{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T15:14:31Z","timestamp":1783350871778,"version":"3.54.6"},"reference-count":22,"publisher":"The Endocrine Society","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Oral estrogens raise corticosteroid-binding globulin (CBG) levels, leading to increased serum total cortisol concentrations. Guidelines advise discontinuation at least 6 weeks before performing endocrine function tests measuring total serum cortisol concentrations for evaluation of hypothalamic-pituitary-adrenal function in women using combined oral contraceptives (COC). The exact rate of normalization of CBG is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to identify the duration for CBG and total cortisol levels to normalize after stopping COC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design, setting, and participants<\/jats:title>\n                    <jats:p>This prospective observational study included 24 healthy adult women discontinuing COC. Eight weekly fasting morning blood samples were collected, starting 1 week before to 6 weeks after cessation. CBG and total cortisol concentrations were measured using immunoassays. Data are reported as mean (SD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CBG concentrations were 133 \u00b1 20\u2005mg\/L at baseline and 59 \u00b1 10\u2005mg\/L 6 weeks after stopping COC. Total cortisol concentrations were 855 \u00b1 138\u2005nmol\/L at baseline and 370 \u00b1 130\u2005nmol\/L 6 weeks after stopping COC. CBG and cortisol concentrations showed an immediate, exponentially shaped decrease after cessation of the COC. The percentage of baseline after 4 weeks was 49 \u00b1 12% for CBG and 46 \u00b1 11% for total cortisol. The linear mixed model and piecewise linear mixed model showed nonsignificant changes after 4 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CBG and total cortisol concentrations decreased over time following discontinuation of COC. Concentrations of both CBG and total cortisol had normalized by 4 weeks after discontinuation. Accordingly, the minimum period for discontinuation of COC prior to evaluating hypothalamic-pituitary-adrenal axis measurements may be reduced from 6 weeks to 4 weeks.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/clinem\/dgag249","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:43:18Z","timestamp":1782214998000},"source":"Crossref","is-referenced-by-count":0,"title":["Prospective evaluation of the effect of stopping estrogen-containing contraceptives on serum CBG and cortisol levels"],"prefix":"10.1210","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8363-5046","authenticated-orcid":false,"given":"Karlijn","family":"Koops","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Endocrine Laboratory, Amsterdam UMC, Vrije Universiteit Amsterdam , 1081 HV Amsterdam ,","place":["The Netherlands"]},{"name":"Amsterdam Gastroenterology, Endocrinology & Metabolism , 1105 AZ Amsterdam ,","place":["The Netherlands"]},{"name":"Department of Laboratory Medicine, Endocrine Laboratory, Amsterdam UMC, University of Amsterdam , 1105 AZ Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6712-9955","authenticated-orcid":false,"given":"Annemieke C","family":"Heijboer","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Endocrine Laboratory, Amsterdam UMC, Vrije Universiteit Amsterdam , 1081 HV Amsterdam ,","place":["The Netherlands"]},{"name":"Amsterdam Gastroenterology, Endocrinology & Metabolism , 1105 AZ Amsterdam ,","place":["The Netherlands"]},{"name":"Department of Laboratory Medicine, Endocrine Laboratory, Amsterdam UMC, University of Amsterdam , 1105 AZ Amsterdam ,","place":["The Netherlands"]},{"name":"Amsterdam Reproduction & Development Research Institute , 1105 AZ Amsterdam ,","place":["The 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