{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:38:16Z","timestamp":1792460296583,"version":"4.5.0"},"reference-count":29,"publisher":"The Endocrine Society","issue":"2","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100011470","name":"Shanghai Minhang Science and Technology Commission","doi-asserted-by":"publisher","award":["2019MHZ042"],"award-info":[{"award-number":["2019MHZ042"]}],"id":[{"id":"10.13039\/501100011470","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020208","name":"Shanghai Minhang District Health Commission","doi-asserted-by":"publisher","award":["2020MZYS04"],"award-info":[{"award-number":["2020MZYS04"]}],"id":[{"id":"10.13039\/501100020208","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["2022YQ033"],"award-info":[{"award-number":["2022YQ033"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270852"],"award-info":[{"award-number":["82270852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["21ZR1450500"],"award-info":[{"award-number":["21ZR1450500"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Placenta-derived inflammation plays a vital role in the pathophysiology of gestational diabetes mellitus (GDM). IL-32 is a novel pro-inflammatory cytokine and metabolic regulator involved in the development of metabolic disease. We investigated the effect of IL-32 in GDM.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and Methods<\/jats:title>\n                  <jats:p>First-trimester C-reactive protein (CRP) level was monitored in a case-control study of 186 women with GDM and 186 women without. Placental tissue was lysed and analyzed by high-resolution liquid chromatography-tandem mass spectrometry. Circulating level of inflammatory cytokines IL-32, IL-6, and TNF-\u03b1 were measured by ELISA kits. The expression of placenta-derived macrophages, inflammatory cytokines, and related pathway proteins were assessed by reverse transcriptase-quantitative PCR, western blot, immunohistochemistry, or immunofluorescence.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>First-trimester CRP level in peripheral blood was closely associated with glucose and insulin resistance index and was an independent correlation with the development of GDM. High-resolution liquid chromatography-tandem mass spectrometry revealed that placenta-derived CRP expression was dramatically elevated in women with GDM. Interestingly, the expression of placenta-derived IL-32 was also increased and located in the macrophages of placental tissue. Meanwhile, the expression of IL-6, TNF-\u03b1, and p-p38 were up-regulated in the placental tissues with GDM. Either IL-6 or TNF-\u03b1 was colocated with IL-32 in the placental tissue. Importantly, circulating IL-32 throughout pregnancy was increased in GDM and was related to placental-derived IL-32 expression, circulating IL-6, and TNF-\u03b1, glucose and insulin resistance index.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Increased circulating IL-32 throughout pregnancy was closely associated with placenta macrophage-derived IL-32 expression and GDM. First trimester IL-32 level in peripheral blood may serve to predict the development of GDM.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/clinem\/dgad531","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T22:17:31Z","timestamp":1694729851000},"page":"333-343","source":"Crossref","is-referenced-by-count":12,"title":["Increased Circulating IL-32 Is Associated With Placenta Macrophage-derived IL-32 and Gestational Diabetes Mellitus"],"prefix":"10.1210","volume":"109","author":[{"given":"Xinmei","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Shanghai Fifth People\u2019s Hospital, Fudan University , Shanghai 200240 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Shanghai Fifth People\u2019s Hospital, Fudan University , Shanghai 200240 , 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