{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:38:45Z","timestamp":1790426325116,"version":"4.1.0"},"reference-count":60,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:p>Activin is a dimeric protein consisting of two similar but distinct \u03b2-subunits, \u03b2A and \u03b2B. In our previous studies, both activin A (\u03b2A\u03b2A) and activin B (\u03b2B\u03b2B) have been demonstrated to stimulate oocyte maturation and promote oocyte maturational competence in the zebrafish. Follistatin, a specific activin-binding protein, can block both activin- and gonadotropin-induced final oocyte maturation in vitro, suggesting that activin is likely a downstream mediator of gonadotropin actions in the zebrafish ovary. In the present study, a full-length cDNA encoding zebrafish ovarian activin \u03b2A was cloned and sequenced. The precursor of zebrafish activin \u03b2A consists of 395 amino acids and its mature region exhibits about 78% homology with that of mammals. Using an in vitro primary culture of the ovarian follicle cells and semiquantitative RT-PCR assays, we examined the regulation of activin \u03b2A and \u03b2B expression by human chorionic gonadotropin (hCG) and its intracellular signal transduction mechanisms. hCG (15 IU\/ml) increased the mRNA level of activin \u03b2A-subunit; however, it significantly down-regulated the steady-state expression level of activin \u03b2B in a time- and dose-dependent manner. The differential regulation of the two \u03b2-subunits by hCG could be mimicked by 3-isobutyl-1-methylxanthine, forskolin, and dibutyryl-cAMP, suggesting involvement of the intracellular cAMP pathway. Interestingly, H89 (a specific inhibitor of protein kinase A, PKA) could effectively block hCG- and forskolin-stimulated activin \u03b2A expression at 10 \u03bcm, but it was unable to reverse the inhibitory effects of hCG and forskolin on \u03b2B expression. This suggests that the hCG-stimulated activin \u03b2A expression is dependent on the activation of the cAMP-PKA pathway, whereas the inhibitory effect of hCG on activin \u03b2B expression is likely mediated by PKA-independent pathway(s).<\/jats:p>","DOI":"10.1210\/en.2002-220734","type":"journal-article","created":{"date-parts":[[2003,1,21]],"date-time":"2003-01-21T21:24:46Z","timestamp":1043184286000},"page":"491-499","source":"Crossref","is-referenced-by-count":57,"title":["Involvement of Cyclic Adenosine 3\u2032,5\u2032-Monophosphate in the Differential Regulation of Activin \u03b2A and \u03b2B Expression by Gonadotropin in the Zebrafish Ovarian Follicle Cells"],"prefix":"10.1210","volume":"144","author":[{"given":"Yajun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071612503877600_R1","first-page":"200","article-title":"Initial and cyclic recruitment of ovarian follicles.","volume":"21","author":"McGee","year":"2000","journal-title":"Endocr Rev"},{"key":"2020071612503877600_R2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0303-7207(99)00219-1","article-title":"Role of LH and FSH in ovarian function.","volume":"161","author":"Howles","year":"2000","journal-title":"Mol Cell Endocrinol"},{"key":"2020071612503877600_R3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1530\/rep.0.1210503","article-title":"Potential local regulatory functions of inhibins, activins and follistatin in the 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