{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:38:41Z","timestamp":1791092321735,"version":"4.1.0"},"reference-count":44,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Aldosterone contributes to cardiac failure, which is associated with induction of inflammatory mediators. Moreover, aldosterone was shown to induce a vascular inflammatory phenotype in the rat heart. Using Western blotting and\/or real-time RT-PCR, we examined the effect of aldosterone on the expression of the proinflammatory molecules, cyclooxygenase-2 (COX-2), and IL-6 in neonatal rat ventricular cardiac myocytes and fibroblasts as well as in adult cardiomyocytes after myocardial infarction. In cardiomyocytes, aldosterone induced COX-2 but not IL-6 expression. After 4\u201318 h of stimulation with 1 \u03bcm aldosterone, a significant increase in COX-2 protein expression was observed, preceded by an increase of COX-2 mRNA levels. After 18 h treatment, 100 nm and 1 \u03bcm aldosterone increased COX-2 protein amount by 2- and 4-fold, respectively. Consistently, aldosterone increased by 2.5-fold prostaglandin E2 secretion in cardiomyocytes. In cardiac fibroblasts, aldosterone increased neither COX-2 nor IL-6 mRNA expression. Interestingly, prostaglandin E2 (100 nm) strongly induced both proinflammatory molecules in fibroblasts and cardiomyocytes. Our results indicate that aldosterone directly induces COX-2 expression in cardiomyocytes and suggest that the subsequent increase in prostaglandin secretion may act in an autocrine and\/or paracrine manner inducing in turn COX-2 and IL-6 expression. In vivo, myocardial infarction strongly increased both COX-2 and IL-6 expression in ventricular cardiomyocytes. Administration of the aldosterone antagonist RU28318 completely prevented COX-2 induction by infarction and partially inhibited the increase in IL-6 mRNA. These data suggest that after myocardial infarction, mineralocorticoid receptor activity is responsible for COX-2 induction and indirectly participates in IL-6 expression in cardiomyocytes.<\/jats:p>","DOI":"10.1210\/en.2003-1544","type":"journal-article","created":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T01:24:49Z","timestamp":1080609889000},"page":"3135-3142","source":"Crossref","is-referenced-by-count":24,"title":["Direct and Indirect Effects of Aldosterone on Cyclooxygenase-2 and Interleukin-6 Expression in Rat Cardiac Cells in Culture and after Myocardial Infarction"],"prefix":"10.1210","volume":"145","author":[{"given":"Michela C.","family":"Rebsamen","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Diabetology (M.C.R., C.G.-W., U.L.), University Hospital, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeline","family":"Perrier","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U390-EA 3759 (E.P., J.-P.B.), CHU Arnaud de Villeneuve, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Gerber-Wicht","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Diabetology (M.C.R., C.G.-W., U.L.), University Hospital, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Benitah","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U390-EA 3759 (E.P., J.-P.B.), CHU Arnaud de Villeneuve, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Lang","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Diabetology (M.C.R., C.G.-W., U.L.), University Hospital, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071612390995800_R1","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1210\/jc.2003-030373","article-title":"Aldosterone: direct effects on and production by the heart.","volume":"88","author":"White","year":"2003","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612390995800_R2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1146\/annurev.med.48.1.231","article-title":"Glucocorticoid and mineralocorticoid receptors: biology and clinical relevance.","volume":"48","author":"Funder","year":"1997","journal-title":"Annu Rev Med"},{"key":"2020071612390995800_R3","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1046\/j.1440-1681.2001.03586.x","article-title":"Mineralocorticoids and cardiac fibrosis: the decade in review.","volume":"28","author":"Funder","year":"2001","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"2020071612390995800_R4","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1056\/NEJM199909023411001","article-title":"The effect of spironolactone on morbidity and mortality in patients with severe heart failure. 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