{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:38:46Z","timestamp":1791092326815,"version":"4.1.0"},"reference-count":49,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,1]]},"abstract":"<jats:p>Aldosterone (ALDO) may induce cardiac hypertrophy by nonhemodynamic mechanisms that are not completely defined. Cardiotrophin-1 (CT-1) is a cytokine that exerts hypertrophic actions on isolated cardiomyocytes and promotes cardiac hypertrophy in vivo. We investigated whether ALDO induces CT-1 expression in HL-1 cardiomyocytes aiming at the possibility that the cytokine is involved in ALDO-induced cardiomyocyte hypertrophy. mRNA and protein expression were quantified by RT-PCR and Western blot. Cardiomyocyte area, as an index of hypertrophy, was assayed by image analysis in phalloidin-stained HL-1 cells. ALDO addition to adult HL-1 cardiomyocytes increased (P &amp;lt; 0.01) CT-1 mRNA and protein expression in a concentration-dependent manner. This effect was abrogated by actinomycin D, the mineralocorticoid and glucocorticoid receptor antagonists spironolactone and RU486, respectively, and the p38 MAPK blocker SB203580. CT-1 signaling pathway blockade with specific antibodies against the cytokine and its two receptor subunits avoided (P &amp;lt; 0.01) \u03b1-sarcomeric actin and c-fos protein overexpression as well as cell size increase induced by ALDO in HL-1 cells. In vivo, a single ALDO injection acutely increased (P &amp;lt; 0.01) the myocardial expression of CT-1 in C57BJ6 wild-type mice but not CT-1-null mice. The bolus of the mineralocorticoid increased (P &amp;lt; 0.01) ANP and c-fos mRNA expression in the myocardium of wild-type mice, whereas no changes were observed in CT-1-null mice. In summary, ALDO induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms. CT-1 up-regulation could have relevance in the direct hypertrophic effects of ALDO in cardiomyocytes.<\/jats:p>","DOI":"10.1210\/en.2008-0120","type":"journal-article","created":{"date-parts":[[2008,6,20]],"date-time":"2008-06-20T00:55:20Z","timestamp":1213923320000},"page":"4970-4978","source":"Crossref","is-referenced-by-count":42,"title":["Aldosterone Induces Cardiotrophin-1 Expression in HL-1 Adult Cardiomyocytes"],"prefix":"10.1210","volume":"149","author":[{"given":"Natalia","family":"Lo\u0301pez-Andre\u0301s","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Sciences (N.L.-A., C.I., I.G., J.D., M.A.F.), University Clinic, University of Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"In\u0303igo","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Sciences (N.L.-A., C.I., I.G., J.D., M.A.F.), University Clinic, University of Navarra, 31008 Pamplona, 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