{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:55:11Z","timestamp":1760954111019},"reference-count":35,"publisher":"The Endocrine Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The specific biological function of the cell surface or membrane-bound isoform of colony-stimulating factor-1 (mCSF-1) is not well understood. To help define the role of this isoform in bone, we developed a transgenic mouse in which targeted expression of human mCSF-1 in osteoblasts was achieved under the control of the 2.4-kb rat collagen type I \u03b1 promoter. Bone density, determined by peripheral quantitative computed tomography, was reduced 7% in mCSF-1 transgenic compared with that in wild-type mice. Histomorphometric analyses indicated that the number of osteoclasts in bone (NOc\/BPm, NOc\/TAR, OcS\/BS) was significantly increased in transgenic mice (1.7- to 1.8-fold; P &amp;lt; 0.05 to P &amp;lt; 0.01) compared with that in wild-type animals. Interestingly, the osteoblast-restricted isoform transgene corrected the osteopetrosis seen in CSF-1-deficient op\/op mice. Skeletal growth and bone density in op\/op mice expressing mCSF-1 in osteoblasts were similar to those in wild-type mice and were dramatically different from those in the unmanipulated op\/op animals. The op\/op mice expressing mCSF-1 in bone had normal incisor and molar tooth eruption, whereas the op\/op mice evidenced the expected failure of tooth eruption. These findings directly support the conclusion that mCSF-1 is functionally active in bone in vivo and is probably an important local source of CSF-1.<\/jats:p>","DOI":"10.1210\/en.2002-221071","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T05:35:17Z","timestamp":1052804117000},"page":"3677-3682","source":"Crossref","is-referenced-by-count":24,"title":["The Cell Surface Form of Colony-Stimulating Factor-1 Is Biologically Active in Bone in Vivo"],"prefix":"10.1210","volume":"144","author":[{"given":"Gang-Qing","family":"Yao","sequence":"first","affiliation":[{"name":"Section of Comparative Medicine (G.-Q.Y.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jain-Jun","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (J.-J.W., B.-H.S., M.A.M., K.I.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben-Hua","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (J.-J.W., B.-H.S., M.A.M., K.I.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Troiano","sequence":"first","affiliation":[{"name":"Department of Orthopedics (N.T.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Ann","family":"Mitnick","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (J.-J.W., B.-H.S., M.A.M., K.I.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl","family":"Insogna","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (J.-J.W., B.-H.S., M.A.M., K.I.), Yale University School of Medicine, New Haven, Connecticut 06520-8016"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071613171042200_R1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1146\/annurev.bi.62.070193.002503","article-title":"Membrane-anchored growth factors.","volume":"62","author":"Massague","year":"1993","journal-title":"Annu Rev Biochem"},{"key":"2020071613171042200_R2","doi-asserted-by":"crossref","first-page":"3734","DOI":"10.1182\/blood.V96.12.3734","article-title":"Differential stimulation of c-Kit mutants by membrane-bound and soluble Steel Factor correlates with leukemic potential.","volume":"96","author":"Gommerman","year":"2000","journal-title":"Blood"},{"key":"2020071613171042200_R3","first-page":"761","article-title":"Responses of the murine myeloid cell line FDC-P1 to soluble and membrane-bound forms of steel factor (SLF).","volume":"21","author":"Caruana","year":"1993","journal-title":"Exp Hematol"},{"issue":"Suppl 3","key":"2020071613171042200_R4","first-page":"493","article-title":"Role of the stem cell factor (SCF) receptor and the alternative forms of its ligand (SCF) in the induction of long-term growth by stroma cells","volume":"11","author":"Friel","year":"1997","journal-title":"Leukemia"},{"key":"2020071613171042200_R5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0092-8674(91)90401-J","article-title":"Genetic and structural homology of stem cell factor and macrophage colony-stimulating factor.","volume":"65","author":"Bazan","year":"1991","journal-title":"Cell"},{"key":"2020071613171042200_R6","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1182\/blood.V85.3.641.bloodjournal853641","article-title":"Membrane-bound Steel factor induces more persistent tyrosine kinase activation and longer life span of c-kit gene-encoded protein than its soluble form.","volume":"85","author":"Miyazawa","year":"1995","journal-title":"Blood"},{"key":"2020071613171042200_R7","first-page":"385","author":"Weir","year":"2000"},{"key":"2020071613171042200_R8","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/oxfordjournals.jhered.a108657","article-title":"Osteopetrosis, a new recessive skeletal mutation on chromosome 12 of the mouse.","volume":"67","author":"Marks Jr","year":"1976","journal-title":"J Hered"},{"key":"2020071613171042200_R9","doi-asserted-by":"crossref","first-page":"4828","DOI":"10.1073\/pnas.87.12.4828","article-title":"Total absence of colony-stimulating factor 1 in the macrophage-deficient osteopetrotic (op\/op) mouse.","volume":"87","author":"Wiktor-Jedrzejczak","year":"1990","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071613171042200_R10","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1084\/jem.156.5.1516","article-title":"Hematological characterization of congenital osteopetrosis in op\/op mouse. 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