{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:05:10Z","timestamp":1767855910496,"version":"3.49.0"},"reference-count":22,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,10,1]]},"abstract":"<jats:p>This study used relaxin (RLX) gene knockout mice (Rlx\u2212\/\u2212) to investigate the effects of RLX on myometrial oxytocin receptor (OTR) and estrogen receptor (ER)-\u03b1 gene expression in late gestation. We also characterized the temporal expression of the RLX receptor (LGR7) and demonstrated gene transcripts in the myometrium of Rlx+\/+ and Rlx\u2212\/\u2212 mice. There was a significant (P &amp;lt; 0.05) decrease in myometrial LGR7 gene expression on d 17.5 and 18.5 post coitum (pc) compared with earlier stages of gestation, but no differences between Rlx+\/+ and Rlx\u2212\/\u2212 mice. Myometrial OTR mRNA levels increased at the end of gestation in Rlx+\/+ but not Rlx\u2212\/\u2212 mice. ER\u03b1 gene expression was up-regulated on d 14.5 pc in Rlx+\/+ mice, with mRNA levels remaining high throughout late gestation. In contrast, ER\u03b1 mRNA levels were significantly lower in Rlx\u2212\/\u2212 mice on d 14.5 and 18.5 pc. These data show that the increases in myometrial OTR and ER\u03b1 expression in late pregnant Rlx+\/+ mice were attenuated in Rlx\u2212\/\u2212 mice. The effects of RLX on OTRs are probably mediated via activation of ER\u03b1. Finally, RLX receptor expression in the myometrium of Rlx\u2212\/\u2212 mice did not differ from wild-type mice, implying that RLX does not influence expression of its receptor.<\/jats:p>","DOI":"10.1210\/en.2003-0548","type":"journal-article","created":{"date-parts":[[2003,7,29]],"date-time":"2003-07-29T00:26:38Z","timestamp":1059438398000},"page":"4272-4275","source":"Crossref","is-referenced-by-count":23,"title":["Inhibition of Oxytocin Receptor and Estrogen Receptor-\u03b1 Expression, But Not Relaxin Receptors (LGR7), in the Myometrium of Late Pregnant Relaxin Gene Knockout Mice"],"prefix":"10.1210","volume":"144","author":[{"given":"Andrew L.","family":"Siebel","sequence":"first","affiliation":[{"name":"Department of Zoology and Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen 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