{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:50:48Z","timestamp":1787784648970,"version":"build-2784847793"},"reference-count":44,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>An increase in intracellular Ca2+ ([Ca2+]i) as a result of release of Ca2+ from intracellular stores or influx of extracellular Ca2+ contributes to the regulation of smooth muscle contractile activity. Human uterine smooth muscle cells exhibit receptor-, store-, and diacylglycerol (OAG)-mediated extracellular Ca2+-dependent increases in [Ca2+]i (SRCE) and express canonical transient receptor potential-like channels (TRPC) mRNAs (predominantly TRPC1, -4, and -6) that have been implicated in SRCE. To determine the role of TRPC6 in human myometrial SRCE, short hairpin RNA constructs were designed that effectively targeted a TRPC6 mRNA reporter for degradation. One sequence was used to produce an adenovirus construct (TC6sh1). TC6sh1 reduced TRPC6 mRNA but not TRPC1, -3, -4, -5, or -7 mRNAs in PHM1-41 myometrial cells. Compared with uninfected cells or cells infected with empty vector, the increase in [Ca2+]i in response to OAG was specifically inhibited by TC6sh1, whereas SRCE responses elicited by either oxytocin or thapsigargin were not changed. Similar findings were observed in primary pregnant human myometrial cells. When PHM1-41 cells were activated by OAG in the absence of extracellular Na+, the increase in [Ca2+]i was partially reduced. Furthermore, pretreatment with nifedipine, an L-type calcium channel blocker, also partially reduced the OAG-induced [Ca2+]i increase. Similar effects were observed in primary human myometrial cells. These findings suggest that OAG activates channels containing TRPC6 in myometrial cells and that these channels act via both enhanced Na+ entry coupled to activation of voltage-dependent Ca2+ entry channels and a nifedipine-independent Ca2+ entry mechanism to promote elevation of intracellular Ca2+.<\/jats:p>","DOI":"10.1210\/en.2009-0085","type":"journal-article","created":{"date-parts":[[2009,11,26]],"date-time":"2009-11-26T05:41:47Z","timestamp":1259214107000},"page":"406-416","source":"Crossref","is-referenced-by-count":14,"title":["Attenuation of Canonical Transient Receptor Potential-Like Channel 6 Expression Specifically Reduces the Diacylglycerol-Mediated Increase in Intracellular Calcium in Human Myometrial Cells"],"prefix":"10.1210","volume":"151","author":[{"given":"Daesuk","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences (D.C., Y.-S.K., J.N.P., A.U., C.-Y.K., B.M.S.), Colorado State University, Fort Collins, Colorado 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