{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:10:02Z","timestamp":1791040202126,"version":"4.1.0"},"reference-count":51,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In dairy goats, treatments associating a progestogen and the equine chorionic gonadotropin (eCG) are the easiest way to induce and synchronize estrus and ovulation and to permit artificial insemination (AI) and\/or out of season breeding. From the first treatment, the injection of eCG induces, in some females, the production of anti-eCG antibodies (Abs) that will interfere with the effectiveness of subsequent treatments. These anti-eCG Abs delay the preovulatory LH surge and the ovulation time, leading to poor fertility of the treated females. In this study, by in vitro bioassays, we show that anti-eCG Abs can positively or negatively modulate the LH and\/or FSH bioactivities of eCG. Moreover, the modulation level of eCG bioactivity does not depend on the anti-eCG Ab affinity for eCG, as shown by surface plasmon resonance technology. The specificity of anti-eCG Abs tested by competitive ELISA highlighted the importance of a glycan environment in the recognition mechanism, especially the sialic acids specific to eCG. The different effects of anti-eCG Abs on eCG bioactivities could be explained by two hypotheses. First, steric hindrance preventing the interaction of eCG with its receptors would explain the inhibitory effect of some anti-eCG Abs; second, a conformational change in eCG by anti-eCG Abs could induce inhibition or potentiation of eCG bioactivities. It is significant that these modulations of eCG bioactivities by anti-eCG Abs impact mainly on the FSH bioactivity of eCG, which is essential for ovarian stimulation and subsequent fertility after treatment and AI, and to a lesser extent on LH bioactivity.<\/jats:p>","DOI":"10.1210\/en.2003-0595","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T00:17:17Z","timestamp":1065485837000},"page":"294-303","source":"Crossref","is-referenced-by-count":53,"title":["Antiequine Chorionic Gonadotropin (eCG) Antibodies Generated in Goats Treated with eCG for the Induction of Ovulation Modulate the Luteinizing Hormone and Follicle-Stimulating Hormone Bioactivities of eCG Differently"],"prefix":"10.1210","volume":"145","author":[{"given":"Virginie","family":"Herve\u0301","sequence":"first","affiliation":[{"name":"Laboratoire Me\u0301canismes d\u2019Action des Gonadotropines, Unite\u0301 Mixte de Recherche 6073, Institut National de la Recherche Agronomique\/Centre National de la Recherche Scientifique\/Universite\u0301 de Tours, Station de Physiologie de la Reproduction des Mammife\u0300res Domestiques, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois","family":"Roy","sequence":"additional","affiliation":[{"name":"Laboratoire Me\u0301canismes d\u2019Action des Gonadotropines, Unite\u0301 Mixte de Recherche 6073, Institut National de la Recherche Agronomique\/Centre National de la Recherche Scientifique\/Universite\u0301 de Tours, Station de Physiologie de la Reproduction des Mammife\u0300res Domestiques, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Bertin","sequence":"additional","affiliation":[{"name":"Laboratoire Me\u0301canismes d\u2019Action des Gonadotropines, Unite\u0301 Mixte de Recherche 6073, Institut National de la Recherche Agronomique\/Centre National de la Recherche Scientifique\/Universite\u0301 de Tours, Station de Physiologie de la Reproduction des Mammife\u0300res Domestiques, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Guillou","sequence":"additional","affiliation":[{"name":"Laboratoire Me\u0301canismes d\u2019Action des Gonadotropines, Unite\u0301 Mixte de Recherche 6073, Institut National de la Recherche Agronomique\/Centre National de la Recherche Scientifique\/Universite\u0301 de Tours, Station de Physiologie de la Reproduction des Mammife\u0300res Domestiques, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Christine","family":"Maurel","sequence":"additional","affiliation":[{"name":"Laboratoire Me\u0301canismes d\u2019Action des Gonadotropines, Unite\u0301 Mixte de Recherche 6073, Institut National de la Recherche Agronomique\/Centre National de la Recherche Scientifique\/Universite\u0301 de Tours, Station de Physiologie de la Reproduction des Mammife\u0300res Domestiques, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071619250817100_R1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1530\/jrf.0.0290313","article-title":"The origin of the equine endometrial cups. 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