{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T20:47:03Z","timestamp":1770842823240,"version":"3.50.1"},"reference-count":45,"publisher":"The Endocrine Society","issue":"4","funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1-DK088114"],"award-info":[{"award-number":["RO1-DK088114"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific and Technical Research Council of Turkey","award":["TUBITAK-BIDEB: 2211-A"],"award-info":[{"award-number":["TUBITAK-BIDEB: 2211-A"]}]},{"name":"Turkish Council of Higher Education","award":["Y\u00d6K 100\/2000"],"award-info":[{"award-number":["Y\u00d6K 100\/2000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,24]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Context<\/jats:title><jats:p>Leptin replacement with metreleptin improves glycemia and hypertriglyceridemia in severely hypoleptinemic patients with generalized lipodystrophy (GLD), but its effects are variable in partially leptin-deficient patients with partial lipodystrophy (PLD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Compare 3 leptin assays (Study I); identify diagnostic performance of leptin assays to detect responders to metreleptin for each assay (Study II).<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Study I: cross-sectional analysis of average bias between leptin assays. Study II: retrospective analysis of diagnostic accuracy of potential leptin cut points to detect clinical responders to metreleptin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>National Institutes of Health; University of Michigan.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants and Interventions<\/jats:title><jats:p>Study I: Metreleptin-na\u00efve patients with lipodystrophy (GLD, n = 33, PLD, n = 67) and healthy volunteers (n = 239). Study II: GLD (n = 66) and PLD (n = 84) patients treated with metreleptin for 12 months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Outcome Measures<\/jats:title><jats:p>Leptin concentrations by Millipore radioimmunoassay (RIA), Millipore enzyme-linked immunosorbent assay (MELISA), and R&amp;D Systems enzyme-linked immunosorbent assay (RDELISA). Response to metreleptin therapy was defined as either reduction \u22651.0% in A1c or \u226530% in serum triglycerides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RDELISA measured 3.0 \u00b1 9.5 ng\/mL higher than RIA; MELISA measured 11.0 \u00b1 17.8 and 14.0 \u00b119.2 less than RIA and RDELISA, respectively. Leptin by RIA, MELISA, and RDELISA modestly predicted metreleptin response in GLD + PLD [receiver operating characteristic (ROC) area under the curve (AUC) 0.74, 0.69, and 0.71, respectively; P &amp;lt; 0.01 for all] with lower predictive power in PLD (ROC AUC 0.63, 0.61 and 0.65, respectively; P &amp;gt; 0.05 for all). The only reproducible cut point identified on sensitivity analyses was RIA leptin 7.2 ng\/mL (sensitivity 56%; specificity 78%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Three common leptin assays are not interchangeable, and a reliable cut point to select responders to metreleptin was not identified.<\/jats:p><\/jats:sec>","DOI":"10.1210\/clinem\/dgab760","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T13:51:05Z","timestamp":1634910665000},"page":"e1739-e1751","source":"Crossref","is-referenced-by-count":17,"title":["Endogenous Leptin Concentrations Poorly Predict Metreleptin Response in Patients With Partial Lipodystrophy"],"prefix":"10.1210","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-5049","authenticated-orcid":false,"given":"Rasimcan","family":"Meral","sequence":"first","affiliation":[{"name":"Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and 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