{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T14:21:34Z","timestamp":1782570094024,"version":"3.54.5"},"reference-count":29,"publisher":"The Endocrine Society","issue":"9","funder":[{"name":"German Federal Ministry of Economics and Technology","award":["KF2257304AJ0"],"award-info":[{"award-number":["KF2257304AJ0"]}]},{"name":"ZIM program"},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Context<\/jats:title>\n                  <jats:p>Severe insulin resistance (IR) in the presence of insulin receptor autoantibodies (InsR-aAb) is known as type B insulin resistance (TBIR). Considerable progress in therapy has been achieved, but diagnosis and monitoring of InsR-aAb remains a challenge.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>This work aimed to establish a robust in vitro method for InsR-Ab quantification.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Longitudinal serum samples from patients with TBIR at the National Institutes of Health were collected. A bridge-assay for InsR-aAb detection was established using recombinant human insulin receptor as bait and detector. Monoclonal antibodies served as positive controls for validation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The novel assay proved sensitive, robust, and passed quality control. The measured InsR-aAb from TBIR patients was associated with disease severity, decreased on treatment, and inhibited insulin signaling in vitro. Titers of InsR-aAb correlated positively to fasting insulin in patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Quantification of InsR-aAb from serum samples via the novel in vitro assay enables identification of TBIR and monitoring of successful therapy.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/clinem\/dgad125","type":"journal-article","created":{"date-parts":[[2023,3,4]],"date-time":"2023-03-04T11:16:06Z","timestamp":1677928566000},"page":"2324-2329","source":"Crossref","is-referenced-by-count":10,"title":["A Novel In Vitro Assay Correlates Insulin Receptor Autoantibodies With Fasting Insulin in Type B Insulin Resistance"],"prefix":"10.1210","volume":"108","author":[{"given":"Waldemar B","family":"Minich","sequence":"first","affiliation":[{"name":"Institute for Experimental Endocrinology, Max Rubner Center (MRC) for Cardiovascular Metabolic Renal Research, Charit\u00e9-Universit\u00e4tsmedizin Berlin , D-10115, Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3598-0542","authenticated-orcid":false,"given":"Brent S","family":"Abel","sequence":"additional","affiliation":[{"name":"National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases , Bethesda, MD 20892 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Schwiebert","sequence":"additional","affiliation":[{"name":"Institute for Experimental Endocrinology, Max Rubner Center (MRC) for Cardiovascular Metabolic Renal Research, Charit\u00e9-Universit\u00e4tsmedizin Berlin , D-10115, Berlin , Germany"},{"name":"selenOmed GmbH , D-10965, Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Welsink","sequence":"additional","affiliation":[{"name":"Institute for Experimental Endocrinology, Max Rubner Center (MRC) for Cardiovascular Metabolic Renal Research, Charit\u00e9-Universit\u00e4tsmedizin Berlin , D-10115, Berlin , Germany"},{"name":"selenOmed GmbH , D-10965, Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Seemann","sequence":"additional","affiliation":[{"name":"Institute for Experimental Endocrinology, Max Rubner Center (MRC) for Cardiovascular Metabolic Renal Research, Charit\u00e9-Universit\u00e4tsmedizin Berlin , D-10115, Berlin , Germany"},{"name":"InVivo BioTech Services GmbH , D-16761, Hennigsdorf , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2589-7382","authenticated-orcid":false,"given":"Rebecca J","family":"Brown","sequence":"additional","affiliation":[{"name":"National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases , Bethesda, MD 20892 , 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