{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T21:04:54Z","timestamp":1776459894513,"version":"3.51.2"},"reference-count":15,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:p>Cushing\u2019s disease is characterized by persistent ACTH secretion under hypercortisolemia. In an attempt to clarify the molecular mechanism, we examined the effect of 11\u03b2-hydroxysteroid dehydrogenase (HSD) inhibition on glucocorticoid suppression of ACTH release using murine corticotroph tumor cells. We found that 11\u03b2-HSD2, as well as -HSD1, was expressed in the cells and that its inhibition by carbenoxolone significantly improved the negative feedback effect of glucocorticoid. Carbenoxolone also enhanced apoptosis induced by cortisol. These effects are most likely attributable to inhibition of 11\u03b2-HSD2 because only cortisol, a substrate of 11\u03b2-HSD2, was present in these experimental conditions. We conclude that ectopic expression of 11\u03b2-HSD2 is, at least in part, responsible for the impaired glucocorticoid suppression in corticotroph adenoma. Inhibition of 11\u03b2-HSD2 may be applicable to the medical therapy for Cushing\u2019s disease.<\/jats:p>","DOI":"10.1210\/en.2005-0544","type":"journal-article","created":{"date-parts":[[2005,10,28]],"date-time":"2005-10-28T00:24:23Z","timestamp":1130459063000},"page":"769-772","source":"Crossref","is-referenced-by-count":19,"title":["Inhibition of 11\u03b2-Hydroxysteroid Dehydrogenase Eliminates Impaired Glucocorticoid Suppression and Induces Apoptosis in Corticotroph Tumor Cells"],"prefix":"10.1210","volume":"147","author":[{"given":"Takeshi","family":"Nigawara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasumasa","family":"Iwasaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Asai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Yoshida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Machiko","family":"Kambayashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sashinami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kozo","family":"Hashimoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Suda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071612510116900_R1","first-page":"45","author":"Majzoub","year":"1995"},{"key":"2020071612510116900_R2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1210\/edrv.20.2.0358","article-title":"The molecular pathogenesis of corticotroph tumors.","volume":"20","author":"Dahia","year":"1999","journal-title":"Endocr Rev"},{"key":"2020071612510116900_R3","first-page":"1088","article-title":"Expression of glucocorticoid receptor gene isoforms in corticotropin-secreting tumors.","volume":"82","author":"Dahia","year":"1997","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612510116900_R4","first-page":"917","article-title":"Human adrenocorticotropin-secreting pituitary adenomas show frequent loss of heterozygosity at the glucocorticoid receptor gene locus.","volume":"83","author":"Huizenga","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612510116900_R5","first-page":"2728","article-title":"Expression of 11\u03b2-hydroxysteroid dehydrogenase isoenzymes in the human pituitary: induction of the type 2 enzyme in corticotropinomas and other pituitary tumors.","volume":"86","author":"Korbonits","year":"2001","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612510116900_R6","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1038\/sj.onc.1206293","article-title":"Abnormal expression of 11\u03b2-hydroxysteroid dehydrogenase type 2 in human pituitary adenomas: a prereceptor determinant of pituitary cell proliferation.","volume":"22","author":"Rabbitt","year":"2003","journal-title":"Oncogene"},{"key":"2020071612510116900_R7","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1210\/endo.138.5.5121","article-title":"Regulation of the rat proopiomelanocortin gene expression in AtT-20 cells. 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