{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:04:20Z","timestamp":1790759060050,"version":"4.1.0"},"reference-count":51,"publisher":"The Endocrine Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:p>Vitamin D insufficiency is a global health issue. Although classically associated with rickets, low vitamin D levels have also been linked to aberrant immune function and associated health problems such as inflammatory bowel disease (IBD). To test the hypothesis that impaired vitamin D status predisposes to IBD, 8-wk-old C57BL\/6 mice were raised from weaning on vitamin D-deficient or vitamin D-sufficient diets and then treated with dextran sodium sulphate (DSS) to induce colitis. Vitamin D-deficient mice showed decreased serum levels of precursor 25-hydroxyvitamin D3 (2.5 \u00b1 0.1 vs. 24.4 \u00b1 1.8 ng\/ml) and active 1,25-dihydroxyvitamin D3 (28.8 \u00b1 3.1 vs. 45.6 \u00b1 4.2 pg\/ml), greater DSS-induced weight loss (9 vs. 5%), increased colitis (4.71 \u00b1 0.85 vs. 1.57 \u00b1 0.18), and splenomegaly relative to mice on vitamin D-sufficient chow. DNA array analysis of colon tissue (n = 4 mice) identified 27 genes consistently (P &amp;lt; 0.05) up-regulated or down-regulated more than 2-fold in vitamin D-deficient vs. vitamin D-sufficient mice, in the absence of DSS-induced colitis. This included angiogenin-4, an antimicrobial protein involved in host containment of enteric bacteria. Immunohistochemistry confirmed that colonic angiogenin-4 protein was significantly decreased in vitamin D-deficient mice even in the absence of colitis. Moreover, the same animals showed elevated levels (50-fold) of bacteria in colonic tissue. These data show for the first time that simple vitamin D deficiency predisposes mice to colitis via dysregulated colonic antimicrobial activity and impaired homeostasis of enteric bacteria. This may be a pivotal mechanism linking vitamin D status with IBD in humans.<\/jats:p>","DOI":"10.1210\/en.2010-0089","type":"journal-article","created":{"date-parts":[[2010,4,15]],"date-time":"2010-04-15T01:34:10Z","timestamp":1271295250000},"page":"2423-2432","source":"Crossref","is-referenced-by-count":231,"title":["Vitamin D Deficiency in Mice Impairs Colonic Antibacterial Activity and Predisposes to Colitis"],"prefix":"10.1210","volume":"151","author":[{"given":"Venu","family":"Lagishetty","sequence":"first","affiliation":[{"name":"Orthopaedic Hospital Research Center (V.L., N.Q.L., T.S.L., R.F.C., J.S.A., M.H.) 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