{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T17:06:11Z","timestamp":1784567171783,"version":"3.55.0"},"reference-count":59,"publisher":"The Endocrine Society","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o"},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017014","name":"CINTESIS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"name":"R&D Unit"},{"name":"CHRC"},{"name":"Projetos de investiga\u00e7\u00e3o cl\u00ednica"},{"DOI":"10.13039\/501100013175","name":"European Society for Clinical Investigation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013175","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Obesity-related metabolic complications are especially driven by adipose tissue dysfunction. Gut dysbiosis and impaired intestinal barrier function facilitate microbial fragment translocation, potentially promoting metabolic impairment. However, the presence and functional impact of microbial DNA in adipose tissue and its contribution to metabolic dysfunction remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the role of gut microbiota, intestinal barrier function, and adipose tissue-associated microbial DNA in obesity-related metabolic dysfunction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Exploratory cross-sectional study with complementary mechanistic in vitro experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Academic tertiary referral center in Lisbon, Portugal.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>Fifty adults with severe obesity undergoing laparoscopic Roux-en-Y gastric bypass surgery were recruited and stratified according to metabolic phenotype into preclinical obesity and clinical obesity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measures<\/jats:title>\n                    <jats:p>Gut microbiota composition, intestinal barrier markers, adipose tissue-associated microbial DNA profiles, and adipocyte functional responses to adipose tissue-derived microbial DNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Clinical obesity was associated with higher protein intake, impaired gut barrier function, and enrichment of hydrogen sulfide\u2013producing genera (Bilophila, Desulfovibrio), whereas preclinical obesity showed higher Akkermansia and Bifidobacterium. Adipose tissue from clinical obesity contained more microbial DNA, with reduced diversity and partial overlap with gut profiles. Functionally, adipose-derived microbial DNA from clinical obesity induced increased oxidative stress, IL-1\u03b2, and NF\u03baB expression, and a lower adiponectin\/leptin gene expression ratio in adipocytes compared with preclinical obesity DNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Clinical obesity is characterized by distinct gut microbiota signatures, impaired intestinal barrier function, and increased adipose-associated microbial DNA. Adipose tissue-derived DNA promoted adipocyte dysfunction in vitro, supporting a potential role for the gut\u2013adipose tissue microbial axis in obesity-related metabolic complications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/clinem\/dgag246","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:43:19Z","timestamp":1782214999000},"source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the role of adipose tissue microbial DNA in obesity-related metabolic dysfunction"],"prefix":"10.1210","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7028-4756","authenticated-orcid":false,"given":"Sh\u00e1mila","family":"Ismael","sequence":"first","affiliation":[{"name":"Nutrition & Metabolism, NOVA Medical School, Faculdade de Ci\u00eancias M\u00e9dicas, NMS, FCM, Universidade NOVA de Lisboa , Lisboa 1169-056 ,","place":["Portugal"]},{"name":"CHRC, NOVA Medical School, Faculdade de Ci\u00eancias M\u00e9dicas, NMS, FCM, Universidade 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