{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T02:47:46Z","timestamp":1771469266985,"version":"3.50.1"},"reference-count":31,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,7,1]]},"abstract":"<jats:p>CRH targets the human myometrium during pregnancy. The efficiency of CRH actions is determined by expression of functional receptors (CRH-R), which are dynamically regulated. Studies in myometrial tissue biopsies using quantitative RT-PCR demonstrated that the onset of labor, term or preterm, is associated with a significant 2- to 3-fold increase in CRH-R1 mRNA levels. Detailed analysis of myometrial CRH-R1 mRNA variants showed a decline of the pro-CRH-R1 mRNA encoding the CRH-R1\u03b2 variant during labor and increased mRNA levels of CRH-R1d mRNA. Studies in myometrial cells identified IL-1\u03b2 as an important regulator of myometrial CRH-R1 gene expression because prolonged treatment of myometrial cells with IL-1\u03b2 (1 ng\/ml) for 18 h induced expression of CRH-R1 mRNA levels by 1.5- to 2-fold but significantly attenuated CRH-R1\u03b2 mRNA expression by 70%. In contrast, IL-1\u03b2 had no effect on CRH-R1d mRNA expression. Studies using specific inhibitors suggest that ERK1\/2, p38 MAPK, and downstream nuclear translocation of nuclear factor-\u03baB mediate IL-1\u03b2 effects on myometrial CRH-R1 gene. However, the increased CRH-R1 mRNA expression was associated with a dampening of the receptor efficacy to activate the adenylyl cyclase\/cAMP signaling cascade. Thus, our findings suggest that IL-1\u03b2 is an important regulator of CRH-R1 expression and functional activity, and this interaction might play a role in the transition of the uterus from quiescence to active contractions necessary for the onset of parturition.<\/jats:p>","DOI":"10.1210\/en.2007-0095","type":"journal-article","created":{"date-parts":[[2007,4,13]],"date-time":"2007-04-13T00:24:55Z","timestamp":1176423895000},"page":"3205-3213","source":"Crossref","is-referenced-by-count":37,"title":["The Onset of Labor Alters Corticotropin-Releasing Hormone Type 1 Receptor Variant Expression in Human Myometrium: Putative Role of Interleukin-1\u03b2"],"prefix":"10.1210","volume":"148","author":[{"given":"Danijela","family":"Markovic","sequence":"first","affiliation":[{"name":"Endocrinology and Metabolism (D.M., M.V., M.G., D.S., H.L., D.K.G.), Warwick Medical School, University of Warwick, Coventry CV4 7AL, United 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