{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:01:52Z","timestamp":1792638112120,"version":"4.5.0"},"reference-count":22,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:p>The development of normal and abnormal glandular structures in the prostate is controlled at the endocrine and paracrine levels by reciprocal interactions between epithelium and stroma. To study these processes, it is useful to have an efficient method of tissue acquisition for reproducible isolation of cells from defined histologies. Here we assessed the utility of a standardized system for acquisition and growth of prostatic cells from different regions of the prostate with different pathologies, and we compared the abilities of stromal cells from normal peripheral zone, benign prostatic hyperplasia (BPH-S), and cancer to induce the growth of a human prostatic epithelial cell line (BPH-1) in vivo. Using the tissue recombination method, we showed that grafting stromal cells (from any histology) alone or BPH-1 epithelial cells alone produced no visible grafts. Recombining stromal cells from normal peripheral zone with BPH-1 cells also produced no visible grafts (n = 15). Recombining BPH-S with BPH-1 cells generated small, well-organized, and sharply demarcated grafts approximately 3\u20134 mm in diameter (n = 9), demonstrating a moderate inductive ability of BPH-S. Recombining stromal cells from cancer with BPH-1 cells generated highly disorganized grafts that completely surrounded the host kidney and invaded into adjacent renal tissue, demonstrating induction of an aggressive phenotype. We conclude that acquisition of tissue from toluidine blue dye-stained specimens is an efficient method to generate high-quality epithelial and\/or stromal cultures. Stromal cells derived by this method from areas of BPH and cancer induce epithelial cell growth in vivo, which mimics the natural history of these diseases.<\/jats:p>","DOI":"10.1210\/en.2004-1123","type":"journal-article","created":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:24:04Z","timestamp":1097195044000},"page":"13-18","source":"Crossref","is-referenced-by-count":105,"title":["A System for Studying Epithelial-Stromal Interactions Reveals Distinct Inductive Abilities of Stromal Cells from Benign Prostatic Hyperplasia and Prostate Cancer"],"prefix":"10.1210","volume":"146","author":[{"given":"Wendy W.","family":"Barclay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph D.","family":"Woodruff","sequence":"first","affiliation":[{"name":"Pathology (R.D.W.), Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Craig","family":"Hall","sequence":"first","affiliation":[{"name":"Urology (M.C.H., S.D.C.), Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Cramer","sequence":"first","affiliation":[{"name":"Urology (M.C.H., S.D.C.), Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157"},{"name":"Departments of Cancer Biology (W.W.B., S.D.C.), Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157"},{"name":"Departments of Cancer Biology (W.W.B., S.D.C.), Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071612555574000_R1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1002\/ar.1091720206","article-title":"Epithelio-mesenchymal interaction in primordial gland structures which become responsive to androgenic stimulation.","volume":"172","author":"Cunha","year":"1972","journal-title":"Anat Rec"},{"key":"2020071612555574000_R2","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/0022-4731(81)90338-1","article-title":"Stromal-epithelial interactions\u2014I. Induction of prostatic phenotype in urothelium of testicular feminized (Tfm\/y) mice.","volume":"14","author":"Cunha","year":"1981","journal-title":"J Steroid Biochem"},{"key":"2020071612555574000_R3","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1083\/jcb.96.6.1662","article-title":"Epithelial-mesenchymal interactions in prostatic development. I. morphological observations of prostatic induction by urogenital sinus mesenchyme in epithelium of the adult rodent urinary bladder.","volume":"96","author":"Cunha","year":"1983","journal-title":"J Cell Biol"},{"key":"2020071612555574000_R4","first-page":"81","article-title":"Androgenic effects upon prostatic epithelium are mediated via trophic influences from stroma.","volume":"145","author":"Cunha","year":"1984","journal-title":"Prog Clin Biol Res"},{"key":"2020071612555574000_R5","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1210\/edrv-8-3-338","article-title":"The endocrinology and developmental biology of the prostate.","volume":"8","author":"Cunha","year":"1987","journal-title":"Endocr Rev"},{"key":"2020071612555574000_R6","doi-asserted-by":"crossref","first-page":"44475","DOI":"10.1074\/jbc.M205361200","article-title":"Rescue of embryonic epithelium reveals that the homozygous deletion of the retinoblastoma gene confers growth factor independence and immortality but does not influence epithelial differentiation of tissue morphogenesis.","volume":"277","author":"Day","year":"2002","journal-title":"J Biol Chem"},{"key":"2020071612555574000_R7","first-page":"5002","article-title":"Carcinma-associated fibroblasts direct tumor progression of initiated human prostate epithelium.","volume":"59","author":"Olumi","year":"1999","journal-title":"Cancer Res"},{"key":"2020071612555574000_R8","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/jcb.10691","article-title":"Are primary cultures realistic models of prostate cancer?","volume":"91","author":"Peehl","year":"2004","journal-title":"J Cell Biochem"},{"key":"2020071612555574000_R9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1002\/(SICI)1097-0045(19980501)35:2<125::AID-PROS6>3.0.CO;2-I","article-title":"Basic FGF, EGF, and PDGF modify TGF\u03b2-induction of smooth muscle cell phenotype in human prostatic stromal cells.","volume":"35","author":"Peehl","year":"1998","journal-title":"Prostate"},{"key":"2020071612555574000_R10","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3109\/08977198908998000","article-title":"In vitro studies of human prostatic epithelial cells: attempts to identify distinguishing features of malignant cells.","volume":"1","author":"Peehl","year":"1989","journal-title":"Growth Factors"},{"key":"2020071612555574000_R11","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1038\/labinvest.3780258","article-title":"Method for procuring specific populations of viable human prostate cells for research.","volume":"81","author":"Fischer","year":"2001","journal-title":"Lab Invest"},{"key":"2020071612555574000_R12","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/BF01797775","article-title":"Serial culture of adult human prostatic epithelial cells.","volume":"9","author":"Peehl","year":"1985","journal-title":"J Tissue Cult Tech"},{"key":"2020071612555574000_R13","first-page":"159","author":"Peehl","year":"1992"},{"key":"2020071612555574000_R14","first-page":"265","article-title":"25-Hydroxyvitamin D3, the prohormone of 1,25-dihydroxyvitamin D3, inhibits the proliferation of primary prostatic epithelial cells.","volume":"9","author":"Barreto","year":"2000","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"2020071612555574000_R15","doi-asserted-by":"crossref","first-page":"3191","DOI":"10.1093\/jn\/131.10.3191","article-title":"Synergistic Inhibition of prostatic epithelial cell growth by genistein and vitamin D.","volume":"132","author":"Rao","year":"2002","journal-title":"J Nutr"},{"key":"2020071612555574000_R16","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1006\/excr.1997.3525","article-title":"Induction of smooth muscle cell phenotype in cultured human prostatic stromal cells.","volume":"232","author":"Peehl","year":"1997","journal-title":"Exp Cell Res"},{"key":"2020071612555574000_R17","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0094-0143(21)00961-7","article-title":"Pathology of benign prostatic hyperplasia. 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