{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:07:29Z","timestamp":1780344449721,"version":"3.54.1"},"reference-count":44,"publisher":"The Endocrine Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>Without the vitamin D receptor (VDR), adult mammals develop reduced intestinal calcium absorption, rickets, and osteomalacia. Intestinal calcium absorption normally increases during pregnancy so that the mother can supply sufficient calcium to her fetuses. The maternal skeleton is rapidly resorbed during lactation to provide calcium needed for milk; that lost bone mineral content (BMC) is completely restored after weaning. We studied Vdr null mice to determine whether these adaptations during pregnancy and lactation require the VDR. Vdr nulls were severely rachitic at 10 wk of age on a normal diet. Pregnancy induced a 158% increase in Vdr null BMC to equal the pregnant wild-type (WT) value. Lactation caused BMC losses that were equal in Vdr nulls and WT. Vdr nulls recovered after weaning to a BMC 50% higher than before pregnancy and equal to WT. Additional analyses showed that during pregnancy, duodenal 45Ca absorption increased in Vdr nulls, secondary hyperparathyroidism lessened, bone turnover markers decreased, and osteoid became fully mineralized. A genome-wide microarray analysis of duodenal RNA found marked reduction of Trpv6 in Vdr nulls at baseline but a 13.5-fold increase during pregnancy. Calbindin D-9K (S100g) and Ca2+-ATPase (Pmca1) were not altered by pregnancy. Several other solute transporters increased during pregnancy in Vdr nulls. In summary, Vdr nulls adapt to pregnancy by up-regulating duodenal Trpv6 and intestinal 45Ca absorption, thereby enabling rapid normalization of BMC during pregnancy. These mice lactate normally and fully restore BMC after weaning. Therefore, VDR is not required for the skeletal adaptations during pregnancy, lactation, and after weaning.<\/jats:p>","DOI":"10.1210\/en.2009-1010","type":"journal-article","created":{"date-parts":[[2010,1,6]],"date-time":"2010-01-06T01:45:00Z","timestamp":1262742300000},"page":"886-895","source":"Crossref","is-referenced-by-count":118,"title":["Pregnancy Up-Regulates Intestinal Calcium Absorption and Skeletal Mineralization Independently of the Vitamin D Receptor"],"prefix":"10.1210","volume":"151","author":[{"given":"Neva J.","family":"Fudge","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Memorial University of Newfoundland, St. John\u2019s, Newfoundland, Canada A1B 3V6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"Kovacs","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Memorial University of Newfoundland, St. John\u2019s, Newfoundland, Canada A1B 3V6"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2010,1,5]]},"reference":[{"key":"2020071612262016400_R1","first-page":"141","author":"Bikle","year":"2008"},{"key":"2020071612262016400_R2","first-page":"329","author":"Lips","year":"2008"},{"key":"2020071612262016400_R3","first-page":"832","article-title":"Maternal-fetal calcium and bone metabolism during pregnancy, puerperium and lactation.","volume":"18","author":"Kovacs","year":"1997","journal-title":"Endocr Rev"},{"key":"2020071612262016400_R4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ecl.2005.09.004","article-title":"Calcium and bone disorders during pregnancy and lactation.","volume":"35","author":"Kovacs","year":"2006","journal-title":"Endocrinol Metab Clin North Am"},{"key":"2020071612262016400_R5","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1093\/ajcn\/88.2.520S","article-title":"Vitamin D in pregnancy and lactation: maternal, fetal, and neonatal outcomes from human and animal studies.","volume":"88","author":"Kovacs","year":"2008","journal-title":"Am J Clin Nutr"},{"key":"2020071612262016400_R6","first-page":"2344","article-title":"Calcium and bone metabolism in pregnancy and lactation.","volume":"86","author":"Kovacs","year":"2001","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612262016400_R7","first-page":"E64","article-title":"Calcium transport in small intestine during pregnancy and lactation","volume":"239","author":"Halloran","year":"1980","journal-title":"Am J Physiol"},{"key":"2020071612262016400_R8","first-page":"G631","article-title":"Vitamin D-independent intestinal calcium and phosphorus absorption during reproduction","volume":"259","author":"Brommage","year":"1990","journal-title":"Am J Physiol"},{"key":"2020071612262016400_R9","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1210\/endo-107-6-1923","article-title":"Skeletal changes during pregnancy and lactation: the role of vitamin D.","volume":"107","author":"Halloran","year":"1980","journal-title":"Endocrinology"},{"key":"2020071612262016400_R10","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/BF02411245","article-title":"Role of vitamin D in maternal skeletal changes during pregnancy and lactation: a histomorphometric study.","volume":"34","author":"Miller","year":"1982","journal-title":"Calcif Tissue Int"},{"key":"2020071612262016400_R11","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s10911-005-5394-0","article-title":"Calcium and bone metabolism during pregnancy and lactation.","volume":"10","author":"Kovacs","year":"2005","journal-title":"J Mammary Gland Biol Neoplasia"},{"key":"2020071612262016400_R12","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1023\/A:1022800716196","article-title":"The evolutionary origins of maternal calcium and bone metabolism during lactation.","volume":"7","author":"Wysolmerski","year":"2002","journal-title":"J Mammary Gland Biol Neoplasia"},{"key":"2020071612262016400_R13","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1138\/20070268","article-title":"Conversations between breast and bone: physiological bone loss during lactation as evolutionary template for osteolysis in breast cancer and pathological bone loss after menopause.","volume":"4","author":"Wysolmerski","year":"2007","journal-title":"BoneKEy"},{"key":"2020071612262016400_R14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.bone.2008.09.017","article-title":"Do osteocytes contribute to bone mineral homeostasis? 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