{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:55:30Z","timestamp":1792468530412,"version":"4.5.0"},"reference-count":59,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,4,1]]},"abstract":"<jats:p>The Ca2+-dependent precursor convertase furin is abundantly expressed in epidermal keratinocytes and melanocytes. In this context, it is noteworthy that proopiomelanocortin (POMC) cleavage is also processed by furin, leading to ACTH, \u03b2-lipotropin, and \u03b2-endorphin. All prohormone convertases including furin are regulated by Ca2+. Because numerous epidermal peptides and enzymes are affected by H2O2-mediated oxidation, including the POMC-derived peptides \u03b1-MSH and \u03b2-endorphin as shown in the epidermis of patients with vitiligo, we here asked the question of whether furin could also be a possible target for this oxidation mechanism by using immunofluorescence, RT-PCR, Western blotting, Ca2+-binding studies, and computer modeling. Our results demonstrate significantly decreased in situ immunoreactivity of furin in the epidermis of patients with progressive vitiligo (n = 10), suggesting H2O2-mediated oxidation. This was confirmed by 45Ca2+-binding studies with human recombinant furin identifying the loss of one Ca2+-binding site from the enzyme after oxidation with H2O2. Computer simulation supported alteration of one of the two Ca2+-binding sites on furin. Taken together, our results implicate that the Ca2+-dependent proteolytic activity of this convertase is targeted by H2O2, which in turn could contribute to the reduced epidermal expression of the POMC-derived peptides \u03b1-MSH and \u03b2-endorphin as documented earlier in patients with vitiligo.<\/jats:p>","DOI":"10.1210\/en.2007-1317","type":"journal-article","created":{"date-parts":[[2008,1,3]],"date-time":"2008-01-03T20:34:09Z","timestamp":1199392449000},"page":"1638-1645","source":"Crossref","is-referenced-by-count":27,"title":["The Ca2+-Binding Capacity of Epidermal Furin Is Disrupted by H2O2-Mediated Oxidation in Vitiligo"],"prefix":"10.1210","volume":"149","author":[{"given":"J. D.","family":"Spencer","sequence":"first","affiliation":[{"name":"Clinical and Experimental Dermatology\/Department of Biomedical Sciences (J.D.S., N.C.J.G., K.U.S.), University of Bradford, and Institute for Pigmentary Disorders in Association with the EM Arndt University Greifswald, Germany and University of Bradford, Bradford, BD7 1DP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. C. J.","family":"Gibbons","sequence":"first","affiliation":[{"name":"Clinical and Experimental Dermatology\/Department of Biomedical Sciences (J.D.S., N.C.J.G., K.U.S.), University of Bradford, and Institute for Pigmentary Disorders in Association with the EM Arndt University Greifswald, Germany and University of Bradford, Bradford, BD7 1DP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bo\u0308hm","sequence":"first","affiliation":[{"name":"Department of Dermatology (M.B.), University of Mu\u0308nster, D-41849 Mu\u0308nster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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