{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:20:49Z","timestamp":1787728849244,"version":"build-2784847793"},"reference-count":43,"publisher":"The Endocrine Society","issue":"6","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000041","name":"American Diabetes Association","doi-asserted-by":"publisher","award":["7-12-CT-40"],"award-info":[{"award-number":["7-12-CT-40"]}],"id":[{"id":"10.13039\/100000041","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Mayo Clinic Metabolomics Resource Core","award":["U24DK100469"],"award-info":[{"award-number":["U24DK100469"]}]},{"DOI":"10.13039\/100000002","name":"National Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000871","name":"Mayo Clinic CTSA","doi-asserted-by":"publisher","award":["UL1TR000135"],"award-info":[{"award-number":["UL1TR000135"]}],"id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Humans with obesity and insulin resistance exhibit lipid accumulation in skeletal muscle, but the underlying biological mechanisms responsible for the accumulation of lipid in the muscle of these individuals remain unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We investigated how plasma insulin modulates the extraction of circulating triglycerides (TGs) and nonesterified fatty acids (NEFAs) from ingested and endogenous origin in the muscle of lean, insulin-sensitive humans (Lean-IS) and contrasted these responses to those in humans with obesity and insulin resistance (Obese-IR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The studies were performed in a postprandial state associated with steady-state plasma TG concentrations. The arterio-venous blood sampling technique was employed to determine the extraction of circulating lipids across the forearm muscle before and after insulin infusion. We distinguished the kinetics of TGs and NEFAs from ingested origin from those from endogenous origin across muscle by incorporating stable isotope-labeled triolein in the ingested fat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Insulin infusion rapidly suppressed the extraction of plasma TGs from endogenous but not ingested origin in the muscle of the Lean-IS, but this response was absent in the muscle of the Obese-IR. Furthermore, in the muscle of the Lean-IS, insulin infusion decreased the extraction of circulating NEFAs from both ingested and endogenous origin; however, this response was absent for NEFAs from ingested origin in the muscle of the Obese-IR subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Partitioning of circulating lipids away from the skeletal muscle when plasma insulin increases during the postprandial period is impaired in humans with obesity and insulin resistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/clinem\/dgae727","type":"journal-article","created":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T15:13:00Z","timestamp":1728832380000},"page":"1647-1656","source":"Crossref","is-referenced-by-count":2,"title":["Impaired Suppression of Plasma Lipid Extraction and Its Partitioning Away From Muscle by Insulin in Humans With Obesity"],"prefix":"10.1210","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3025-5752","authenticated-orcid":false,"given":"Christos S","family":"Katsanos","sequence":"first","affiliation":[{"name":"School of Life Sciences, Arizona State University , Tempe, AZ 85259 ,","place":["USA"]},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic Arizona , Scottsdale, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Tran","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University , Tempe, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nyssa","family":"Hoffman","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University , Tempe, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori R","family":"Roust","sequence":"additional","affiliation":[{"name":"College of Medicine, Mayo Clinic Arizona , Scottsdale, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanna","family":"De Filippis","sequence":"additional","affiliation":[{"name":"College of Medicine, Mayo Clinic Arizona , Scottsdale, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence J","family":"Mandarino","sequence":"additional","affiliation":[{"name":"Department of Medicine, and Center for Disparities in Diabetes, Obesity, and Metabolism, University of Arizona College of Medicine , Tucson, AZ 85724 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailin","family":"Johnsson","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University , Tempe, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Belohlavek","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases, Mayo Clinic Arizona , Scottsdale, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R","family":"Buras","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences, Mayo Clinic Arizona , Scottsdale, AZ 85259 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"issue":"4","key":"2025052712582081200_dgae727-B1","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.2337\/diabetes.51.4.1022","article-title":"Assessment of skeletal muscle triglyceride content by (1)H nuclear magnetic resonance spectroscopy in lean and obese adolescents: relationships to insulin sensitivity, total body fat, and central 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