{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:52:30Z","timestamp":1791337950528,"version":"4.3.1"},"reference-count":31,"publisher":"The Endocrine Society","issue":"5","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000862","name":"Doris Duke Foundation","doi-asserted-by":"publisher","award":["1R01AG080516"],"award-info":[{"award-number":["1R01AG080516"]}],"id":[{"id":"10.13039\/100000862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute of Aging","doi-asserted-by":"publisher","award":["1R01HL15583401"],"award-info":[{"award-number":["1R01HL15583401"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Context<\/jats:title>\n                  <jats:p>Androstenedione (A4) and testosterone (T) are produced by both the adrenal glands and the gonads. The adrenal enzyme 11\u03b2-hydroxylase (CYP11B1) executes the final step in cortisol synthesis; CYP11B1 also uses A4 and T as substrates, generating 11-hydroxyandrostenedione and 11-hydroxytestosterone, respectively. It has been suggested that CYP11B1 is expressed in the gonads, yet the circulating levels of all 11-oxygenated androgens (11-oxyandrogens) are similar in males and females of reproductive ages, despite enormous differences in T.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To assess the gonadal contribution to the circulating pool of 11-oxyandrogens.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We used liquid chromatography\u2013tandem mass spectrometry to measure 13 steroids, including traditional and 11-oxyandrogens in: (i) paired gonadal and peripheral vein blood samples obtained during gonadal venograms from 11 patients (7 women), median age 37 (range, 31-51 years); and (ii) 17 women, median age 57 (range, 41-81 years) before and after bilateral salpingo-oophorectomy (BSO). We also compared CYP11B1, 17\u03b1-hydroxylase\/17,20-lyase (CYP17A1), and 3\u03b2-hydroxysteroid dehydrogenase type 2 (HSD3B2) mRNA expression in adrenal, ovarian, and testicular tissue.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A4, T, estradiol, estrone, progesterone, 17\u03b1- and 16\u03b1-hydroxyprogesterone were all higher in gonadal veins vs periphery (P &amp;lt; .05 for all), while four 11-oxyandrogens were similar between matched gonadal and peripheral vein samples. Equally, in women who underwent BSO, A4 (median [interquartile range]: 59.7 [47.7-67.6] ng\/dL vs 32.7 [27.4-47.8] ng\/dL, P &amp;lt; .001) and T (24.1 [16.4-32.3] vs 15.5 [13.7-19.0] ng\/dL, P &amp;lt; .001) declined, while 11-oxyandrogens remained stable. Gonadal tissue displayed negligible CYP11B1 mRNA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Despite producing substantial amounts of A4 and T, human gonads are not relevant sources of 11-oxyandrogens.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/clinem\/dgae420","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T05:25:29Z","timestamp":1718601929000},"page":"1398-1403","source":"Crossref","is-referenced-by-count":12,"title":["Human Gonads Do Not Contribute to the Circulating Pool of 11-Oxygenated Androgens"],"prefix":"10.1210","volume":"110","author":[{"given":"Suranut","family":"Charoensri","sequence":"first","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]},{"name":"Division of Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine, Khon Kaen University , Khon Kaen 40000 ,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juilee","family":"Rege","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaelin","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xhorlina","family":"Marko","sequence":"additional","affiliation":[{"name":"Division of Vascular and Interventional Radiology, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Sherk","sequence":"additional","affiliation":[{"name":"Division of Vascular and Interventional Radiology, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta","family":"Sholinyan","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E","family":"Rainey","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9831-6190","authenticated-orcid":false,"given":"Adina F","family":"Turcu","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan , Ann Arbor, MI 48109 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"issue":"9","key":"2025042208272098200_dgae420-B1","first-page":"3078","article-title":"Prevalence of the polycystic ovary syndrome in unselected black and white women of the southeastern United States: a prospective study","volume":"83","author":"Knochenhauer","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"issue":"4","key":"2025042208272098200_dgae420-B2","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1093\/humrep\/des028","article-title":"Prevalence of functional disorders of androgen excess in unselected premenopausal women: a study in blood 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