{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T11:52:01Z","timestamp":1782301921009,"version":"3.54.5"},"reference-count":34,"publisher":"The Endocrine Society","issue":"1","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173579"],"award-info":[{"award-number":["82173579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872671"],"award-info":[{"award-number":["81872671"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["21ZR1403800"],"award-info":[{"award-number":["21ZR1403800"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Taizhou City Foundation for Talents","award":["TZ2022-2"],"award-info":[{"award-number":["TZ2022-2"]}]},{"name":"Yiwu Research Institute of Fudan University","award":["KCF201512"],"award-info":[{"award-number":["KCF201512"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Lipid metabolism plays an important role in HIV-associated atherosclerosis. However, the interplay between lipid metabolism, uncontrolled inflammation, and subclinical carotid atherosclerosis (SCA) in HIV infection remains poorly understood. This study aimed to characterize lipidome signatures that, together with inflammatory patterns, may predispose HIV-positive individuals to incident SCA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A nested case-control study was conducted within the Comparative HIV and Aging Research Cohort in Taizhou, China, including 115 HIV-positive individuals with incident SCA, 112 age- and sex-comparable HIV-positive normal controls, and 117 HIV-negative normal controls. Untargeted lipidomic profiling and inflammatory marker assessments were performed at baseline and follow-up. A total of 649 plasma lipid species were annotated, and 20 plasma inflammatory markers were quantified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three distinct baseline lipidomic signatures were identified: 1 upregulated in HIV infection, 1 downregulated, and 1 upregulated in HIV-associated SCA (showing a decreasing or increasing trend from HIV-negative normal controls to HIV-positive normal controls to SCA cases). The latter 2 signatures were enriched in glycerophospholipid metabolism, particularly involving lysophospholipids and short-chain fatty acyls. Lipid species within each signature exhibited distinct correlation patterns with subsets of inflammatory proteins, a pattern that persisted after the onset of SCA. Network analysis revealed that IL-18 was the only inflammatory protein showing divergent associations across lipidomic signatures, displaying positive correlations in the HIV-associated SCA-upregulated lipidomic signature and negative correlations in the other 2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our findings underscore specific lipidomic-inflammatory networks that may underlie the heightened risk of atherosclerosis in HIV infection. The differential involvement of IL-18 suggests a central role of NLRP3 inflammasome activation in HIV-associated vascular inflammation. The observed alterations in lysophospholipid and short-chain fatty acyl metabolism warrant further mechanistic investigation as potential mediators of HIV-related atherogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/clinem\/dgaf331","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T07:54:41Z","timestamp":1749196481000},"page":"62-71","source":"Crossref","is-referenced-by-count":2,"title":["Inflammation-associated Lipidomic Signatures Prior to Carotid Artery Atherosclerosis in People Living With HIV"],"prefix":"10.1210","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6533-8035","authenticated-orcid":false,"given":"Yingying","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]},{"name":"Yi-Wu Research Institute, Fudan University , Yiwu 322000, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]},{"name":"Department of AIDS\/STD Prevention and Control, Taizhou City Center for Disease Control and Prevention , Taizhou 318000, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of AIDS\/STD Prevention and Control, Taizhou City Center for Disease Control and Prevention , Taizhou 318000, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunqiu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaochen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of AIDS\/STD Prevention and Control, Taizhou City Center for Disease Control and Prevention , Taizhou 318000, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5560-5312","authenticated-orcid":false,"given":"Na","family":"He","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety of Ministry of Education, Fudan University , Shanghai 200032 ,","place":["China"]},{"name":"Yi-Wu Research Institute, Fudan University , Yiwu 322000, Zhejiang Province ,","place":["China"]},{"name":"Shanghai Institute of Infectious Diseases and Biosecurity, Fudan University , Shanghai 200032 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2025,6,9]]},"reference":[{"issue":"6","key":"2025121807424416800_dgaf331-B1","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1111\/hiv.13077","article-title":"The burden of non-communicable diseases and mortality in people living with HIV (PLHIV) in the pre-, early- and late-HAART era","volume":"22","author":"Jespersen","year":"2021","journal-title":"HIV Med"},{"issue":"11","key":"2025121807424416800_dgaf331-B2","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1161\/CIRCRESAHA.124.323891","article-title":"Inflammation in HIV and its impact on atherosclerotic cardiovascular disease","volume":"134","author":"Obare","year":"2024","journal-title":"Circ Res"},{"issue":"10","key":"2025121807424416800_dgaf331-B3","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1093\/infdis\/jiz351","article-title":"Immune activation and inflammation in people with human immunodeficiency virus: challenging targets","volume":"221","author":"Utay","year":"2020","journal-title":"J Infect Dis"},{"issue":"14","key":"2025121807424416800_dgaf331-B4","doi-asserted-by":"crossref","first-page":"e158448","DOI":"10.1172\/JCI158448","article-title":"Connecting aging biology and inflammation in the omics era","volume":"132","author":"Walker","year":"2022","journal-title":"J Clin Invest"},{"issue":"1","key":"2025121807424416800_dgaf331-B5","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41577-020-0381-7","article-title":"Immunometabolism and HIV-1 pathogenesis: food for thought","volume":"21","author":"S\u00e1ez-Ciri\u00f3n","year":"2021","journal-title":"Nat Rev Immunol"},{"issue":"12","key":"2025121807424416800_dgaf331-B6","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1194\/jlr.R066514","article-title":"Lipid signaling and lipotoxicity in metaflammation: indications for metabolic disease pathogenesis and treatment","volume":"57","author":"Ertunc","year":"2016","journal-title":"J Lipid Res"},{"issue":"12","key":"2025121807424416800_dgaf331-B7","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1001\/jamacardio.2019.4025","article-title":"Association of lipidomic profiles with progression of carotid artery atherosclerosis in HIV infection","volume":"4","author":"Chai","year":"2019","journal-title":"JAMA Cardiol"},{"key":"2025121807424416800_dgaf331-B8","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ebiom.2017.07.015","article-title":"Immunometabolic and lipidomic markers associated with the frailty index and quality of life in aging HIV+ men on antiretroviral therapy","volume":"22","author":"Yeoh","year":"2017","journal-title":"EBioMedicine"},{"issue":"2","key":"2025121807424416800_dgaf331-B9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s11904-020-00484-4","article-title":"Dysfunctional immunometabolism in HIV infection: contributing factors and implications for age-related comorbid diseases","volume":"17","author":"Butterfield","year":"2020","journal-title":"Curr HIV\/AIDS Rep"},{"key":"2025121807424416800_dgaf331-B10","doi-asserted-by":"crossref","first-page":"103548","DOI":"10.1016\/j.ebiom.2021.103548","article-title":"Comprehensive metabolomics profiling reveals common metabolic alterations underlying the four major non-communicable diseases in treated HIV infection","volume":"71","author":"Ding","year":"2021","journal-title":"EBioMedicine"},{"issue":"12","key":"2025121807424416800_dgaf331-B11","doi-asserted-by":"crossref","first-page":"e860","DOI":"10.1016\/S2352-3018(19)30263-2","article-title":"Age-specific associations between HIV infection and carotid artery intima-media thickness in China: a cross-sectional evaluation of baseline data from the CHART cohort","volume":"6","author":"Lin","year":"2019","journal-title":"Lancet HIV"},{"issue":"4","key":"2025121807424416800_dgaf331-B12","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/ije\/dyp233","article-title":"Cohort profile: the Chinese national free antiretroviral treatment cohort","volume":"39","author":"Ma","year":"2010","journal-title":"Int J Epidemiol"},{"key":"2025121807424416800_dgaf331-B13","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1600-0404.2009.01210.x","article-title":"Ultrasonographic measurements of subclinical carotid atherosclerosis in prediction of ischemic stroke","volume":"189","author":"Mathiesen","year":"2009","journal-title":"Acta Neurol Scand"},{"key":"2025121807424416800_dgaf331-B14","doi-asserted-by":"crossref","unstructured":"Ding Y . 2025. Supplementary material for \u201cInflammation-associated lipidomic signatures prior to carotid artery atherosclerosis in people living with HIV.\u201d \u00a0Zenodo. 10.5281\/zenodo.15477585.","DOI":"10.1210\/clinem\/dgaf331"},{"issue":"9","key":"2025121807424416800_dgaf331-B15","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.3390\/nu12092555","article-title":"Expert opinion on benefits of long-chain omega-3 fatty acids (DHA and EPA) in aging and clinical nutrition","volume":"12","author":"Troesch","year":"2020","journal-title":"Nutrients"},{"issue":"6","key":"2025121807424416800_dgaf331-B16","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1089\/jmf.2020.0119","article-title":"Decreased docosahexaenoic acid levels in serum of HIV carrier patients","volume":"24","author":"Godoy","year":"2020","journal-title":"J Med Food"},{"issue":"17","key":"2025121807424416800_dgaf331-B17","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1097\/QAD.0000000000001238","article-title":"Oxidized lipoproteins are associated with markers of inflammation and immune activation in HIV-1 infection","volume":"30","author":"Kelesidis","year":"2016","journal-title":"AIDS"},{"issue":"12","key":"2025121807424416800_dgaf331-B18","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.3390\/ijms21124501","article-title":"An updated review of pro- and anti-inflammatory properties of plasma lysophosphatidylcholines in the vascular system","volume":"21","author":"Knuplez","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"1-2","key":"2025121807424416800_dgaf331-B19","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s11010-006-9280-x","article-title":"Lysophosphatidylcholine induces inflammatory activation of human coronary artery smooth muscle cells","volume":"295","author":"Aiyar","year":"2007","journal-title":"Mol Cell Biochem"},{"issue":"17","key":"2025121807424416800_dgaf331-B20","doi-asserted-by":"crossref","first-page":"e121326","DOI":"10.1172\/jci.insight.121326","article-title":"Large-scale plasma lipidomic profiling identifies lipids that predict cardiovascular events in secondary prevention","volume":"3","author":"Mundra","year":"2018","journal-title":"JCI Insight"},{"issue":"6","key":"2025121807424416800_dgaf331-B21","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/j.mehy.2015.10.013","article-title":"Fatty acyl composition of lysophosphatidylcholine is important in atherosclerosis","volume":"85","author":"Akerele","year":"2015","journal-title":"Med Hypotheses"},{"issue":"3","key":"2025121807424416800_dgaf331-B22","doi-asserted-by":"crossref","first-page":"376","DOI":"10.20411\/pai.v2i3.218","article-title":"Prospective analysis of lipid composition changes with antiretroviral therapy and immune activation in persons living with HIV","volume":"2","author":"Belury","year":"2017","journal-title":"Pathog Immun"},{"issue":"4","key":"2025121807424416800_dgaf331-B23","doi-asserted-by":"crossref","first-page":"e94810","DOI":"10.1371\/journal.pone.0094810","article-title":"Plasma lipidomic profiling of treated HIV-positive persons and the implications for cardiovascular risk prediction","volume":"9","author":"Wong","year":"2014","journal-title":"PLoS One"},{"issue":"21","key":"2025121807424416800_dgaf331-B24","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1161\/CIRCULATIONAHA.116.023233","article-title":"Plasma lipidomic profiles improve on traditional risk factors for the prediction of cardiovascular events in type 2 diabetes mellitus","volume":"134","author":"Alshehry","year":"2016","journal-title":"Circulation"},{"issue":"11","key":"2025121807424416800_dgaf331-B25","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1038\/nrendo.2016.98","article-title":"Lipidomics for studying metabolism","volume":"12","author":"Han","year":"2016","journal-title":"Nat Rev Endocrinol"},{"issue":"3","key":"2025121807424416800_dgaf331-B26","doi-asserted-by":"crossref","first-page":"930","DOI":"10.3945\/ajcn.117.156265","article-title":"Short-chain fatty acids, prebiotics, synbiotics, and systemic inflammation: a systematic review and meta-analysis","volume":"106","author":"McLoughlin","year":"2017","journal-title":"Am J Clin Nutr"},{"issue":"11","key":"2025121807424416800_dgaf331-B27","doi-asserted-by":"crossref","first-page":"113336","DOI":"10.1016\/j.celrep.2023.113336","article-title":"Impaired gut microbiota-mediated short-chain fatty acid production precedes morbidity and mortality in people with HIV","volume":"42","author":"Giera","year":"2023","journal-title":"Cell Rep"},{"issue":"5","key":"2025121807424416800_dgaf331-B28","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.molmed.2022.02.010","article-title":"Role of inflammasomes in HIV-1 infection and treatment","volume":"28","author":"Jin","year":"2022","journal-title":"Trends Mol Med"},{"key":"2025121807424416800_dgaf331-B29","doi-asserted-by":"crossref","first-page":"109013","DOI":"10.1016\/j.clim.2022.109013","article-title":"The critical role of short-chain fatty acids in health and disease: a subtle focus on cardiovascular disease-NLRP3 inflammasome-angiogenesis axis","volume":"238","author":"Yao","year":"2022","journal-title":"Clin Immunol"},{"key":"2025121807424416800_dgaf331-B30","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jneuroim.2017.07.004","article-title":"Lysophosphatidylcholine activates caspase-1 in microglia via a novel pathway involving two inflammasomes","volume":"310","author":"Scholz","year":"2017","journal-title":"J Neuroimmunol"},{"issue":"4","key":"2025121807424416800_dgaf331-B31","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.molcel.2012.04.026","article-title":"A perspective on mammalian caspases as positive and negative regulators of inflammation","volume":"46","author":"Martin","year":"2012","journal-title":"Mol Cell"},{"issue":"6581","key":"2025121807424416800_dgaf331-B32","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1126\/science.abg7292","article-title":"Caloric restriction in humans reveals immunometabolic regulators of health span","volume":"375","author":"Spadaro","year":"2022","journal-title":"Science"},{"issue":"9","key":"2025121807424416800_dgaf331-B33","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1002\/1521-4141(200209)32:9<2635::AID-IMMU2635>3.0.CO;2-N","article-title":"Enhanced production of IL-18 in butyrate-treated intestinal epithelium by stimulation of the proximal promoter region","volume":"32","author":"Kalina","year":"2002","journal-title":"Eur J Immunol"},{"issue":"4","key":"2025121807424416800_dgaf331-B34","doi-asserted-by":"crossref","first-page":"730","DOI":"10.3945\/an.115.011387","article-title":"Pentadecanoic and heptadecanoic acids: multifaceted odd-chain fatty acids","volume":"7","author":"Pfeuffer","year":"2016","journal-title":"Adv Nutr"}],"container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jcem\/advance-article-pdf\/doi\/10.1210\/clinem\/dgaf331\/63457610\/dgaf331.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/111\/1\/62\/63457610\/dgaf331.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jcem\/article-pdf\/111\/1\/62\/63457610\/dgaf331.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T12:43:31Z","timestamp":1766061811000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/111\/1\/62\/8159007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,9]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,6,9]]},"published-print":{"date-parts":[[2025,12,18]]}},"URL":"https:\/\/doi.org\/10.1210\/clinem\/dgaf331","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,1]]},"published":{"date-parts":[[2025,6,9]]}}}