{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:22:00Z","timestamp":1773271320498,"version":"3.50.1"},"reference-count":37,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:p>The antiapoptotic molecule Fas-associated death domain-like IL-1\u03b2-converting enzyme inhibitory protein (FLIP) inhibits Fas-mediated apoptosis by blocking activation of caspase-8. We previously showed that expression of transgenic FLIP on thyroid epithelial cells (TECs) of DBA\/1 and CBA\/J mice promoted earlier resolution of granulomatous experimental autoimmune thyroiditis in vivo. This study was undertaken to directly determine whether transgenic FLIP expressed on cultured TECs can protect TECs from Fas-mediated apoptosis in vitro. The results indicate that cultured TECs from DBA\/1 and CBA\/J mice can be sensitized in vitro by interferon-\u03b3 and TNF-\u03b1 to undergo Fas-mediated apoptosis. Transgenic overexpression of FLIP protected cultured TECs of FLIP transgene (Tg)+ DBA\/1 and CBA\/J mice from Fas-mediated apoptosis, and FLIP small interfering RNA transfection of cultured TECs of FLIP Tg+ DBA\/1 and CBA\/J mice abolished the protective effect. These in vitro results are consistent with our previous in vivo studies using DBA\/1 and CBA\/J FLIP Tg+ mice and provide direct support for the hypothesis that transgenic expression of FLIP promotes resolution of granulomatous experimental autoimmune thyroiditis by protecting TECs from apoptosis.<\/jats:p>","DOI":"10.1210\/en.2008-0080","type":"journal-article","created":{"date-parts":[[2008,3,21]],"date-time":"2008-03-21T01:05:58Z","timestamp":1206061558000},"page":"3321-3329","source":"Crossref","is-referenced-by-count":20,"title":["Cultured Murine Thyroid Epithelial Cells Expressing Transgenic Fas-Associated Death Domain-Like Interleukin-1\u03b2 Converting Enzyme Inhibitory Protein Are Protected from Fas-Mediated Apoptosis"],"prefix":"10.1210","volume":"149","author":[{"given":"Yujiang","family":"Fang","sequence":"first","affiliation":[{"name":"From Research Service (Y.F., H.B.-M.), Harry S. 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