{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:22:24Z","timestamp":1773418944476,"version":"3.50.1"},"reference-count":39,"publisher":"The Endocrine Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,11,1]]},"abstract":"<jats:p>Angiotensin II (AngII) stimulates aldosterone biosynthesis in the zona glomerulosa of the adrenal cortex. AngII also triggers the MAPK pathways (ERK1\/2 and p38). Because ERK1\/2 phosphorylation is a transient process, phosphatases could play a crucial role in the acute steroidogenic response. Here we show that the dual specificity (threonine\/tyrosine) MAPK phosphatase-1 (MKP-1) is present in bovine adrenal glomerulosa cells in primary culture and that AngII markedly increases its expression in a time- and concentration-dependent manner (IC50 = 1 nm), a maximum of 548 \u00b1 10% of controls being reached with 10 nm AngII after 3 h (n = 3, P &amp;lt; 0.01). This effect is completely abolished by losartan, a blocker of the AT1 receptor subtype. Moreover, this AngII-induced MKP-1 expression is reduced to 250 \u00b1 35% of controls (n = 3, P &amp;lt; 0.01) in the presence of U0126, an inhibitor of ERK1\/2 phosphorylation, suggesting an involvement of the ERK1\/2 MAPK pathway in MKP-1 induction. Indeed, shortly after AngII-induced phosphorylation of ERK1\/2 (220% of controls at 30 min), MKP-1 protein expression starts to increase. This increase is associated with a reduction in ERK1\/2 phosphorylation, which returns to control values after 3 h of AngII challenge. Enhanced MKP-1 expression is essentially due to a stabilization of MKP-1 mRNA. AngII treatment leads to a 53-fold increase in phosphorylated MKP-1 levels and a doubling of MKP-1 phosphatase activity. Overexpression of MKP-1 results in decreased phosphorylation of ERK1\/2 and aldosterone production in response to AngII stimulation. These results strongly suggest that MKP-1 is the specific phosphatase induced by AngII and involved in the negative feedback mechanism ensuring adequate ERK1\/2-mediated aldosterone production in response to the hormone.<\/jats:p>","DOI":"10.1210\/en.2007-0241","type":"journal-article","created":{"date-parts":[[2007,8,10]],"date-time":"2007-08-10T00:35:28Z","timestamp":1186706128000},"page":"5573-5581","source":"Crossref","is-referenced-by-count":13,"title":["Angiotensin II-Induced Mitogen-Activated Protein Kinase Phosphatase-1 Expression in Bovine Adrenal Glomerulosa Cells: Implications in Mineralocorticoid Biosynthesis"],"prefix":"10.1210","volume":"148","author":[{"given":"Andre\u0301s J.","family":"Casal","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Diabetology and Nutrition (A.J.C., A.M.C., C.F.W.-B.), University Hospital, CH-1211 Geneva 14, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ste\u0301phane","family":"Ryser","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics (S.R.), University Hospital, CH-1211 Geneva 14, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro M.","family":"Capponi","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Diabetology and Nutrition (A.J.C., A.M.C., C.F.W.-B.), University Hospital, CH-1211 Geneva 14, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carine F.","family":"Wang-Buholzer","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Diabetology and Nutrition (A.J.C., A.M.C., C.F.W.-B.), University Hospital, CH-1211 Geneva 14, 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