exogenous FSH and its potential role for prediction of onset of puberty.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design and participants<\/jats:title>\n                  <jats:p>Study subjects were enrolled into \u201cexploratory cohort\u201d (n\u2005=\u200542) and \u201cvalidation cohort\u201d (n\u2005=\u200519). The exploratory cohort was further divided into group 1 (healthy children with spontaneous puberty [SP], n\u2005=\u200526) and group 2 (patients with hypogonadotropic hypogonadism [HH], n\u2005=\u200516). The validation cohort included children who presented with complaints of delayed puberty.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Intervention and outcome<\/jats:title>\n                  <jats:p>Participants were subjected to FSH stimulation test and GnRH analogue stimulation test. Cutoffs derived from the exploratory cohort for basal and FSH stimulated inhibin B (FSH-iB) were applied on the validation cohort. Basal LH, GnRH analogue-stimulated LH, basal inhibin B, and FSH-iB were compared with clinical outcomes on a prospective follow-up for prediction of onset of puberty.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>There was statistically significant increment in inhibin B after exogenous FSH in group 1 (SP) in both male (188.8 pg\/mL; P = 0.002) and female (1065 pg\/mL; P = 0.023) subjects. The increment was not statistically significant in group 2 (HH) in both sexes. FSH-iB at a cutoff of 116.14 pg\/mL in males and 116.50 pg\/mL in females had 100% sensitivity and specificity for labelling entry into puberty. On application of these cutoffs on the validation cohort, FSH-iB had 100% positive predictive value, negative predictive value, and diagnostic accuracy for prediction of pubertal onset.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Inhibin B was stimulable in both male and female subjects. FSH-iB can be considered a novel and promising investigation for prediction of onset of puberty. Future studies are required for further validation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/clinem\/dgab357","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T18:03:08Z","timestamp":1621447388000},"page":"e3495-e3505","source":"Crossref","is-referenced-by-count":27,"title":["FSH-stimulated Inhibin B (FSH-iB): A Novel Marker for the Accurate Prediction of Pubertal Outcome in Delayed Puberty"],"prefix":"10.1210","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0641-5701","authenticated-orcid":false,"given":"Shakun","family":"Chaudhary","sequence":"first","affiliation":[{"name":"Department of Endocrinology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6633-923X","authenticated-orcid":false,"given":"Rama","family":"Walia","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-4337","authenticated-orcid":false,"given":"Anil","family":"Bhansali","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3279-6002","authenticated-orcid":false,"given":"Devi","family":"Dayal","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6289-4749","authenticated-orcid":false,"given":"Naresh","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2270-1639","authenticated-orcid":false,"given":"Tulika","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Radiology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0410-8778","authenticated-orcid":false,"given":"Sanjay Kumar","family":"Bhadada","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, PGIMER Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"issue":"1","key":"2021120108562840900_CIT0001","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0197-0070(80)80005-2","article-title":"Normal ages of pubertal events among American males and females","volume":"1","author":"Lee","year":"1980","journal-title":"J Adolesc Health Care."},{"issue":"4","key":"2021120108562840900_CIT0002","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1210\/jcem.87.4.8395","article-title":"Delayed puberty: analysis of a large case series from an academic center","volume":"87","author":"Sedlmeyer","year":"2002","journal-title":"J Clin Endocrinol Metab."},{"issue":"9","key":"2021120108562840900_CIT0003","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nrendo.2015.112","article-title":"Expert consensus document: European Consensus Statement on congenital hypogonadotropic hypogonadism\u2013pathogenesis, diagnosis and treatment","volume":"11","author":"Boehm","year":"2015","journal-title":"Nat Rev Endocrinol."},{"issue":"11","key":"2021120108562840900_CIT0004","doi-asserted-by":"crossref","first-page":"5541","DOI":"10.1210\/jcem.86.11.8031","article-title":"Importance of inhibin B in the regulation of FSH secretion in the human male","volume":"86","author":"Hayes","year":"2001","journal-title":"J Clin Endocrinol Metab."},{"issue":"1","key":"2021120108562840900_CIT0005","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1053\/beem.2001.0179","article-title":"Inhibins in childhood and puberty","volume":"16","author":"Raivio","year":"2002","journal-title":"Best Pract Res Clin Endocrinol Metab."},{"issue":"3","key":"2021120108562840900_CIT0006","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1046\/j.1365-2605.2000.00229.x","article-title":"Regulation of inhibin production in the human male and its clinical applications","volume":"23","author":"Anderson","year":"2000","journal-title":"Int J Androl."},{"issue":"9073","key":"2021120108562840900_CIT0007","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0140-6736(05)62227-1","article-title":"Treatment of prepubertal gonadotrophin-deficient boys with recombinant human follicle-stimulating hormone","volume":"350","author":"Raivio","year":"1997","journal-title":"Lancet."},{"issue":"12","key":"2021120108562840900_CIT0008","first-page":"4451","article-title":"Different roles of prepubertal and postpubertal germ cells and Sertoli cells in the regulation of serum inhibin B levels","volume":"83","author":"Andersson","year":"1998","journal-title":"J Clin Endocrinol Metab."},{"issue":"1","key":"2021120108562840900_CIT0009","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1530\/eje.1.02315","article-title":"Treatment of gonadotropin-deficient boys with recombinant human FSH: long-term observation and outcome","volume":"156","author":"Raivio","year":"2007","journal-title":"Eur J Endocrinol."},{"issue":"12","key":"2021120108562840900_CIT0010","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1210\/js.2018-00225","article-title":"Recombinant human FSH treatment outcomes in five boys with severe congenital hypogonadotropic hypogonadism","volume":"2","author":"Kohva","year":"2018","journal-title":"J Endocr Soc."},{"issue":"11","key":"2021120108562840900_CIT0011","first-page":"CR511","article-title":"Plasma inhibin B and antim\u00fcllerian hormone concentrations in boys: discriminating between congenital hypogonadotropic hypogonadism and constitutional pubertal delay","volume":"16","author":"Adan","year":"2010","journal-title":"Med Sci Monit."},{"issue":"1","key":"2021120108562840900_CIT0012","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/cen.12613","article-title":"Inhibin B plus LH vs GnRH agonist test for distinguishing constitutional delay of growth and puberty from isolated hypogonadotropic hypogonadism in boys","volume":"82","author":"Binder","year":"2015","journal-title":"Clin Endocrinol (Oxf)."},{"issue":"1","key":"2021120108562840900_CIT0013","first-page":"147","article-title":"Congenital hypogonadotropic hypogonadism, functional hypogonadotropism or constitutional delay of growth and puberty? An analysis of a large patient series from a single tertiary center","volume":"32","author":"Varimo","year":"2017","journal-title":"Hum Reprod."},{"issue":"12","key":"2021120108562840900_CIT0014","doi-asserted-by":"crossref","first-page":"5225","DOI":"10.1210\/jc.2010-1535","article-title":"Baseline inhibin B and anti-Mullerian hormone measurements for diagnosis of hypogonadotropic hypogonadism (HH) in boys with delayed puberty","volume":"95","author":"Coutant","year":"2010","journal-title":"J Clin Endocrinol Metab."},{"issue":"5","key":"2021120108562840900_CIT0015","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/cen.13321","article-title":"Diagnostic utility of testosterone priming prior to dynamic tests to differentiate constitutional delay in puberty from isolated hypogonadotropic hypogonadism","volume":"86","author":"Sukumar","year":"2017","journal-title":"Clin Endocrinol (Oxf)."},{"issue":"3","key":"2021120108562840900_CIT0016","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.jpeds.2015.05.039","article-title":"Accuracy of endocrine tests for detecting hypogonadotropic hypogonadism in girls","volume":"167","author":"Binder","year":"2015","journal-title":"J Pediatr."},{"issue":"5","key":"2021120108562840900_CIT0017","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1111\/andr.12088","article-title":"Inhibin B, AMH, but not INSL3, IGF1 or DHEAS support differentiation between constitutional delay of growth and puberty and hypogonadotropic hypogonadism","volume":"3","author":"Rohayem","year":"2015","journal-title":"Andrology."},{"issue":"235","key":"2021120108562840900_CIT0018","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1136\/adc.44.235.291","article-title":"Variations in pattern of pubertal changes in girls","volume":"44","author":"Marshall","year":"1969","journal-title":"Arch Dis Child."},{"issue":"239","key":"2021120108562840900_CIT0019","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1136\/adc.45.239.13","article-title":"Variations in the pattern of pubertal changes in boys","volume":"45","author":"Marshall","year":"1970","journal-title":"Arch Dis Child."},{"key":"2021120108562840900_CIT0020","doi-asserted-by":"crossref","DOI":"10.1097\/00000441-195909000-00030","volume-title":"Radiographic Atlas of Skeletal Development of the Hand and Wrist.","author":"Greulich","year":"1959"},{"key":"2021120108562840900_CIT0021","first-page":"1","article-title":"Physical growth of Swiss children from birth to 20 years of age. First Zurich longitudinal study of growth and development","volume":"52","author":"Prader","year":"1989","journal-title":"Helv Paediatr Acta Suppl."},{"issue":"1","key":"2021120108562840900_CIT0022","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1530\/eje.0.1490023","article-title":"The human chorionic gonadotropin test is more powerful than the gonadotropin-releasing hormone agonist test to discriminate male isolated hypogonadotropic hypogonadism from constitutional delayed puberty","volume":"149","author":"Degros","year":"2003","journal-title":"Eur J Endocrinol."},{"key":"2021120108562840900_CIT0023"},{"key":"2021120108562840900_CIT0024"},{"issue":"9","key":"2021120108562840900_CIT0025","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1210\/jc.2012-1598","article-title":"Distinguishing constitutional delay of growth and puberty from isolated hypogonadotropic hypogonadism: critical appraisal of available diagnostic tests","volume":"97","author":"Harrington","year":"2012","journal-title":"J Clin Endocrinol Metab."},{"issue":"3","key":"2021120108562840900_CIT0026","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1677\/joe.0.0620573","article-title":"A priming effect of luteinizing hormone releasing factor on the anterior pituitary gland in the female rat","volume":"62","author":"Aiyer","year":"1974","journal-title":"J Endocrinol."},{"key":"2021120108562840900_CIT0027","first-page":"115","article-title":"Hypothalamic-pituitary regulation of puberty in man: evidence and concepts derived from clinical research","volume-title":"Control of the Onset of Puberty.","author":"Grumbach","year":"1974"},{"issue":"6","key":"2021120108562840900_CIT0028","doi-asserted-by":"crossref","first-page":"e271","DOI":"10.1111\/apa.12972","article-title":"New reference charts for testicular volume in Dutch children and adolescents allow the calculation of standard deviation scores","volume":"104","author":"Joustra","year":"2015","journal-title":"Acta Paediatr."},{"issue":"1","key":"2021120108562840900_CIT0029","doi-asserted-by":"crossref","first-page":"89","DOI":"10.2214\/ajr.159.1.1609728","article-title":"Ovarian cysts are common in premenarchal girls: a sonographic study of 101 children 2-12 years old","volume":"159","author":"Cohen","year":"1992","journal-title":"AJR Am J Roentgenol."},{"issue":"3","key":"2021120108562840900_CIT0030","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1210\/jc.2008-0302","article-title":"Role of gonadotropin-releasing hormone and human chorionic gonadotropin stimulation tests in differentiating patients with hypogonadotropic hypogonadism from those with constitutional delay of growth and puberty","volume":"94","author":"Segal","year":"2009","journal-title":"J Clin Endocrinol Metab."},{"issue":"8","key":"2021120108562840900_CIT0031","doi-asserted-by":"crossref","first-page":"e2717","DOI":"10.1210\/clinem\/dgaa162","article-title":"Using kisspeptin to predict pubertal outcomes for youth with pubertal delay","volume":"105","author":"Chan","year":"2020","journal-title":"J Clin Endocrinol Metab."},{"key":"2021120108562840900_CIT0032","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1046\/j.1365-2265.1999.00814.x","article-title":"Sexual dimorphism of monomeric and dimeric inhibins in normal boys and girls from birth to puberty","volume":"51","author":"Bergada","year":"1999","journal-title":"Clin Endocrinol."}],"container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/advance-article-pdf\/doi\/10.1210\/clinem\/dgab357\/38852711\/dgab357.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/106\/9\/e3495\/41379321\/dgab357.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/106\/9\/e3495\/41379321\/dgab357.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T14:01:30Z","timestamp":1638367290000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/106\/9\/e3495\/6278376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,19]]},"references-count":32,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,5,19]]},"published-print":{"date-parts":[[2021,8,18]]}},"URL":"https:\/\/doi.org\/10.1210\/clinem\/dgab357","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,9,1]]},"published":{"date-parts":[[2021,5,19]]}}}