{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T21:47:31Z","timestamp":1774302451172,"version":"3.50.1"},"reference-count":39,"publisher":"The Endocrine Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:p>Chorion is the most abundant site of 11\u03b2-hydroxysteroid dehydrogenase type 1 (11\u03b2-HSD1) expression within intrauterine tissues. It is important to study the regulation of 11\u03b2-HSD1 expression in the chorion in terms of local cortisol production during pregnancy. Using real-time PCR and enzyme activity assay, we found that cortisol (1 \u03bcm) and IL-1\u03b2 (10 ng\/ml) for 24 h significantly increased 11\u03b2-HSD1 mRNA expression and reductase activity in cultured human chorionic trophoblasts. A further significant increase of 11\u03b2-HSD1 mRNA expression and reductase activity was observed with cotreatment of cortisol and IL-1\u03b2. To explore the mechanism of induction, 11\u03b2-HSD1 promoter was cloned into pGL3 plasmid expressing a luciferase reporter gene. By transfecting the constructed vector into WISH cells, an amnion-derived cell line, we found that cortisol (1 \u03bcm) or IL-1\u03b2 (10 ng\/ml) significantly increased reporter gene expression. Likewise, an additional increase in reporter gene expression was observed with cotreatment of cortisol and IL-\u03b2. To explore the physiological significance of 11\u03b2-HSD1 induction in the chorion, we studied the effect of cortisol on cytosolic phospholipase A2 and cyclooxygenase 2 expression. We found that treatment of chorionic trophoblast cells with cortisol (1 \u03bcm) induced both cytosolic phospholipase A2 and cyclooxygenase 2 mRNA expression. We conclude that cortisol up-regulates 11\u03b2-HSD1 expression through induction of promoter activity, and the effect was enhanced by IL-1\u03b2, suggesting that more biologically active glucocorticoids could be generated in the fetal membranes in the presence of infection, which may consequently feed forward in up-regulation of prostaglandin synthesis.<\/jats:p>","DOI":"10.1210\/en.2005-1626","type":"journal-article","created":{"date-parts":[[2006,2,10]],"date-time":"2006-02-10T01:33:25Z","timestamp":1139535205000},"page":"2490-2495","source":"Crossref","is-referenced-by-count":46,"title":["Enhancement of Cortisol-Induced 11\u03b2-Hydroxysteroid dehydrogenase Type 1 Expression by Interleukin 1\u03b2 in Cultured Human Chorionic Trophoblast 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